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Staminodes influence pollen removal and deposition rates in nectar-rewarding self-incompatible Phanera yunnanensis (Caesalpinioideae)

  • Menglin Wang (a1) (a2) (a3), Shuyin Huang (a1), Manru Li (a1) (a2), Doyle McKey (a4) and Ling Zhang (a1)...


Staminodes are sterile stamens that produce no pollen, exhibit diverse structures and perform various functions. Flowers of Phanera yunnanensis possess three fertile stamens with large anthers and long filaments, and seven staminodes with tiny anthers and short filaments. To investigate the adaptive significance of staminodes in this species, we studied effects of staminode removal on pollen removal and deposition, flower visitation rate and fruit set in Xishuangbanna, south-western China. Four species of nectar-foraging pollinators visited flowers, mostly Amegilla zonata and Apis cerana (2.80 ± 0.15 and 1.76 ± 0.41 visits h−1 per flower, respectively). Staminode removal did not affect fruit set, but increased visitation by A. cerana by 2.6-fold, reduced visitation by A. zonata by 68% and increased the pollen removal rate for both pollinators (all effects were significant). Staminode removal significantly reduced pollen deposition rate for A. zonata, but not for A. cerana. These results suggest that the staminodes of P. yunnanensis filter which insects act as pollinators and affect pollen removal and deposition rates. By reducing pollen removal rates, staminodes may implement a pollen-dispensing schedule that spreads pollen dispersal from individual flowers over multiple pollinators. By altering pollen deposition rates, staminodes may influence reproductive fitness in other ways.


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Barrett, SCH (2002) Sexual interference of the floral kind. Heredity 88, 154159.
Cane, JH (1993) Reproductive role of sterile pollen in cryptically dioecious species of flowering plants. Current Science 65, 223225.
Chen, DCH, Zhang, DX, Larsen, K and Larsen, SS (2010) Bauhinia. Flora of China 10, 621.
Cronquist, A (1981) An Integrated System of Classification of Flowering Plants. New York: Columbia University Press, 1262 pp.
Cruden, RWR (1977) Pollen-ovule ratios: a conservative indicator of breeding systems in flowering plants. Evolution 31, 3246.
Dafni, A and Maués, MM (1998) A rapid and simple procedure to determine stigma receptivity. Sexual Plant Reproduction 11, 177180.
Dieringer, G and Cabrera, LR (2002) The interaction between pollinator size and the bristle staminode of Penstemon digitalis (Scrophulariaceae). American Journal of Botany 89, 991997.
Duffy, KJ and Johnson, SD (2015) Staminal hairs enhance fecundity in the pollen-rewarding self-incompatible lily Bulbine abyssinica. Botanical Journal of the Linnean Society 177, 481490.
Endress, PK (1984) The role of inner staminodes in the floral display of some relic Magnoliales. Plant Systematics and Evolution 146, 269282.
Gleiser, G, Internicol, AAI, Austerlitz, F and Bernasconi, G (2014) Stabilizing selection on nectar concentration in wild Petunia axillaris, as revealed by genetic analysis of pollen dispersal. Evolutionary Ecology 28, 869884.
Guimarães, E, Di Stasi, LC and Mainoni-Rodella, RDCS (2008) Pollination biology of Jacaranda oxyphylla with an emphasis on staminode function. Annals of Botany 102, 699711.
Hao, G, Zhang, DX, Zhang, MY, Guo, LX and Li, SJ (2003) Phylogenetics of Bauhinia subgenus Phanera (Leguminosae: Caesalpinioideae) based on ITS sequences of nuclear ribosomal DNA. Botanical Bulletin of Academia Sinica 44, 223228.
Harder, LD and Thomson, JD (1989) Evolutionary options for maximizing pollen dispersal of animal-pollinated plants. American Naturalist 133, 323344.
Hawk, JL and Tepedino, VJ (2007) The effect of staminode removal on female reproductive success in a Wyoming population of the endangered Blowout Penstemon, Penstemon haydenii (Scrophulariaceae). Madroño 54, 2226.
Hokche, O and Ramirez, N (1990) Pollination ecology of seven species of Bauhinia L. (Leguminosae: Caesalpinioideae). Annals of the Missouri Botanical Garden 77, 559572.
Hu, SY (1993) Experimental methods in plant embryology (i) determination of pollen viability. Chinese Bulletin of Botany 10, 6062.
Jin, H, Jiao, GJ and Chen, G (2015) Observation on flowering process, floral morphology and flower visiting insects of Melastoma candidum f. albiflorum. Journal of Plant Resources and Environment 24, 7379.
Kaul, V and Koul, AK (2012) Staminal variation and its possible significance in Commelina benghalensis L. and Commelina caroliniana Walter. Current Science 103, 419426.
Lau, CPY, Saunders, RMK and Ramsden, L (2009) Floral biology, breeding systems and population genetic structure of three climbing Bauhinia species (Leguminosae: Caesalpinioideae) in Hong Kong, China. Journal of Tropical Ecology 25, 147159.
Ley, AC and Claßen-Bockhoff, R (2012) Floral synorganization and its influence on mechanical isolation and autogamy in Marantaceae. Botanical Journal of the Linnean Society 168, 300322.
Lieftinck, MA (1968) A review of Old World species of Thyreus Panzer (= Crocisa Jurine) (Hym., Apoidea, Anthophoridae) Part 4. Palearctic species. Zoologische Verhandelingen 98, 1139.
Lunau, K (2000) The ecology and evolution of visual pollen signals. Plant Systematics and Evolution 222, 89111.
Luo, C, Li, K, Chen, Y, Sun, Y and Yang, W (2007) Pollen viability, stigma receptivity and reproductive features of Jatropha curcas L. (Euphorbiaceae). Acta Botanica Boreali-Occidentalia Sinica 27, 19942001. (In Chinese with English abstract.)
Neto, HFP (2013) Floral biology and breeding system of Bauhinia forficata (Leguminosae: Caesalpinioideae), a moth-pollinated tree in southeastern Brazil. Brazilian Journal of Botany 36, 5564.
Ramirez, N, Sobrevil, AC, Enbech, NXD and Ruiz-Zapata, T (1984) Floral biology and breeding system of Bauhinia benthamiana Taub.(Leguminosae), a bat-pollinated tree in Venezuelan “llanos”. American Journal of Botany 71, 273280.
Ronse Decraene, LP and Smets, EF (2001) Staminodes: their morphological and evolutionary significance. Botanical Review 67, 351402.
Sandvik, SM and Totland, Ø (2003) Quantitative importance of staminodes for female reproductive success in Parnassia palustris under contrasting environmental conditions. Canadian Journal of Botany 81, 4956.
Schlessman, MA (1986) Floral protogyny, self-compatibility and the pollination of Ourisia macrocarpa (Scrophulariaceae). New Zealand Journal of Botany 24, 651656.
Sinou, C, Forest, F, Lewis, GP and Bruneau, A (2009) The genus Bauhinia s.l. (Leguminosae): a phylogeny based on the plastid Trn L–Trn F region. Botany 87, 947960.
Verma, S, Magotra, R and Koul, AK (2004) Stylar movement avoids self pollination and promotes cross pollination in Eremurus himalaicus. Current Science 87, 872873.
Vogel, S (1954) Blütenbiologische Typen als Elemente der Sippengliederung. Botanical Studies 1, 1338.
Walker-Larsen, J and Harder, LD (2000) The evolution of staminodes in angiosperms: patterns of stamen reduction, loss, and functional re-invention. American Journal of Botany 87, 13671384.
Walker-Larsen, J and Harder, LD (2001) Vestigial organs as opportunities for functional innovation: the example of the Penstemon staminode. Evolution 55, 477487.
Wunderlin, RP (2010a) Reorganization of the Cercideae (Fabaceae: Caesalpinioideae). Phytoneuron 48, 15.
Wunderlin, RP (2010b) New combinations in Schnella (Fabaceae: Caesalpiniodeae: Cercideae). Phytoneuron 49, 15.
Wunderlin, RP (2011) New combination in Phanera (Fabaceae). Phytoneuron 19, 12.
Zhang, Z (2013) Reproductive Ecology of Phalaenopsis pulcherrima (Orchidaceae), a Species Endemic to East Asia. PhD dissertation. Hai Nan University, Haikou, China. (In Chinese with English abstract.)



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