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A study of glycogen and lactate in the myotomal muscles and liver of the Coalfish (Gadus virens L.) during sustained swimming

Published online by Cambridge University Press:  11 May 2009

I. A. Johnston  and
G. Goldspink
I. A. Johnston
Affiliation:
Muscle Research Laboratory, Department of Zoology, University of Hull, Hull, Yorkshire
G. Goldspink
Affiliation:
Muscle Research Laboratory, Department of Zoology, University of Hull, Hull, Yorkshire
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Extract

The locomotor roles of the myotomal muscles of fish are dependent on swimming speed. The mean maximum sustained swimming speed for coalfish (Gadus virens L.) during a 6-h period in an experimental exercise chamber was determined using a fixedvelocity technique and found to be 4 bodylengths/s. Biochemical measurements were made on the concentration of glycogen and lactate in the red muscle and white muscle at a series of known swimming speeds. Evidence is provided that red muscle alone is used at speeds below 2 bodylengths/s. The fall in concentration of red muscle glycogen was directly proportional to increased swimming speed. At speeds in excess of 2 bodylengths/s a statistically significant increase in lactate concentration occurred in the white muscle fibres. A reduction in glycogen content of the white muscle was also noted at speeds at and above the estimated mean sustained swimming speed. These results are discussed in the light of the current ideas pertaining to the division of labour between myotomal muscles in fish.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 53 , Issue 1 , February 1973 , pp. 17 - 26
Copyright
Copyright © Marine Biological Association of the United Kingdom 1973

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References

REFERENCES

Barets, A., 1961. Contributions to the study of ‘slow’ and ‘fast’ motor systems in the lateral muscle of teleosts. Archives d’anatomie microscopique et de morphologie expérimentale Paris, 50, Suppl., 1–187.Google Scholar
Barker, S. B. & Summerson, W. H., 1941. The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry, 138 (2), 535–54.Google Scholar
Beamish, F. W. H., 1968. Glycogen and lactic acid concentrations in Atlantic cod (Gadus morhua) in relation to exercise. Journal of the Fisheries Research Board of Canada, Toronto, 25, 837–51.Google Scholar
Bing, R. J., Seigel, A., Vitale, A. G., Balboni, F. A., Sparks, E., Taeschler, M., Klapper, M. & Edwards, S., 1963. Metabolic studies on the human heart in vivo. I. Carbohydrate metabolism. American Journal of Medicine, 15, 284–96.CrossRefGoogle Scholar
Black, E. C., Connor, A. R., Lam, K. K.-C. & Chiu, W.-G., 1962. Changes in glycogen, pyruvate and lactate in rainbow trout (Salmo gairdneri) during and following musculature activity. Journal of Fisheries Research Board of Canada, Toronto, 19, 409–36.CrossRefGoogle Scholar
Black, E. C., Robertson, A. C. & Parker, R. R., 1961. Some aspects of carbohydrate metabolism in fish. In Comparative physiology of Carbohydrate Metabolism in Heterothermic Animals (ed. Martin, A. W.), pp. 89–124. Seattle: University of Washington Press.Google Scholar
Black, E. C., Bosomworth, N. J. & Docherty, G. E., 1966. Combined effects of starvation and severe exercises on glycogen metabolism of rainbow trout, Salmo gairdneri. Journal of the Fisheries Research Board of Canada, Toronto, 23, 1461–3.CrossRefGoogle Scholar
Bone, Q., 1966. On the function of the two types of myotomal muscle fibre in elasmobranch fish. Journal of the Marine Biological Association of the United Kingdom, 46, 321–49.Google Scholar
Braekken, O. R., 1956. Function of the red muscle in fish. Nature, London, 178, 747–8.Google Scholar
Brett, J. R., 1967. Swimming performance of Sockeye salmon (Oncorhynchus nerka) in relation to fatigue time and temperature. Journal of the Fisheries Research Board of Canada, Toronto, 24, 1731–41.CrossRefGoogle Scholar
Carroll, N. V. R., Longley, W. & Roe, J. H., 1956. The determination of glycogen in liver and muscle by use of anthrone reagent. Journal of Biological Chemistry, 220, 583–98.Google Scholar
Finney, D. J., 1971. Probit Analysis, 3rd ed. Cambridge: Cambridge University Press.Google Scholar
Fraser, D. I., Dyer, W. J., Weinstein, H. M., Dingle, J. R. & Hines, J. A., 1966. Glycolytic metabolites and their distribution at death in the white and red muscle of cod following various degrees of ante mortem activity. Canadian Journal of Biochemistry and Physioiogy, 44, 1015–33.Google Scholar
George, J. C. & Bokdawala, F. D., 1964. Cellular organisation and fat utilization in fish muscle. Journal of Animal Morphology and Physiology, 11, 124–32.Google Scholar
Hamoir, G. Focant & Distèche, M., 1972. Proteinic criteria of differentiation of white, cardiac and various red muscles in carp. Comparative Biochemistry and Physiology, 41 B, 665–74.Google ScholarPubMed
Johnston, I. A., Frearson, N. & Goldspink, G., 1972. Myofibrillar ATPase activities of red and white myotomal muscles of marine fish. Experientia, 28 (6), 713–14.Google Scholar
Patterson, S. & Goldspink, G., 1972. The fine structure of red and white myotomal muscle in a marine teleost, the coalfish (Gadus virens). Zeitschrift für zeleforschung und mikroskopische Anatomie, 133, 463–74.CrossRefGoogle Scholar
Pritchard, A. W., Hunter, J. R. & Lasker, R., 1971. The relation between exercise and biochemical changes in red and white muscle and liver in Jack mackerel, Trachurus symmetricus. Fishery Bulletin of the National Oceanic Atmospheric Administration, 69, 379–86.Google Scholar
Rayner, M. D. & Keenan, M. J., 1967. Role of red and white muscles in the swimming of skipjack tuna. Nature, London, 214, 392–3.Google Scholar
Roberts, R. L., 1969. Spontaneous rhythms in the motor neurons of spinal dogfish (Scyliorhinus caricula). Journal of the Marine Biological Association of the United Kingdom, 49, 33–49.CrossRefGoogle Scholar
Seifter, S., Dayton, S., Novic, B. & Muntwyler, E., 1949. The estimation of glycogen with the anthrone reagent. Archives of Biochemistry, 25, 191–200.Google Scholar
Smit, H., Amelink-Koutstaal, J. M., Vijvernerg, J. & Von Vaupel-Klein, J. C., 1971. Oxygen consumption and efficiency of swimming goldfish. Comparative Biochemistry and Physiology, 39A, 1–28.Google Scholar
Stevens, E. D. & Black, E. C., 1966. The effect of intermittent exercise on carbohydrate metabolism in rainbow trout, Salmo gairdneri. Journal of the Fisheries Research Board of Canada, Toronto, 23, 471–95.Google Scholar
Tomlinson, N. & Geiger, S. E., 1962. Glycogen concentration and post mortem loss of adenosine triphosphate in fish and mammalian skeletal muscle. A review. Journal of the Fisheries Research Board of Canada, Toronto, 19, 997–1003.Google Scholar
Walker, M. G., 1970. Growth and development of the skeletal muscle fibres of the cod {Gadus morhua L.) Journal du Conseil. Conseil Permanent International pour l'Exploration de la Mer, 33, no. 2, 228–44.Google Scholar
Walker, M. G., 1971. The effects of starvation and exercise on the skeletal muscle fibres of the cod (Gadus morhua L.) and the coalfish (Gadus virens L.) respectively. Journal du Conseil. Conseil Permanent International pour l'Exploration de la Mer, 33, no. 3, 421–7.Google Scholar
Wittenberger, C., 1968. Alterations of the carbohydrate metabolism in trout, induced by effort and hypoxia. Revue roumaine de biologie, série de zoologie, 13, 131–7.Google Scholar
Wittenberger, C. & Diaciuc, I. V., 1965. Effort metabolism of lateral muscles in carp. Journal of the Fisheries Research Board of Canada, Toronto, 22, 1397–1406.Google Scholar
Wittelberger, C. & Vitca, E., 1966. Variation of the glycogen content in the lateral muscles of the carp during work performed by isolated muscles and during starvation. Studia Universotatis Babeş-Bolyai. Bucureşti, 2, 117–23.Google Scholar