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On the use of antibiotics for isolating bacteria-free cultures of marine phytoplankton organisms

Published online by Cambridge University Press:  11 May 2009

C. P. Spencer
Affiliation:
Marine Biological Station, University College of North Wales.

Extract

In the course of some studies on the kinetics of growth and the biochemical activities of a marine diatom it became desirable to obtain bacteria-free cultures. The classical method of obtaining such pure algal cultures involves either repeatedly washing single cells in sterile medium or obtaining discrete bacteria-free algal colonies by growth on a solid medium. Both these methods have been widely applied to fresh-water species by Pringsheim (1946) and others, whilst Chu (1946) has used both methods with the marine diatom Nitzschia closterium (Ehrenberg) Wm. Smith forma minutissima.

Type
Research Article
Copyright
Copyright © Marine Biological Association of the United Kingdom 1952

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