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On the nutrition and metabolism of zooplankton XIII. Further studies of steroids in Calanus

Published online by Cambridge University Press:  11 May 2009

S. C. M. O'Hara ,
S. J. Gaskellf  and
E. D. S. Corner
S. C. M. O'Hara
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
S. J. Gaskellf
Affiliation:
Tenovus Institute for Cancer Research, Cardiff
E. D. S. Corner
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
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Extract

The presence in Calanus finmarchicus of the sex hormone oestradiol-17β has been confirmed by gas chromatography-mass spectrometry (GC-MS) using the trimethylsilyl and heptafluorobutyrate derivatives.

Levels of the sex hormone testosterone have been measured by radioimmunoassay in Calanus helgolandicus collected from Plymouth Sound, and in C. finmarchicus captured off the Norwegian coast. Concentrations in C. helgolandicus varied during the year from 150 to 626 fg/animal, accounting for 2.3 to 437 x 10-7% of the lipid content and 10.8 to 64.8 x 10-6%percnt; of the total sterol fraction. The mean level in Stage V C. finmarchicus, captured during spring, was 196 fg/animal.

Testosterone was identified in C. finmarchicus by GC-MS of the tert-butyldimethylsilyl derivative.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 59 , Issue 2 , May 1979 , pp. 331 - 340
Copyright
Copyright © Marine Biological Association of the United Kingdom 1979

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References

REFERENCES

Boyd, M. J. & Alper, C., 1967. Blood (Chemical composition - Vertebrates). In The Encyclopedia of Biochemistry (ed. Williams, R. J. and Lansford, E. M. Jr), pp. 147–150. New York: Reinhold Publishing Corporation.Google Scholar
Brown, J. B., 1955. A chemical method for the determination of oestriol, oestrone and oestradiol in human urine. Biochemical Journal, 60, 185–193.CrossRefGoogle ScholarPubMed
Conover, R. J. & Corner, E. D. S., 1968. Respiration and nitrogen excretion by some marine zooplankton in relation to their life cycles. Journal of the Marine Biological Association of the United Kingdom, 48, 49–75.CrossRefGoogle Scholar
Gaskell, S. J. & Pike, A. W., 1978. Gas chromatography - high resolution mass spectrometry in the analysis of steroids in physiological fluids. In Proceedings of the Second International Symposium on Quantitative Mass Spectrometry in Life Sciences, Ghent, 1978 (ed. De Leenheer, A. P., Roncucci, R. and Van Peteghem, C.). Amsterdam: Elsevier. (In the Press.)Google Scholar
Gatten, R. R., Kilvington, C. C., Corner, E. D. S. & Sargent, J. R., 1979. Annual variations in the lipid contents of Calanus helgolandicus. In Proceedings of the 13th European Symposium on Marine Biology, Douglas, Isle of Man, 1978 (ed. Naylor, E. and Hartnoll, R. G.). Oxford: Pergamon Press.Google Scholar
Goad, L. J., 1976. The steroids of marine algae and invertebrate animals. In Biochemical and Biophysical Perspectives in Marine Biology, vol. 3 (ed. Malins, D. C. and Sargent, J. R.), pp. 213–318. London: Academic Press.Google Scholar
Gower, D. B. & Fotherby, K., 1975. Biosynthesis of the androgens and oestrogens. In Bio-chemistry of Steroid Hormones (ed. Makin, H. L. J.), pp. 77–104. Oxford: Blackwell Scientific Publications.Google Scholar
Llewelyn, D. E. H., Hillier, S. G. & Read, G. F., 1976. The use of multivariable standard curves in the radioimmunoassay of testosterone and 5α-dihydrotestosterone. Steroids, 28, 339–348.CrossRefGoogle ScholarPubMed
Means, A. R. & Hamilton, T. H., 1966. Early estrogen action: concomitant stimulations within two minutes of nuclear RNA synthesis and uptake of RNA precursor by the uterus. Proceedings of the National Academy of Science of the United States of America, 56, 1594–1598.CrossRefGoogle ScholarPubMed
O'hara, S. C. M., Corner, E. D. S. & Kilvington, C. C., 1978. On the nutrition and metabolism of zooplankton. XII. Measurements by radioimmunoassay of the levels of a steroid in Calanus. Journal of the Marine Biological Association of the United Kingdom, 58, 597–605.CrossRefGoogle Scholar
Raymont, J. E. G. & Gross, F., 1942. On the breeding and feeding of Calanus finmarchicus under laboratory conditions. Proceedings of the Royal Society of Edinburgh (B), 61, 267–287.Google Scholar
Sargent, J. R. & Mcintosh, R., 1974. Studies on the mechanism of biosynthesis of wax esters in Euchaeta norvegica. Marine Biology, 25, 271–277.CrossRefGoogle Scholar
Siekmann, L., Siekmann, A. & Breuer, H., 1978. Isotopic dilution mass spectrometry of oestriol and oestradiol-17β- an approach to definitive methods. Zeitschrift für analytische Chemie, 290, 122–123.CrossRefGoogle Scholar
Teshima, S., 1972. Studies on the sterol metabolism in marine crustaceans. Memoirs of Faculty of Fisheries, Kagoshima University, 21, 69–147.Google Scholar
Wicks, W. D., Greenman, D. L. & Kenney, F. T., 1965. Stimulation of ribonucleic acid synthesis by steroid hormones. I. Transfer ribonucleic acid. Journal of Biological Chemistry, 240, 4414–4419.CrossRefGoogle ScholarPubMed