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Metabolism of zinc in the mussel, Mytilus edulis (L.): a combined ultrastructural and biochemical study

Published online by Cambridge University Press:  11 May 2009

Stephen G. George  and
Brian J. S. Pirie
Stephen G. George
Affiliation:
Institute of Marine Biochemistry, Aberdeen, Scotland
Brian J. S. Pirie
Affiliation:
Institute of Marine Biochemistry, Aberdeen, Scotland
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Extract

The uptake, transport, storage and excretion of zinc has been studied in Mytilus edulis. Zinc accumulates in the soft tissues in proportion to its concentration in sea water whilst the concentration in the haemolymph is little above that in the environment. Uptake is via the gut, mantle and gills. The zinc is transported from the gills and gut (t½ ≈ 8 days) via the haemolymph, either as a high molecular weight complex or in the granular amoebocytes, to the kidney. Most of the body zinc is present in the granular amoebocytes (which are found in all the body tissues) or in the gut and kidney. The kidney forms the major storage organ for many trace metals, containing 30% of the body zinc and a concentration of about 1000 μg/g. Zinc is localized as insoluble granules in membranelimited vesicles occupying some 20% of the cell volume. Excretion of zinc is by defaecation, exocytosis of the kidney granules into the urine and diapedesis of the amoebocytes. A multicompartmental model for zinc metabolism which correlates the ultrastructural and kinetic data is proposed.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 60 , Issue 3 , August 1980 , pp. 575 - 590
Copyright
Copyright © Marine Biological Association of the United Kingdom 1980

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References

Boroughs, H. A., Chipman, A. & Rice, T. R., 1957. Laboratory experiments on the uptake, accumulation and loss of radionuclides by marine organisms. In Effects of Atomic Radiation on Oceanography and Fisheries, pp. 80–87. Washington: National Academy of Science. [National Research Council Publication 551.]Google Scholar
Brooks, R. R. & Rumsby, M. G., 1965. The biogeochemistry of trace element uptake by some New Zealand bivalves. Limnology and Oceanography, 10, 521–527.CrossRefGoogle Scholar
Bryan, G. W., Preston, A. & Templeton, W. L., 1966. Accumulation of radionuclides by aquatic organisms of economic importance in the United Kingdom. In Disposal of Radioactive Wastes into Seas, Oceans and Surface waters. Proceedings of a symposium, May 1966, pp. 623–667. Vienna: International Atomic Energy Agency.Google Scholar
Chipman, W. A., Rice, T. R. & Price, T. J., 1958. Uptake and accumulation of radioactive zinc by marine plankton, fish and shellfish. Fishery Bulletin. Fish and Wildlife Service. United States Department of the Interior, 58, 279–292.Google Scholar
Coombs, T. L., 1977. Uptake and storage mechanisms in marine organisms. Proceedings of the Analytical Division of the Chemical Society, 14, 218–221.Google Scholar
Coombs, T. L. & George, S. G., 1978. Mechanisms of immobilisation and detoxication of metals in marine organisms. In Physiology and Behaviour of Marine Organisms (ed. D. S., McLusky and A. J., Berry), pp. 179–187. Oxford: Pergamon Press.Google Scholar
Foulquier, L. & Baudhuin, J.-P., 1976. Étude comparée de la fixation et de la désorption du zinc 65 par un mollusqe marin (Mytilus galloprovincialis LmK) et un mollusque dulcicole (Unio requieni Michaud). Radioprotection, 11, 35–56.CrossRefGoogle Scholar
Fukai, R. & Huynh-Ngoc, L., 1975. Chemical forms of zinc in sea water - problems and experimental methods. Journal of the Oceanographical Society of Japan, 31, 179–191.CrossRefGoogle Scholar
George, S. G., Carpene, E., Coombs, T. L., Overnell, J. & Youngson, A. Y., 1979. Characterisation of cadmium-binding proteins from mussels, Mytilus edulis (L.), exposed to cadmium. Biochimica biophysica acta, 580, 225–233.CrossRefGoogle ScholarPubMed
George, S. G. & Coombs, T. L., 1977. The effects of chelating agents on the uptake of cadmium by Mytilus edulis (L.). Marine Biology, 39, 261–268.CrossRefGoogle Scholar
George, S. G., Pirie, B. J. S., Cheyne, A. R., Coombs, T. L. & Grant, P. T., 1978. Detoxication of metals by marine bivalves: an ultrastructural study of the compartmentation of copper and zinc in the oyster, Ostrea edulis. Marine Biology, 45, 147–156.CrossRefGoogle Scholar
George, S. G., Pirie, B. J. S. & Coombs, T. L., 1976. The kinetics of accumulation and excretion of ferric hydroxide in Mytilus edulis (L.) and its distribution in the tissues. Journal of Experimental Marine Biology and Ecology, 23, 71–84.CrossRefGoogle Scholar
George, S. G., Pirie, B. J. S. & Coombs, T. L., 1980. Isolation and elemental analysis of metal-rich granules from the kidney of the scallop, Pecten maximus (L.). Journal of Experimental Marine Biology and Ecology, 42, 143–156.CrossRefGoogle Scholar
George, S. G. & Pirie, B. J. S., 1979. The occurrence of cadmium in sub-cellular particles in the kidney of Cd-exposed marine mussel, Mytilus edulis: the use of electron probe microprobe analysis. Biochimica biophysica acta, 580, 234–244.CrossRefGoogle Scholar
Goldberg, E. D., 1975. The mussel watch-a first step in global marine monitoring. Marine Pollution Bulletin, 6, 111.Google Scholar
Kameda, K., Shimizu, M. & Hiyama, Y., 1968. On the uptake of 65Zn and the concentration factor for Zn in marine organisms. 1. Uptake of 65Zn in marine organisms. Journal of Radiation Research, 9, 50–62.CrossRefGoogle Scholar
Keckes, S., Ozretic, B. & Krajnovic, M., 1968. Loss of 65Zn in the mussel Mytilus galloprovincialis. Malacologia, 7,1–6.Google Scholar
Keckes, S., Ozretic, B. & Krajnovic, M., 1969. Metabolism of Zn65 in mussels (Mytilus galloprovincialis Lam.). Uptake of Zn65. Rapport et proces-verbaux des reunions. Commission Internationale pour Vexploration scientifique de la Mer Meditterranée, 19, 949–952.Google Scholar
Lowe, D. M. & Moore, M. N., 1979. The cytochemical distributions of zinc (Zn II) and iron (Fe III) in the common mussel, Mytilus edulis, and their relationship with lysosomes. Journal of the Marine Biological Association of the United Kingdom, 59, 851–858.Google Scholar
Pentreath, R. J., 1973. The accumulation from water of 65Zn, 54Mn, 56Co and 58Fe by the mussel, Mytilus edulis. Journal of the Marine Biological Association of the United Kingdom, 53, 127–143.Google Scholar
Phillips, D. J. H., 1976. The common mussel Mytilus edulis as an indicator of pollution by zinc, cadmium, lead and copper. 1. Effects of environmental variables on uptake of metals. Marine Biology, 38, 59–69.CrossRefGoogle Scholar
Phillips, D. J. H., 1977. The common mussel Mytilus edulis as an indicator of trace metals in Scandinavian waters. 1. Zinc and cadmium. Marine Biology, 43, 283–291.CrossRefGoogle Scholar
Pirie, B. J. S. & George, S. G., 1979. Ultrastructure of the heart and excretory system of Mytilus edulis (L.). Journal of the Marine Biological Association of the United Kingdom, 59, 819–829.Google Scholar
Preston, A., Jefferies, D. F., Dutton, J. W. R., Harvey, B. R. & Steele, A. K., 1972. British Isles coastal waters: the concentrations of selected heavy metals in sea water, suspended matter and biological indicators - a pilot survey. Environment Pollution, 3, 69–82.Google Scholar
Schultz-Baldes, M., 1978. Lead transport in the common mussel. In Physiology and Behaviour of Marine Organisms (ed. D. S., McLusky and A. J., Berry), pp. 211–218. Oxford: Pergamon Press.Google Scholar
Segar, D. A., Collins, J. D. & Riley, J. P., 1971. The distribution of the major minor elements in marine animals. Part II. Molluscs. Journal of the Marine Biological Association of the United Kingdom, 51, 131–136.CrossRefGoogle Scholar
Seymour, A. H. & Nelson, V. A., 1973. Decline of 65Zn in marine mussels following the shutdown of the Hanford reactors. In Radioactive Contamination of the Marine Environment, pp. 277–286. Vienna: International Atomic Energy Agency. [IAEA-SM-158/16.]Google Scholar
Tripp, M. R., 1957. Disposal by the oyster of intracardially injected red blood cells of vertebrates. Proceedings. National Shellfisheries Association, 48, 143–147.Google Scholar
Van Weers, A. W., 1973. Uptake and loss of 65Zn and 60Co by the mussel Mytilus edulis (L.). In Radioactive contamination in the Marine Environment, pp. 385–401. Vienna: International Atomic Energy Agency. [IAEA-SM-158/24.]Google Scholar
Vinogradov, A. P., 1953. The elementary composition of marine organisms. 647 pp. New Haven: Sears Foundation for Marine Research, Yale University.Google Scholar
Young, D. R. & Folsom, T. R., 1967. Loss of Zn65 from the California sea-mussel Mytilus californianus. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 133, 438–447.CrossRefGoogle Scholar