Hostname: page-component-848d4c4894-x5gtn Total loading time: 0 Render date: 2024-05-17T14:00:48.213Z Has data issue: false hasContentIssue false
  • English
  • Français

The Effect of Storage Temperature on the Enumeration of Epifluorescence-Detectable Bacterial Cells in Preserved Sea-Water Samples

Published online by Cambridge University Press:  11 May 2009

C. M. Turley  and
D. J. Hughes
C. M. Turley
Affiliation:
Plymouth Marine Laboratory, Citadel Hill, Plymouth, PL1 2PB
D. J. Hughes
Affiliation:
Plymouth Marine Laboratory, Citadel Hill, Plymouth, PL1 2PB
Get access Rights & Permissions [Opens in a new window]

Extract

Rates of decrease in epifluorescence-detectable bacteria in preserved sea-water samples changed significantly with temperature. This apparent cell loss was substantial even in preserved samples stored in a refrigerator, decreasing exponentially with time, with 56% apparent cell loss after 99 days’ storage. Storage at room temperature resulted in the greatest decrease in detectable cells, with 73% apparent cell loss after the same storage period. No significant difference was found between storage in two different containers. The apparent cell loss can be modelled in order to calculate the original cell numbers with confidence. However, models produced from one set of samples cannot be applied to the data from another.

Type
Short Communication
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 74 , Issue 1 , February 1994 , pp. 259 - 262
Copyright
Copyright © Marine Biological Association of the United Kingdom 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bjornsen, P.K., 1986. Automatic determination of bacterioplankton biomass by image analysis. Applied and Environmental Microbiology, 51, 1199–1204.CrossRefGoogle ScholarPubMed
Daley, R.J. & Hobbie, J.E., 1975. Direct counts of aquatic bacteria by a modified epifluorescence technique. Limnology and Oceanography, 20, 875–882.CrossRefGoogle Scholar
Fry, J.C., 1990. Direct methods and biomass estimation. Methods in Microbiology, 22, 41–85.CrossRefGoogle Scholar
Hobbie, J.E., Daley, R.J. & Jasper, S., 1977. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Applied and Environmental Microbiology, 33, 1225–1228.CrossRefGoogle ScholarPubMed
Jgofs, , 1990. Bacteria. In North Atlantic bloom experiment, report of the first workshop. Joint Global Ocean Flux Study (JGOFS), report no. 4, 49–56. Kiel: JGOFS-Büro, Institut für Meereskunde.Google Scholar
Jones, J.G., & Simon, B.M., 1975. An investigation of errors in direct counts of aquatic bacteria by epifluorescence microscopy, with reference to a new method for dyeing membrane filters. Journal of Applied Bacteriology, 39, 317–329.CrossRefGoogle ScholarPubMed
Kirchman, D., 1993. Statistical analysis of direct counts of microbial abundance. In Handbook of methods in aquatic microbial ecology (ed. P., Kempet al.), pp. 117–119. Boca Raton: Lewis Publishers.Google Scholar
Kirchman, D., Sigda, J., Kapuscinski, R. & Mitchell, R., 1982. Statistical analysis of the direct count method for enumerating bacteria. Applied and Environmental Microbiology, 44, 376–382.CrossRefGoogle Scholar
Porter, K.G. & Feig, Y.S., 1980. The use of DAPI for identifying and counting aquatic microflora. Limnology and Oceanography, 25, 943–948.CrossRefGoogle Scholar
Sieracki, M.E., Johnson, P.W. & Sieburth, J.McN., 1985. Detection, enumeration, and sizing of planktonic bacteria by image-analysed epifluorescence microscopy. Applied and Environmental Microbiology, 49, 799–810.CrossRefGoogle Scholar
Turley, C.M., 1993. Direct estimates of bacterial numbers in sea-water samples without incurring cell loss due to sample storage. In Handbook of methods in aquatic microbial ecology (ed. P., Kempet al.), pp. 143–147. Boca Raton: Lewis Publishers.Google Scholar
Turley, C.M. & Hughes, D.J., 1992. Effects of storage on direct estimates of bacterial numbers of preserved sea-water samples. Deep-Sea Research, 39, 375–394.CrossRefGoogle Scholar
Watson, S.W., Novitsky, T.J., Quinby, H.L. & Valois, F.W., 1977. Determination of bacterial number and biomass in the marine environment. Applied and Environmental Microbiology, 33, 940–946.CrossRefGoogle ScholarPubMed
Zimmermann, R. & Mayer-Reil, L.A., 1974. A new method for fluorescent staining of bacterial populations on membrane filters. Kieler Meeresforschungen, 30, 24–27.Google Scholar