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Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling

Published online by Cambridge University Press:  09 June 2016

Thomas J. Ashton ,
Meriem Kayoueche-Reeve ,
Andrew J. Blight ,
Jon Moore  and
David M. Paterson
Thomas J. Ashton*
Affiliation:
Xelect Ltd, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK
Meriem Kayoueche-Reeve
Affiliation:
The Sullom Voe Association Ltd, Shetland Oil Terminal Environmental Advisory Group (SOTEAG) Office, School of Biology, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK
Andrew J. Blight
Affiliation:
Sediment Ecology Research Group, School of Biology, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK
Jon Moore
Affiliation:
Aquatic Survey & Monitoring Ltd, Cosheston, Pembroke Dock, Pembrokeshire SA72 4UN, Wales, UK
David M. Paterson
Affiliation:
Sediment Ecology Research Group, School of Biology, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK
*
Correspondence should be addressed to: T.J. Ashton, Xelect Ltd, Scottish Oceans Institute, East Sands, St Andrews KY16 8LB, UK email: tom.ashton@xelect.co.uk
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Abstract

Accurate discrimination of two morphologically similar species of Patella limpets has been facilitated by using qPCR amplification of species-specific mitochondrial genomic regions. Cost-effective and non-destructive sampling is achieved using a mucus swab and simple sample lysis and dilution to create a PCR template. Results show 100% concurrence with dissection and microscopic analysis, and the technique has been employed successfully in field studies. The use of highly sensitive DNA barcoding techniques such as this hold great potential for improving previously challenging field assessments of species abundance.

Keywords

PatellalimpetsDNA barcodingmucusspecies determination
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 7 , November 2017 , pp. 1479 - 1482
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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