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Effect of infection by the protistan parasite Marteilia refringens on the enzyme activity and energy reserves of oyster Ostrea stentina (Payraudeau, 1826) in Tunisia

Published online by Cambridge University Press:  03 October 2016

Refka Elgharsalli ,
Catherine Seguineau ,
Isabelle Arzul ,
Nejla Aloui-Bejaoui ,
Claudie Quere  and
Jeanne Moal
Refka Elgharsalli*
Affiliation:
Institut National Agronomique de Tunisie, 43, Avenue Charles Nicolle, 1082 Tunis, Tunisia
Catherine Seguineau
Affiliation:
IFREMER, Laboratoire de Physiologie des Invertébrés, Centre de Brest, 29280 Plouzané, France
Isabelle Arzul
Affiliation:
IFREMER, Laboratoire de Génétique et Pathologie, avenue de Mus de Loup, 17390 La, Tremblade, France
Nejla Aloui-Bejaoui
Affiliation:
Institut National Agronomique de Tunisie, 43, Avenue Charles Nicolle, 1082 Tunis, Tunisia
Claudie Quere
Affiliation:
IFREMER, Laboratoire de Physiologie des Invertébrés, Centre de Brest, 29280 Plouzané, France
Jeanne Moal
Affiliation:
IFREMER, Laboratoire de Physiologie des Invertébrés, Centre de Brest, 29280 Plouzané, France
*
Correspondence should be addressed to: R. Elgharsalli, Institut National Agronomique de Tunisie, 43, Avenue Charles Nicolle, 1082 Tunis, Tunisia email: refkagr@yahoo.fr
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Abstract

This is the first study of the metabolic enzyme activity and energy state of the dwarf oyster Ostrea stentina (Payraudeau, 1826) in Tunisia and in the Mediterranean region. The main purpose of this study was to examine the modulation of the physiological status of the oyster O. stentina depending on the season and the presence of parasite Marteilia refringens. The prevalence of bonamiosis and marteiliosis were established by PCR. Bonamia exitiosa was detected only in 2.91% of oysters while the prevalence of M. refringens was 100% (30 Ind./30) in February and 93.93% (28 Ind./30) in March. A 42–87% mortality rate was reported during the study period. Biochemical analyses were carried out to evaluate the management of the energy resources in regard to the biochemical changes of lipids and carbohydrates. The enzyme activity of pyruvate kinase (PK), citrate synthase (CS) and cytochrome c oxidase (CCO) were measured and the mean level of enzyme activity was respectively 20.31 mU mg−1 protein; 12.06 mU mg−1 protein and 3.59 mU mg−1 protein. Carbohydrate contents of O. stentina were very low all year round with an average of 15.18% in dry weight, and lipid contents remain similar (11.77% in dry weight) compared with the values reported for most other temperate bivalves. Enzyme activity significantly decreased over time (P < 0.001). The energy resources of the oyster O. stentina wherein much of the energy was devoted to reproduction seem to affect the defence system.

Keywords

OystersMarteilia refringensmortalityhost-parasite interactionenergy reservesenzyme activity
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 98 , Special Issue 1: Special Section: European Marine Biology Symposium Papers 2016 , February 2018 , pp. 161 - 170
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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