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Upconversion rare earth nanoparticles functionalized with folic acid for bioimaging of MCF-7 breast cancer cells

  • Dalia Chávez-García (a1), Karla Juárez-Moreno (a2), Cristian H. Campos (a3), Joel B. Alderete (a3) and Gustavo A. Hirata (a4)...


Luminescent biolabels are being eagerly investigated as a means of detecting cancer cells by bioimaging. Upconversion nanoparticles are a promising option to be used as biolabels for cancer cell detection. This process uses a near infrared beam (NIR λ = 980 nm) as the excitation source to upconvert the energy into light in the visible region. The present study, used Y2O3:Yb3+, Er3+ (1%, 10% mol) and Gd2O3:Yb3+, Er3+ (1%, 10% mol) capable of emitting red photons of λ = 660 nm. The nanoparticles were previously functionalized with aminosilanes and folic acid (UCNP-NH2-FA). Folic acid binds to the folate receptor on the surface of MCF-7 breast cancer cells, and this binding promotes internalization of the UCNPs via endocytosis. The UCNPs were characterized by TEM, EDS, and Fourier transform infrared. Cytotoxicity was also analyzed using the MTT (methy-134 thiazolyltetrazolium) colorimetric assay. The UCNPs-NH2-FA was noncytotoxic to the studied cancer cells and they were clearly localizable within the cell cytoplasm via confocal microscope.


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1. Blasse, G. and Grabmaier, B.: Luminescent Materials, Telos (Springer-Verlag, Berlin, 1994).
2. Da Costa, M., Doughan, S., Han, Y., and Krull, U.: Lanthanide upconversion nanoparticles and applications in bioassays and bioimaging: A review. Anal. Chim. Acta 832, 1 (2014).
3. Chávez, D., Contreras, O., and Hirata, G.: Synthesis and upconversion luminescence of nanoparticles Y2O3 and Gd2O3 co-doped with Yb3+ and Er3+ . Nanomater. Nanotechnol. 6, 7 (2016).
4. Zhang, F.: Photon Upconversion Nanomaterials, Nanostructure Science and Technology (Springer-Verlag, Berlin Heidelberg, 2015).
5. Kong, W., Shan, J., and Ju, Y.: Flame synthesis and effects of host materials on Yb3+/Er3+ co-doped upconversion nanophosphors. Mater. Lett. 64, 668 (2010).
6. Chávez, D., Juárez-Moreno, K., and Hirata, G.: Aminosilane functionalization and cytotoxicity effects of upconversion nanoparticles Y2O3 and Gd2O3 co-doped with Yb3+ and Er3+ . Nano Biomed. 3, 1 (2016).
7. Lu, Y., Sega, E., Leamon, C-P., and Low, P-S.: Folate receptor-targeted immunotherapy of cancer: Mechanism and therapeutic potential. Adv. Drug Delivery Rev. 56, 1161 (2000).
8. Soule, H-D., Vazquez, J., Long, A., Albert, S., and Brennan, M.: A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51, 1409 (1973).
9. Hemmer, E., Yamano, T., Kishimoto, H., Venkatachalam, N., Hyodoand, H., and Soga, K.: Cytotoxic aspects of gadolinium oxide nanostructures for up-conversion and NIR bioimaging. Acta Biomater. 9, 4734 (2012).
10. Sudimack, J. and Lee, R-J.: Targeted drug delivery via the folate receptor. Adv. Drug Delivery Rev. 41, 147 (2000).
11. Stöber, W., Fink, A., and Bohn, E.: Controlled growth of monodisperse silica spheres in the micron size range. J. Colloid Interface Sci. 26, 62 (1968).
12. Sounderya, N. and Zhang, Y.: Upconversion nanoparticles for imaging cells. Proceedings 23, 741 (2009).
13. Martínez-Carpio, P-A.: Constitutive and regulated secretion of epidermal growth factor and transforming growth factor-beta1 in MDA-MB-231 breast cancer cell line in 11-day cultures. Cell. Signalling 11, 753 (1999).
14. Sanchez-Sanchez, L., Tapia-Moreno, A., Juarez-Moreno, K., Patterson, D., Cadena-Nava, R., Douglas, T., and Vazquez-Duhalt, R.: Design of a VLP nanovehicle for CYP450 enzymatic activity delivery. J. Nanobiotechnol. 13, 66 (2015).
15. Chatterjee, D-K., Rufaihah, A-J., and Zhang, Y.: Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals. Biomaterials 29, 937 (2007).
16. Lippman, M-E. and Bolan, G.: Oestrogen-responsive human breast cancer in long-term tissue culture. Nature 256, 592 (1975).
17. Horwitz, K-B., Costlow, M-E., and McGuire, W-L.: MCF-7: A human breast cancer cell line with estrogen, androgen, progesterone and glucocorticoid receptors. Steroids 26, 785 (1975).
18. Vetrone, F., Boyer, J-C., and Capobianco, J-A.: Effect of Yb3+ codoping on the upconversion emission in nanocrystalline Y2O3:Er3+ . J. Phys. Chem. 107, 1107 (2003).
19. Hirai, T. and Orikoshi, T.: Preparation of Gd2O3:Yb, Er and Gd2O2S:Yb, Er infrared to visible conversion phosphor ultradine particles using an emulsion liquid membrane system. J. Colloid Interface Sci. 269, 103 (2004).


Upconversion rare earth nanoparticles functionalized with folic acid for bioimaging of MCF-7 breast cancer cells

  • Dalia Chávez-García (a1), Karla Juárez-Moreno (a2), Cristian H. Campos (a3), Joel B. Alderete (a3) and Gustavo A. Hirata (a4)...


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