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Biosynthesis and adhesion of gold nanoparticles for breast cancer detection and treatment

Published online by Cambridge University Press:  12 November 2012

E. Hampp ,
R. Botah ,
O.S. Odusanya ,
N. Anuku ,
K.A. Malatesta  and
W.O. Soboyejo
E. Hampp
Affiliation:
Princeton Institute for Science and Technology of Materials (PRISM), Princeton University, Princeton, New Jersey 08544; and Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544
R. Botah
Affiliation:
Department of Materials Science and Engineering, African University of Science and Technology, Abuja, Nigeria
O.S. Odusanya
Affiliation:
Sheda Science and Technology Complex, Gwagwalada, Federal Capital Territory, Abuja, Nigeria
N. Anuku
Affiliation:
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544; and Bronx Community College, Bronx, New York 10453
K.A. Malatesta
Affiliation:
Princeton Institute for Science and Technology of Materials (PRISM), Princeton University, Princeton, New Jersey 08544; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544; and Department of Materials Science and Engineering, African University of Science and Technology, Abuja, Nigeria
W.O. Soboyejo*
Affiliation:
Princeton Institute for Science and Technology of Materials (PRISM), Princeton University, Princeton, New Jersey 08544; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544; and Department of Materials Science and Engineering, African University of Science and Technology, Abuja, Nigeria
*
a)Address all correspondence to this author. e-mail: soboyejo@princeton.edu
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Abstract

Gold nanoparticles (AuNPs) were biosynthesized using Bacillus megaterium, a common soil bacterium. Transmission electron microscopy images revealed that well-developed, spherical, homogeneous nanoparticles are formed extracellularly in reactions containing aqueous chloroaurate ions and conditioned medium at pH 4. Atomic force microscopy measurements showed that adhesion forces between biosynthesized AuNPs and breast cancer cells were almost six times greater than adhesion forces between biosynthesized AuNPs and normal breast cells. Furthermore, adhesion forces of biosynthesized AuNPs to breast cancer cells were three times greater than adhesion forces between chemically synthesized AuNPs and the same breast cancer cells. Finally, adhesion forces between biosynthesized AuNPs conjugated to breast-specific antibodies (AuNP-Ab conjugates), and breast cancer cells were almost five times greater than adhesion forces between unconjugated AuNPs and breast cancer cells. The implications of the results are discussed for the development of nanostructures for the targeted detection and treatment of breast cancer.

Type
Articles
Information
Journal of Materials Research , Volume 27 , Issue 22 , 22 November 2012 , pp. 2891 - 2901
Copyright
Copyright © Materials Research Society 2012

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