Skip to main content Accessibility help
×
Home

Protective effect of melatonin against gentamicin ototoxicity

  • L-F Ye (a1), Z-Z Tao (a2), Q-Q Hua (a2), B-K Xiao (a2), X-H Zhou (a1), J Li (a1) and Y-L Yuan (a3)...

Abstract

Objective:

To research the protective effect of melatonin against gentamicin ototoxicity.

Methods:

Guinea pigs were randomly divided into four groups. The first group received intramuscular gentamicin (120 mg/kg body weight/day) for 17 days. Over the same time period, a second group simultaneously received intramuscular gentamicin (120 mg/kg body weight/day) plus (on the other side) intramuscular melatonin (0.3 ml kg body weight/day). Two groups of controls were treated for 17 days with either intramuscular melatonin or intramuscular saline. After the 17 days, each animal underwent distortion product otoacoustic emission testing (both ears). The guinea pigs were sacrificed by decapitation just after the final injection. Their cochleae were used to produce a tissue section, surface preparation and scanning electron microscope preparation.

Results:

Distortion product otoacoustic emission testing indicated gentamicin-induced hearing loss at 3, 4, 6 and 8 kHz in gentamicin-treated animals. Animals receiving melatonin co-therapy had significantly attenuated hearing loss and their cochleae showed lower rates of outer hair cell loss (comparing the same cochlear turns), compared with gentamicin-treated animals (p < 0.01).

Conclusion:

These findings confirm the occurrence of outer hair cell loss after gentamicin treatment, and the attenuation of such loss following simultaneous melatonin injection, using the method of morphological evaluation. These results suggest that melatonin protects against gentamicin ototoxicity by interfering with cytotoxic mechanisms.

Copyright

Corresponding author

Address for correspondence: Dr ZeZhang Tao, Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, PR China. Fax: 862767812892 E-mail: Yelinfeng69@163.com

References

Hide All
1 Williams, SE, Zenner, HP, Schacht, J. Three molecular steps of aminoglycoside ototoxicity demonstrated in outer hair cells. Hear Res 1987;30:1118
2 Schacht, J. Antioxidant therapy attenuates aminoglycoside induced hearing loss. Ann N Y Acad Sci 1999;884:125–30
3 Sha, SH, Schacht, J. Prevention of aminoglycoside induced hearing loss. Keio J Med 1997;46:115–19
4 Priuska, EM, Schacht, J. Formation of free radicals by gentamicin and iron and evidence for an iron/gentamicin complex. Biochemical Pharmacology 1995;50:1749–52
5 Roland, PS. New developments in our understanding of ototoxicity. Ear Nose Throat J 2004;83:1516
6 Reiter, RJ, Guerrero, JM, Garcia, JJ, Acuna-Castroviejo, D. Reactive oxygen intemediates, molecular damage, and aging, Relation to melatonin. Ann N Y Sci 1998;854:410–24
7 Ye, L, Xiao, B, Hua, Q, Tao, Z, Huang, Z, Zhou, X. Research on DPOAE of guinea pigs under awake and anesthetic conditions [in Chinese]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2004;21:625–7
8 Garetz, SL, Altschuler, RA, Schacht, J. Attenuation of gentamicin ototoxicity by glutathione in the guinea pig in vivo. Hear Res 1994;77:81–7
9 Kalkandelen, S, Selimoglu, E, Erdogan, F, Ucuncu, H, Alats, E. Comparative cochlear toxicities of streptomycin, gentamicin, amikacin and netilmicin in guinea-pigs. J Int Med Res 2002;30:406–12
10 Lesniak, W, Pecoraro, VL, Schacht, J. Ternary complexes of gentamicin with iron and lipid catalyze formation of reactive oxygen species. Chem Res Toxicol 2005;18:357–64
11 Sha, SH, Schacht, J. Formation of reactive oxygen species following bioactivation of gentamicin. Free Radical Biol Med 1999;26:341–7
12 Lesniak, W, Harris, WR, Yudenfreud-Kraviz, J, Schacht, J, Pecoraro, VL. Solution chemistry of copper(II)-gentamicin complexes. Relevance to metal-related aminoglycoside toxicity. Inorg Chem 2003;42:1420–9
13 Thalmann, R, Kusakari, I, Miyoshi, T. Dysfunction of energy releasing and consuming processes of the cochlea. Laryngoscope 1973;83:1690–712
14 Clerici, WJ, Hensley, K, Dimartino, DL, Butterfield, DA. Direct detection of ototoxicant-induced reactive oxygen species generation in cochlear explants. Hear Res 1996;98:116–24
15 Tan, DX, Manchester, LC, Reiter, RJ, Qi, WB, Karbownik, M, Calvo, JR. Significance of melatonin in antioxidative defence system: reactions and products. Biol Signals Recept 2000;9:137–59
16 Reiter, RJ, Tan, DX, Cabrera, J, D'Arpa, D, Sainz, RM, Mayo, JC, Ramos, S. The oxidant/antioxidant network: role of melatonin. Biol Signals Recept 1999;8:5663

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed