Skip to main content Accessibility help
×
Home

Pomegranate extract: a potential protector against aminoglycoside ototoxicity

  • V Kahya (a1), B Ozucer (a1), R Dogan (a2), A Meric (a1), M Yuksel (a3), O Gedikli (a4) and O Ozturan (a1)...

Abstract

Objective:

To investigate the effectiveness of pomegranate extract as protection against aminoglycoside ototoxicity.

Design:

Prospective, randomised, controlled, experimental study.

Subjects:

Eighteen Wistar albino rats were randomly allocated to 5 days of either: saline injections; gentamicin injections; or pomegranate extract (100 µl/day via gavage) plus gentamicin injections. Distortion product otoacoustic emissions were tested before treatment and on day 3. After treatment, reactive oxygen species levels were measured in each rat's right cochlea and right kidney via chemiluminescence.

Results:

Baseline emission amplitudes were similar. Post-treatment emissions differed significantly in the two treatment groups (p < 0.001). Cochlear reactive oxygen species levels were significantly higher in the gentamicin group (mean ± standard deviation, 316.6 ± 36.5 relative light units per mg) than the gentamicin plus pomegranate extract group (240 ± 24.6 relative light units per mg) (p = 0.004); control group levels were 119.1 ± 10.3 relative light units per mg. Renal reactive oxygen species levels were similar for the control and gentamicin plus pomegranate extract groups (p = 0.59) but much higher in the gentamicin group (p = 0.004).

Conclusion:

Concurrent systemic pomegranate extract administration reduced reactive oxygen species level increases and otoacoustic emission changes, following aminoglycoside injection.

Copyright

Corresponding author

Address for correspondence: Dr Berke Ozucer, Department of Otorhinolaryngology, Medical Faculty, Bezmialem Vakif University, Fatih, Istanbul, Turkey Fax: +90 2125332326 E-mail: berkeozucer@gmail.com

References

Hide All
1Schacht, J. Aminoglycoside ototoxicity: prevention in sight? Otolaryngol Head Neck Surg 1998;118:674–7
2Forge, A, Schacht, J. Aminoglycoside antibiotics. Audiol Neurootol 2000;5:322
3Kopke, R, Allen, KA, Henderson, D, Hoffer, M, Frenz, D, Van de Water, T. A radical demise. Toxins and trauma share common pathways in hair cell death. Ann N Y Acad Sci 1999;884:171–91
4Sha, SH, Schacht, J. Stimulation of free radical formation by aminoglycoside antibiotics. Hear Res 1999;128:112–18
5Lopez-Gonzalez, MA, Delgado, F, Lucas, M. Aminoglycosides activate oxygen metabolites production in the cochlea of mature and developing rats. Hear Res 1999;136:165–8
6Lopez-Gonzalez, MA, Guerrero, JM, Rojas, F, Delgado, F. Ototoxicity caused by gentamicin is ameliorated by melatonin and other antioxidants. J Pineal Res 2000;28:7380
7Sinswat, P, Wu, WJ, Sha, SH, Schacht, J. Protection from ototoxicity of intraperitoneal gentamicin in guinea pig. Kidney Int 2000;58:2525–32
8Nordang, L, Anniko, M. Nitro-L-arginine methyl ester: a potential protector against gentamicin ototoxicity. Acta Otolaryngol 2005;125:1033–8
9de Nigris, F, Williams-Ignarro, S, Sica, V, Lerman, LO, D'Armiento, FP, Byrns, RE et al. Effects of a pomegranate fruit extract rich in punicalagin on oxidation-sensitive genes and eNOS activity at sites of perturbed shear stress and atherogenesis. Cardiovasc Res 2007;73:414–23
10Tugcu, V, Kemahli, E, Ozbek, E, Arinci, YV, Uhri, M, Erturkuner, P et al. Protective effect of a potent antioxidant, pomegranate extract, in the kidney of rats with nephrolithiasis induced by ethylene glycol. J Endourol 2008;22:2723–31
11Aviram, M, Dornfeld, L. Pomegranate extract consumption inhibits serum angiotensin converting enzyme activity and reduces systolic blood pressure. Atherosclerosis 2001;158:195–8
12Lopez-Gonzalez, MA, Guerrero, JM, Torronteras, R, Osuna, C, Delgado, F. Ototoxicity caused by aminoglycosides is ameliorated by melatonin without interfering with the antibiotic capacity of the drugs. J Pineal Res 2000;28:2633
13Polat, Ş, Öztürk, Ö, Üneri, C, Yüksel, M, Haklar, G, Bozkurt, S et al. Determination of reactive oxygen species in myringotomized tympanic membranes: effect of vitamin E treatment. Laryngoscope 2004;114:720–5
14Haklar, G, Sayin-Özveri, E, Yüksel, M, Aktan, , Yalçın, AS. Different kinds of reactive oxygen and nitrogen species were detected in colon and breast tumors. Cancer Lett 2001;165:219–24
15Nakashima, T, Teranishi, M, Hibi, T, Kobayashi, M, Umemura, M. Vestibular and cochlear toxicity of aminoglycosides – a review. Acta Otolaryngol 2000;120:904–11
16Halsey, K, Skjönsberg, A, Ulfendahl, M, Dolan, DF. Efferent-mediated adaptation of the DPOAE as a predictor of aminoglycoside toxicity. Hear Res 2005;201:99108
17Priuska, E, Schacht, J. Formation of free radicals by gentamicin and iron and evidence for an iron/gentamicin complex. Biochem Pharmacol 1995;50:1749–52
18Sha, SH, Schacht, J. Formation of reactive oxygen species following bioactivation of gentamicin. Free Rad Biol Med 1999;26:341–7
19Sha, SH, Zajic, G, Epstein, CJ, Schacht, J. Overexpression of copper/zinc-superoxide dismutase protects from kanamycin-induced hearing loss. Audiol Neurootol 2001;6:117–23
20Hirose, K, Hockenbery, DM, Rubel, EW. Reactive oxygen species in chick hair cells after gentamicin exposure in vitro. Hear Res 1997;104:114
21Clerici, WJ, DiMartino, DL, Prasad, MR. Direct effects of reactive oxygen species on cochlear outer hair cell shape in vitro. Hear Res 1995;84:3040
22Aviram, M, Dornfeld, L, Kaplan, M, Coleman, R, Gaitini, D, Nitecki, S et al. Pomegranate juice flavonoids inhibit low-density lipoprotein oxidation and cardiovascular diseases: studies in atherosclerotic mice and in humans. Drugs Exp Clin Res 2002;28:4962
23Tsuda, T, Horio, F, Osawa, T. Cyanidin 3-O-beta-D-glucoside suppresses nitric oxide production during a zymosan treatment in rats. J Nutr Sci Vitaminol (Tokyo) 2002;48:305–10
24Maniu, A, Perde-Schrepler, M, Cosgarea, M. Protective effect of L-N-acetylcysteine against gentamycin ototoxicity in the organ cultures of the rat cochlea. Rom J Morphol Embryol 2011;52:159–64
25Liu, HY, Chi, FL, Gao, WY. Taurine attenuates aminoglycoside ototoxicity by inhibiting inducible nitric oxide synthase expression in the cochlea. Neuroreport 2008;19:117–20
26Walker, PD, Shah, SVL. Evidence suggesting a role for hydroxyl radical in gentamicin-induced acute renal failure in rats. J Clin Invest 1988;81:334–41

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed