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Microenvironment of macula flava in the human vocal fold as a stem cell niche

  • K Sato (a1), S Chitose (a1), T Kurita (a1) and H Umeno (a1)

Abstract

Background:

There is growing evidence that the cells in the maculae flavae are tissue stem cells of the human vocal fold mucosa, and that the maculae flavae are a candidate for a stem cell niche. The role of microenvironment in the maculae flavae of the human vocal fold mucosa was investigated.

Method:

Anterior maculae flavae from six surgical specimens were cultured in a mesenchymal stem cell growth medium or a Dulbecco's modified Eagle's medium.

Results:

Using mesenchymal stem cell growth medium, the subcultured cells formed a colony-forming unit, and cell division reflected asymmetric self-renewal. This indicates that these cells are mesenchymal stem cells or stromal stem cells in the bone marrow. Using Dulbecco's modified Eagle's medium, the subcultured cells showed symmetric cell division without a colony-forming unit.

Conclusion:

A proper microenvironment in the maculae flavae of the human vocal fold mucosa is necessary to be effective as a stem cell niche that maintains the stemness of the contained tissue stem cells.

Copyright

Corresponding author

Address for correspondence: Dr Kiminori Sato, Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan Fax: +81 942 37 1200 E-mail: kimisato@oct-net.ne.jp

Footnotes

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Presented at the 136th Annual Meeting of the American Laryngological Association, 22–23 April 2015, Boston, Massachusetts, USA.

Footnotes

References

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1Hirano, M, Sato, K. Histological Color Atlas of the Human Larynx. San Diego: Singular Publishing Group, 1993
2Sato, K, Hirano, M, Nakashima, T. Stellate cells in the human vocal fold. Ann Otol Rhinol Laryngol 2001;110:319–25
3Sato, K, Hirano, M, Nakashima, T. Vitamin A-storing stellate cells in the human vocal fold. Acta Otolaryngol 2003;123:106–10
4Sato, K, Umeno, H, Nakashima, T. Functional histology of the macula flava in the human vocal fold. Part 1: Its roles in the adult vocal fold. Folia Phoniatr Logop 2010;62:178–84
5Sato, K, Hirano, M, Nakashima, T. Fine structure of the human newborn and infant vocal fold mucosae. Ann Otol Rhinol Laryngol 2001;110:417–24
6Sato, K, Nakashima, T. Vitamine A-storing stellate cells in the human newborn vocal fold. Ann Otol Rhinol Laryngol 2005;114:517–24
7Sato, K, Umeno, H, Nakashima, T. Functional histology of the macula flava in the human vocal fold. Part 2: Its roles in the growth and development of the vocal fold. Folia Phoniatr Logop 2010;62:263–70
8Sato, K, Hirano, M, Nakashima, T. Age-related changes in vitamine A-storing stellate cells of human vocal fold. Ann Otol Rhinol Laryngol 2004;113:108–12
9Sato, K, Umeno, H, Nakashima, T. Vocal fold stellate cells in the human macula flava and the diffuse stellate cell system. Ann Otol Rhinol Laryngol 2012;121:51–6
10Sato, K, Umeno, T, Nakashima, T. Vocal fold stem cells and their niche in the human vocal fold. Ann Otol Rhinol Laryngol 2012;121:798803
11Xie, T, Li, L. Stem cell niche: structure and function. Annu Rev Cell Dev Biol 2005;21:605–31
12Nilsson, SK, Haylock, DN. The role of hyaluronic acid in hemopoietic stem cell biology. Regen Med 2006;1:437–45
13Preston, M, Sherman, LS. Neural stem cell niches: roles for the hyaluronan-based extracellular matrix. Front Biosci (Schol Ed) 2011;3:1165–79
14Toole, BP. Proteoglycans and hyaluronan in morphogenesis and differentiation. In: Hay, E, ed. Cell Biology of Extracellular Matrix, 2nd edn.New York: Plenum Press, 1991;305–41
15Becker, WM, Kleinsmith, LJ, Hardin, J. The cell cycle, DNA replication, and mitosis. In: The World of the Cell, 6th edn.San Francisco: Pearson Education, publishing as Benjamin Cummings, 2006;554–71
16Kurita, T, Sato, K, Chitose, S, Fukahori, M, Sueyoshi, S, Umeno, H. Origin of vocal fold stellate cells in the human macula flava. Ann Otol Rhinol Laryngol 2015;124:698705
17Friedenstein, AJ, Deriglasova, UF, Kulagina, NN, Panasuk, AF, Rudakowa, SF, Luria, EA et al. Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol 1974;2:8392
18Pittenger, MF, Mackay, AM, Beck, SC, Jaiswal, RK, Douglas, R, Mosca, JD et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143–7
19Thomson, JA, Itskovitz-Eldor, J, Shapiro, SS, Waknitz, MA, Swiergiel, JJ, Marshall, VS et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145–7
20Takahashi, K, Tanabe, K, Ohnuki, M, Narita, M, Ichisaka, T, Tomoda, K et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007;131:861–72
21Suzuki, A, Zheng, YW, Kondo, R, Kusakabe, M, Takada, Y, Fukao, K et al. Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver. Hepatology 2000;32:1230–9
22Osafune, K, Takasato, M, Kispert, A, Asashima, M, Nishinakamura, R. Identification of multipotent progenitors in the embryonic mouse kidney by a novel colony-forming assay. Development 2006;133:151–61
23Deasy, BM. Asymmetric behavior in stem cells. In: Rajasekhar, VK, Vemuri, MC, ed. Regulatory Networks in Stem Cells. New York: Humana Press, 2009;1325

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