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Long-term observation of subcutaneous tissue reaction to synthetic auditory ossicle (Apaceram®) in rats

  • Dong-Jun Li (a1), Katsuichiro Ohsaki (a1), Kunio Ii (a2), Qing Ye (a2), Yoko Nobuto (a1), Satoru Tenshin (a3) and Teruko Takano-Yamamoto (a3)...


The present study evaluates histological characteristics of the soft tissue response to long-term implantation of Apaceram™ discs composed of dense hydroxyapatite in rats. Discs were implanted into the subcutaneous tissue of 76 rats for six to 20 months. Decalcified histological sections stained with haematoxylin and eosin (H & E) and Mallory's azan were examined. Different cell types surrounding implants were counted. The greatest proportion of macrophages was found at six months (13.5 per cent). This proportion gradually decreased to four per cent at 20 months. Small numbers of lymphocytes and foreign body giant cells were observed in every group, but neither neutrophils nor osteogenesis were observed in any specimens. Results of the present study and previous related studies indicate that despite reappearance of a small number of macrophages six months after implantation, Apaceram™ is useful for reconstructive surgery.


Corresponding author

Address for correspondence: Katsuichiro Ohsaki, M.D., Ph.D., Division of Clinical Otology, University Hospital, School of Medicine, The University of Tokushima, Kuramoto 2-50-1, Tokushima 770, Japan. Fax: +81-886-33-7208


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This paper was presented 17–19 October 1996 at the conference of the Otological Society of Japan in Tokyo, organized by the Department of Otolaryngology, School of Medicine, Keio University, Tokyo 160, Japan.



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Abe, E., Ishimi, Y., Tanaka, H., Miyaura, C., Nagasawa, H., Hayashi, T., Suda, T. (1987) The relationship between fusion and proliferation in mouse alveolar macrophages. Endocrinology 121: 271277.
Anderson, J. M., Miller, K. M. (1984) Biomaterial biocompatibility and the macrophage. Biomaterials 5: 510.
Behling, C. A., Spector, M. (1986) Quantitative characterization of cells at the interface of long-term implants of selected polymers. Journal of Biomedical Materials Research 20: 653666.
Buskinsky, D. A., Sessler, N. E., Glena, T. E., Featherstone, J. D. B. (1994) Proton-induced physicochemical calcium release from ceramic apatite disks. Journal of Bone and Mineral Research 9: 213220.
Cui, P. C., Ohsaki, K., Ii, K., Tenshin, S., Kawata, T. (1995) Subcutaneous tissue reaction to synthetic auditory ossicle (Apaceram™) in rats. Journal of Laryngology and Otology 109: 1418.
Daculsi, G. (1988) Biological properties of calcium phosphate biomaterials. In Transplants and Implants in Otology (Babighian, G., Valdman, J. E., eds.), Kugler and Ghedini Publ., Amsterdam, pp 227229.
den Hollander, W., Patka, P., Klein, C. P. A. T., Heidendal, G. A. K. (1991) Macroporous calcium phosphate ceramics for bone substitution: a tracer study on biodegradation with 45Ca tracer. Biomaterials 12: 569573.
Goldenberg, R. A. (1992) Hydroxyapatite ossicular replacement prostheses: a four year experience. Otolaryngology – Head and Neck Surgery 106: 261269.
Grote, J. J., van Blitterswijk, C. A., Kuijpers, W. (1986) Hydroxyapatite ceramics as middle ear implant material: animal experimental results. Annals of Otology, Rhinology and Laryngology 95 (Suppl. 123): 15.
Grote, J. J. (1990) Reconstruction of the middle ear with Hydroxyapatite implants: long-term results. Annals of Otology, Rhinology and Laryngology 99: 1216.
Jacob-LaBarre, J. T., Assouline, M., Byrd, T., McDonald, M. (1994) Synthetic scleral reinforcement materials: I. Development and in vivo tissue biocompatibility response. Journal of Biomedical Materials Research 28: 699712.
Kao, W. J., Zhao, Q. H., Hiltner, A., Anderson, J. M. (1994) Theoretical analysis of in vivo macrophage adhesion and foreign body giant cell formation on polydimethylsiloxane, low density polyethylene, and polyetherurethanes. Journal of Biomedical Materials Research 28: 7379.
Moore, W. J. (1962) Physical chemistry, 3rd Edition. Prentice-Hall Inc., New Jersey, pp 645677.
Ohsaki, K., Shibata, A., Wang, K. Q., Ohe, M., Goto, S., Kimura, N., Yamamoto, A., Yamashita, S. (1993) Processes of de- and remineralization in vivo and in vitro studied using a synthetic ossicular chain. In Cholesteatoma and Mastoid Surgery (Nakano, Y., ed.), Kugler Publications, Amsterdam/New York, pp 615621.
Ohsaki, K., Shibata, A., Yamashita, S., Oe, M., Wang, K. Q., Cui, P. C., Ye, Q. (1995) Demonstrations of de- and remineralization mechanism as revealed in synthetic auditory ossicle (Apaceram™) of rats by Laser-Raman spectrometry. Cellular and Molecular Biology 41: 11551167.
Salthouse, T. N. (1984) Some aspects of macrophage behavior at the implant interface. Journal of Biomedical Materials Research 18: 395401.
Smetana, K. Jr (1987) Multinucleate foreign-body giant cell formation. Experimental and Molecular Pathology 46: 258265.
Thomann, J. M., Voegel, J. C., Gramain, P. (1990) Kinetics of dissolution of calcium hydroxyapatite powder. Ill: pH and sample conditioning effects. Calcified Tissue International 46: 121129.
Williams, P. L., Warwick, R., Dyson, M., Bannister, L. H. (1989) Gray's Anatomy, 37th Edition. Churchill Livingstone, Edinburgh, pp 293299.


Long-term observation of subcutaneous tissue reaction to synthetic auditory ossicle (Apaceram®) in rats

  • Dong-Jun Li (a1), Katsuichiro Ohsaki (a1), Kunio Ii (a2), Qing Ye (a2), Yoko Nobuto (a1), Satoru Tenshin (a3) and Teruko Takano-Yamamoto (a3)...


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