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The incidence and detection of HPV in the upper aerodigestive tract using brush and biopsy techniques

Published online by Cambridge University Press:  29 June 2007

Richard H. Vowles ,
Steven Myint  and
Charles B. Croft
Richard H. Vowles*
Affiliation:
Department of Otolaryngology, Head and Neck Surgery, The Royal National Throat, Nose and Ear Hospital, Medical Sciences Building, University Road, Leicester, UK.
Steven Myint
Affiliation:
Department of Microbiology and Immunology, Medical Sciences Building, University Road, Leicester, UK.
Charles B. Croft
Affiliation:
Department of Otolaryngology, Head and Neck Surgery, The Royal National Throat, Nose and Ear Hospital, Medical Sciences Building, University Road, Leicester, UK.
*
Address for correspondence: Mr R. H. Vowles, Department of Otolaryngology, Head and Neck Surgery, The Royal National Throat, Nose and Ear Hospital, Gray's Inn Road, London WC1X 8DA.
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Abstract

The association between human papillomavirus (HPV) and squamous carcinoma is well established. In the polymerase chain reaction (PCR) we have an effective technique for detecting small quantities of viral DNA, but the assay requires tissue taken at endoscopy to reveal the presence or absence of HPV. Brushings have been used effectively elsewhere in the body to obtain cytological material. This study set out to ascertain if sufficient viral DNA could be obtained, using a laryngeal brushing and the PCR, to detect the presence or absence of HPV.

Six patients with squamous carcinoma of the larynx and seven controls who were having laryngoscopy for other reasons underwent laryngeal biopsy. In addition, in the patients with carcinoma, biopsies were taken at the tumour margins and brushings from both sites. The samples were tested for the presence of HPV types 6, 11, 16, 18 and 31 by means of the PCR. The distribution of HPV types was as expected in the biopsy specimens, but only one brushing detected any HPV type at all. We conclude that laryngeal brushing is an inadequate technique for assessing the presence of HPV in the larynx.

Keywords

Human papillomavirusPolymerase chain reactionCarcinoma, squamous cellBiopsyBrushing
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 3 , March 1997 , pp. 215 - 217
Copyright
Copyright © JLO (1984) Limited 1997

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References

Brandsma, J. L., Abramson, A. L. (1989) Association of papillomavirus with cancer of the head and neck. Archives of Otolaryngology, Head and Neck Surgery 115: 621–625.CrossRefGoogle Scholar
Bryan, R. L., Bevan, I. S., Crocker, J., Young, L. S. (1990) Detection of HPV 6 and 11 in tumours of the upper respiratory tract using the polymerase chain reaction. Clinical Otolaryngology 15: 177–180.Google Scholar
Covell, J. L., Frierson, H. F. (1992) Intraepithelial neoplasia mimicking microinvasive squamous-cell carcinoma in endocervical brushings. Diagnostic Cytopathology 8 (1): 18–22.CrossRefGoogle ScholarPubMed
Debeljak, A., Mermolja, M., Sorli, J., Zupancic, M., Zorman, M., Remskar, J. (1994) Bronchiolar lavage in the diagnosis of peripheral primary and secondary malignant lung tumours. Respiration 61: 226–230.CrossRefGoogle Scholar
Dekmezian, R., Batsakis, J., Goepfert, H. (1987) In situ hybridization of papillomavirus DNA in head and neck squamous cell carcinomas. Archives of Otolaryngology, Head and Neck Surgery 113: 819–821.CrossRefGoogle ScholarPubMed
Fuchs, P., Girardi, F., Pfister, H. (1988) Human papillomavirus DNA in normal, metaplastic, preneoplastic, and neoplastic epithelia of the cervix uteri. International Journal of Cancer 41: 41–45.Google Scholar
Gibson, P., Allen, C., Yang, J., Wong, B., Dolovich, J., Denburg, J., Hargreave, F. (1993) Intraepithelial mast cells in allergic and non-allergic asthma: assessment using bronchial brushings. American Review of Respiratory Diseases 148: 80–86.Google Scholar
Jalal, H., Sanders, C., Prime, S., Scully, C., Maitland, N. (1992) Detection of human papillomavirus type 16 DNA in oral squames from normal young adults. Journal of Oral Pathology and Medicine 21: 465–470.Google Scholar
Kashima, H., Kutcher, M., Keiss, T., Levin, S., De Villiers, E., Shah, K. (1990) Human papillomavirus in squamous cell carcinoma, leukoplakia, lichen planus, and clinically normal epithelium of the oral cavity. Annals of Otology, Rhinology and Laryngology 99: 55–61.CrossRefGoogle ScholarPubMed
Kashima, H., Mounts, P., Kuhajda, F., Loury, M. (1986) Demonstration of human papillomavirus capsid antigen in carcinoma in situ of the larynx. Annals of Otology, Rhinology and Laryngology 95: 603–607.CrossRefGoogle ScholarPubMed
Lehn, H., Villa, L., Marziona, F., Hilgarth, M., Hillemans, H., Sauer, G. (1988) Physical state and biological activity of human papillomavirus genomes in precancerous lesions of the female genital tract. Journal of General Virology 69: 187–196.CrossRefGoogle ScholarPubMed
Morgan, D., Abdullah, V., Quiney, R., Myint, S. (1991) Human papillomavirus and carcinoma of the laryngopharynx. Journal of Laryngology and Otology 105: 288–290.Google Scholar
Nunez, D., Astley, S., Lewis, F., Wells, M. (1994) Human papillomaviruses: a study of their prevalence in the normal larynx. Journal of Laryngology and Otology 108: 319–320.CrossRefGoogle Scholar
Popp, W., Merkle, M., Schreiber, B., Rauscher, H., Ritschka, L., Zwick, H. (1992) How much brushing is enough for the diagnosis of lung tumours? Cancer 70: 2278–2280.Google Scholar
Ryan, M., Baldauf, M. (1994) Comparison of flow cytometry for DNA content and brush cytology for detection of malignancy in pancreaticobiliary strictures. Gastrointestinal Endoscopy 40: 133–139.Google Scholar
Sherman, M., Anderson, C., Herman, L., Ludwig, M., Erozan, Y. (1994) Utility of gastric brushing in the diagnosis of malignant lymphoma. Acta Cytologica 38: 169–174.Google ScholarPubMed
Sonnex, C., Schofield, J., Kocjan, G., Kelly, G., Whatrup, C., Mindel, A., Northover, J. (1991) Anal human papillomavirus infection: a comparative study of cytology, colposcopy and DNA hybridization as methods of detection. Genitourinary Medicine 67: 21–25.Google ScholarPubMed