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Effect of intratympanic mitomycin C on the development of cholesteatoma and otitis media in rats

Published online by Cambridge University Press:  14 February 2013

A A Melo ,
S S Caldas Neto ,
F S Leão  and
A J C Campos
A A Melo*
Affiliation:
Department of Otolaryngology, Federal University of Pernambuco School of Medicine, Recife, Brazil
S S Caldas Neto
Affiliation:
Department of Otolaryngology, Federal University of Pernambuco School of Medicine, Recife, Brazil
F S Leão
Affiliation:
Department of Otolaryngology, Federal University of Pernambuco School of Medicine, Recife, Brazil
A J C Campos
Affiliation:
Department of Otolaryngology, Federal University of Pernambuco School of Medicine, Recife, Brazil
*
Address for correspondence: Dr Antonio Antunes Melo, Avenida José Augusto Moreira 685, Casa Caiada, CEP 53130-410, Olinda-PE, Brazil Fax: +55 8134922695 E-mail: antunes.orl@gmail.com
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Abstract

Objective:

To determine whether the administration of mitomycin C prevents propylene glycol exposure from inducing middle-ear cholesteatoma and otitis media, in a rat model.

Methods:

Twenty-four Wistar rats underwent intratympanic injections on days 1, 8 and 15, via the tympanic membrane pars tensa, in both the right and left ears. The right ear injection solution contained 50 per cent propylene glycol, gentamicin and saline (0.9 per cent), while the left ear solution contained 50 per cent propylene glycol, gentamicin and mitomycin C. Animals were sacrificed and examined.

Results:

There were statistically significant differences between the control and experimental groups for tympanic bulla mucosal thickness (p = 0.004) but not for tympanic membrane thickness (p = 0.371), otomicroscopic findings (p = 0.262), or the presence of exudate (p = 0.125), fibrosis (p = 1.000) or cholesteatoma (p = 0.687).

Conclusion:

Intratympanic mitomycin C was ineffective in preventing middle-ear cholesteatoma and otitis media in this rat model.

Keywords

Mitomycin CCholesteatomaOtitis MediaPropylene GlycolRatsWistar
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 4 , April 2013 , pp. 359 - 363
Copyright
Copyright © JLO (1984) Limited 2013

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Footnotes

Presented (and awarded a thesis prize) at the 39th Brazilian Congress of Otolaryngology and Head and Neck Surgery, 31 March to 4 April 2008, Belo Horizonte, Brazil

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