Hostname: page-component-76fb5796d-x4r87 Total loading time: 0 Render date: 2024-04-27T01:09:02.828Z Has data issue: false hasContentIssue false
  • English
  • Français

An evaluation of the use of scanning and transmission electronmicroscopy in a study of the gentamicin-damaged guinea-pig organ of Corti

Published online by Cambridge University Press:  29 June 2007

E. S. Harpur  and
J. B. Bridges
E. S. Harpur
Affiliation:
Pharmacology Laboratories, Department of Pharmacy, The University of Aston in Birmingham, Gosta Green, Birmingham B4 7ET.
J. B. Bridges
Affiliation:
Department of Physiology, The Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL.
Get access Rights & Permissions [Opens in a new window]

Abstract

Scanning electronmicroscopy and transmission electronmicroscopy were used to study the normal and gentamicin-damaged guinea-pig organ of Corti. Scanning electronmicroscopy was principally used to locate the lesion and quantify the cell loss but also permitted a detailed study to be made of the degenerative surface changes. However, since consistent surface changes are not seen until intracellular degeneration has progressed to a great extent, scanning electronmicroscopy is not a suitable technique for identifying early degenerative changes in the sensory cells of the organ of Corti. Consequently, before conclusions may be drawn about the absence of pathological changes in the neuro-epithelium it also should be examined using transmission electron-microscopy.

Type
Research Article
Information
The Journal of Laryngology & Otology , Volume 93 , Issue 1 , January 1979 , pp. 7 - 23
Copyright
Copyright © JLO (1984) Limited 1979

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bredberg, G., Ades, H. W., and Engström, H. (1972) Acta Oto-laryngologica, Supplement, 301, 3.CrossRefGoogle Scholar
Bredberg, G., Lindeman, H. H., Ades, H. W., West, R., and Engström, H. (1970) Science, 170, 861.CrossRefGoogle Scholar
Bridges, J. B., and Harpur, E. S. (1976) Journal of Physiology, 257, 42P.Google Scholar
Duvall, A. J., and Wersäll, J. (1964) Acta Oto-laryngologica, 57, 581.CrossRefGoogle Scholar
Engström, H., Ades, H. W., and Andersson, A. (1966) Structural pattern of the organ of Corti. Almqvist and Wiksell, Stockholm.Google Scholar
Engström, H., Ades, H. W., and Hawkins, J. E. Jr. (1964) Acta Oto-laryngologica, Supplement, 188, 92.CrossRefGoogle Scholar
Gonzalez, G., Miller, N., and Wasilewski, V. (1972) Annals of Otology, Rhinology and Laryngology, 81, 127.CrossRefGoogle Scholar
Hawkins, J. E. Jr. (1959) Annals of Otology, Rhinology and Laryngology, 68, 698.CrossRefGoogle Scholar
Hawkins, J. E. Jr. (1970) In: Biochemical mechanisms in hearing and deafness (ed. Paparella, M. M.), p. 323. Thomas, Springfield (Illinois).Google Scholar
Hawkins, J. E. Jr. (1973) Audiology, 12, 383.CrossRefGoogle Scholar
Hawkins, J. E. Jr., and Engstrom, H. (1964) Acta Oto-laryngologica, Supplement, 188, 100.CrossRefGoogle Scholar
Hawkins, J. E. Jr., Johnsson, L.-G., and Preston, R. E. (1972) Laryngoscope, 82, 1091.CrossRefGoogle Scholar
Hawkins, J. E. Jr., Rahway, N. J., and Lurie, M. H. (1952) Annals of Otology, Rhinology and Laryngology, 61, 789.CrossRefGoogle Scholar
Johnsson, L.-G., and Hawkins, J. E. Jr. (1972) Laryngoscope, 82, 1105.CrossRefGoogle Scholar
Kacker, S. K. (1970) Laryngoscope, 80, 391.CrossRefGoogle Scholar
Kohonen, A. (1965) Acta Oto-laryngologica, Supplement, 208, 9.Google Scholar
Kohonen, A., and Tarkkanen, J. (1969) Acta Oto-laryngologica, 68, 90.CrossRefGoogle Scholar
Lawrence, M. (1966) Laryngoscope, 76, 1318.CrossRefGoogle Scholar
Lim, D. J. (1976) Annals of Otology, Rhinology and Laryngology, 85, 740.CrossRefGoogle Scholar
Lim, D. J., and Lane, W. C. (1969a) Annals of Otology, Rhinology and Laryngology, 78, 827.CrossRefGoogle Scholar
Lim, D. J., and Lane, W. C. (1969b) Archives of Otolaryngology, 90, 283.CrossRefGoogle Scholar
Lundquist, P.-G., and Wersäll, J. (1966) Zeitschrift fiir Zellforschung und microskopische Anatomie, 72, 543.CrossRefGoogle Scholar
Lundquist, P.-G., and Wersäll, J. (1967) In: Gentamicin, First International Symposium, Paris.Google Scholar
Theopold, H.-M. (1977) Acta Oto-laryngologica, 84, 57.CrossRefGoogle Scholar
Wersäll, J. (1973) In: Basic Mechanisms in Hearing (ed. Møller, A. R.), p. 235. Academic Press, New York and London.CrossRefGoogle Scholar
Wersäll, J., Björkroth, B., Flock, Å., and Lundquist, P.-G. (1973) In: Advances in Oto-Rhino-Laryngology, 20, 14. Karger, Basel.Google Scholar
Wright, I. (1969) Journal of Pathology, 98, 129.CrossRefGoogle Scholar
Ylikoski, J. (1974) Acta Oto-laryngologica, Supplement, 326, 5.CrossRefGoogle Scholar