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Wuchereria bancrofti recombinant antigen-derived poly- and monoclonal antibodies for the detection of circulating antigen(s) in the sera of lymphatic filarial patients

Published online by Cambridge University Press:  05 June 2009

J.G. Theodore  and
P. Kaliraj
J.G. Theodore
Affiliation:
Center for Biotechnology, Anna University, Madras 600 025, India
P. Kaliraj*
Affiliation:
Center for Biotechnology, Anna University, Madras 600 025, India
*
* Corresponding author
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Abstract

A sandwich antibody ELISA was employed for the detection of circulating filarial antigen in patients with bancroftian filariasis. Wuchereria bancrofti recombinant antigen-derived polyclonal and monoclonal antibodies were successfully used as the revealing antibodies and their efficiency was compared. All the microfilariae (mf) positive (by finger prick and examination of 20 μl of blood under the microscope) individuals tested showed the presence of circulating antigen(s). Among the antigen positive endemic normals (mf negative by the finger prick method), 43% showed microfilariae by a sensitive parasitological method viz. membrane filtration of the night blood samples. A significant correlation was observed between the parasite antigen levels and the blood microfilaria counts among the mf carriers. This information on the parasite antigen levels could be an ideal monitor to indicate the degree of active infection and in the follow up of chemotherapy.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 1 , March 1996 , pp. 69 - 74
Copyright
Copyright © Cambridge University Press 1996

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References

Au, A.C.S., Denham, D.A., Steward, M.W., Draper, C.C., Ismail, M.M., Rao, C.K. & Mak, J.W. (1981) Detection of circulating antigens and immune complexes in feline and human lymphatic filariasis. South-east Asian Journal of Tropical Medicine and Public Health 12, 492–498.Google ScholarPubMed
Chanteau, S., Moulia-Pelat, J.P., Glaziou, P., Nguyen, N.L., Luquiand, P., Plichart, C, Martin, P.M.V. & Cartel, J.L. (1994) Og4C3 circulating antigen: A marker of infection and adult worm burden in Wuchereria bancrofti filariasis. Journal of Infectious Diseases 170, 247–250.CrossRefGoogle ScholarPubMed
Dasgupta, A., Bala, S. & Dutta, S.N. (1984) Lymphatic filariasis in man demonstration of circulating antigens in Wuchereria bancrofti infection. Parasite Immunology 6, 341–348.CrossRefGoogle ScholarPubMed
Dissanayake, S., Forsyth, K.P., Ismail, M.M. & Mitchell, G.F. (1984) Detection of circulating antigen in bancroftian filariasis by using a monoclonal antibody. American Journal of Tropical Medicine and Hygiene 33, 1130–40.CrossRefGoogle ScholarPubMed
Forsyth, K.P., Spark, R., Kazura, J., Brown, G.V., Peters, P., Heywood, P., Dissanayake, S. & Mitchell, G.F. (1985) A monoclonal antibody based immunoradioactive assay for detection of circulating antigen in bancroftian filariasis. Journal of Immunology 134, 1172–1177.CrossRefGoogle Scholar
Franks, M.B. (1946) Specific soluble antigen in the blood of filarial patients. Journal of Parasitology 32, 400–406.CrossRefGoogle ScholarPubMed
Hamilton, R.G., Hussain, R. & Ottessen, E.A. (1984) Immunoradiometric assay for detection of filarial antigens in human serum, Journal of Immunology 133, 2237–2242.CrossRefGoogle ScholarPubMed
Harinath, B.C. (1984) Immunodiagnosis of bancroftian filariasis - problems and progress. Journal ofBioscience 6, 691.CrossRefGoogle Scholar
Harlow, E. & Lane, D. (1988) Antibodies: a laboratory manual. Cold Spring Harbour.Google Scholar
Kaliraj, P., Ghirnikar, S.N. & Harinath, B.C. (1979) Indirect fluorescent antibody technique using sonicated Wuchereria bancrofti microfilaria for immunodiagnosis of bancroftian filariasis. Indian Journal of Experimental Biology 17, 332–335.Google ScholarPubMed
Lal, R.B., Paranjape, R.S., Briles, D.E., Nutman, T.B. & Oftesen, E.A. (1987) Circulating parasite antigen(s) in lymphatic filariasis: use of monoclonal antibodies to phosphorylcholine for immunodiagnosis. Journal of Immunology 138, 3454–3460.CrossRefGoogle ScholarPubMed
Michael, E. & Bundy, D.A.P. (1994) Informal WHO consultation.Google Scholar
More, S.J. & Copeman, D.B. (1990) A highly specific and sensitive monoclonal and antibody based ELISA for the detection of circulating antigens in bancroftian filariasis, Tropical Medicine and Parasitology 41, 403–406.Google ScholarPubMed
Ottesen, E.A., Weller, P.F., Lunde, M.N. & Hussain, R. (1982) Endemic filariasis on a Pacific island 11. Immunologic aspects: Immunoglobulin complement and specific antifilarial IgG, IgM and IgE antibodies. American Journal of Tropical Medicine and Hygiene 31, 953–961.CrossRefGoogle Scholar
Paranjape, R.S., Hussian, R., Nutman, T.B., Hamilton, R. & Ottesen, E.A. (1986) Identification of circulating parasite antigen in patients with bancroftian filariasis. Clinical and Experimental Immunology 63, 508.Google ScholarPubMed
Quassi, A., Kouemeni, L.E., Haque, A., Ridel, P.R., Andre, P.S. & Capron, A. (1981) Detection of circulating antigens in onchocerciasis. American Journal of Tropical Medicine and Hygiene 30, 1211–1218.Google Scholar
Raghavan, N., McReynolds, L.A., Maina, C.V., Feinstone, S.M., Jayaraman, K., Ottesen, E.A. & Nutman, T.B. (1991) A recombinant clone of W. bancrofti with DNA specificity for human lymphatic filarial parasites. Molecular and Biochemical Parasitology 47, 63–72.CrossRefGoogle ScholarPubMed
Theodore, G.J. & Kaliraj, P. (1990) Isolation, purification and characterization of surface antigens of the bovine filarial parasite Setaria digitata for the immunodiagnosis of bancroftian filariasis. Journal of Helminthology 64, 105–114.CrossRefGoogle ScholarPubMed
Theodore, G.J., Kaliraj, P., Jayachandran, S. & Jayaraman, K. (1993) Cloning, over expression and evaluation of a recombinant fusion protein of Wuchereria bancrofti towards its application as a diagnostic agent for bancroftian filariasis. Parasitology 106, 413–420.CrossRefGoogle ScholarPubMed
Weil, G.J., Kumar, H., Santhanam, S., Sethumadhavan, K.V. & Jain, D.C. (1986) Detection of circulating parasite antigen in bancroftian filariasis by counter immunoelectrophoresis. American Journal of Tropical Medicine and Hygiene 35, 565–570.CrossRefGoogle Scholar
Weil, G.J. & Liftis, F. (1987) Identification and partial characterization of a parasite antigen in sera from humans infected with W. bancrofti. Journal of Immunology 138,3035–3041.CrossRefGoogle Scholar
Weil, G.J., Jain, D.C., Santhanam, S., Malhotra, A., Kumar, H., Sethumadhavan, K.V., Lifitis, F. & Ghosh, T.K. (1987) A monoclonal antibody based enzyme immunoassay for detecting parasite antigenemia in bancroftian filariasis. Journal of Infectious Diseases 156, 350–355.Google Scholar
Zheng, H.J., Tao, Z.H., Reddy, M.V., Harinath, B.C. & Piessens, W.G. (1987) Parasite antigens in sera and urine of patients with bancroftian and brugian filariasis detected by sandwich ELISA with monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 36, 554–560.Google ScholarPubMed