Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-06-30T10:47:57.939Z Has data issue: false hasContentIssue false
  • English
  • Français

The utility of human filarial serum in the detection of circulating antigen

Published online by Cambridge University Press:  18 November 2009

P. Kaliraj ,
Indira Kharat ,
S. N. Ghirnikar  and
B. C. Harinath
P. Kaliraj
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India–442 102
Indira Kharat
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India–442 102
S. N. Ghirnikar
Affiliation:
Research cum Training Centre, Wardha
B. C. Harinath*
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India–442 102
*
†Address for correspondence: Prof. B. C. Marinath, Department of Biochemistry, M. G. Inst. of Med. Sciences, Sevagram, Wardha (dt), Maharashtra, India-442 102.
Get access Rights & Permissions [Opens in a new window]

Abstract

The utility of human filarial serum immunoglobulin (FSI) in detecting circulating antigen in filarial sera was studied by counter Immunoelectrophoresis (CIEP) and the indirect haemagglutination test (IHAT). CIEP was found to be better than IHAT. 23 out of 30 sera from persons with microfilaraemia and one of 30 clinical cases of filariasis, but none of the normal sera or sera from those with helminths other than filariae, showed the presence of circulating filarial antigen in CIEP. FSI was fractionated by DEAE- Sephadex A-50 column chromatography and the antibody active in CIEP was found to be IgG in nature.

Type
Research Article
Information
Journal of Helminthology , Volume 55 , Issue 2 , June 1981 , pp. 133 - 140
Copyright
Copyright © Cambridge University Press 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Berggren, W. L. & Weller, T. H. (1967) Immunoelectrophoretic demonstration of specific circulating antigen in animals infected with Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene, 16, 606–612.Google Scholar
Carlier, Y., Bout, D., Bina,, J. C., Camus, D., Figueirede, J. F. M. & Capron, A. (1975) Immunological studies in human schistosomiasis. I. Parasitic antigen in urine.American Journal of Tropical Medicine and Hygiene, 24, 949–954.CrossRefGoogle ScholarPubMed
Dasgupta, A. & Bala, Shukal (1978) Litomosoides carini—isoluble antigen in circulation and immunosuppression in vitro. Indian Journal of Medical Research, 67, 30–33.Google ScholarPubMed
Deelder, A. M. & Eveleigh, P. C. (1978) An indirect haemagglutination reaction for the demonstration of Schistosoma mansoni circulating anodic antigen. Transactions of the Royal Society of Tropical Medicine and Hygiene, 72, 178–180.Google Scholar
Desowitz, R. S. & Una, S. R. (1976) The detection of antibodies in human and animal filariasis by Counter current immunoelectrophoresis with Dirofilaria immitis antigen. Journal ofHelminthology, 50, 53–57.Google Scholar
Ferreira, A. W., Caldini, A. L. U., Hoshino-Shimizu, S. & Camargo, M. E. (1979) Immuno-enzymatic assay for the detection of Schistosoma mansoni antigens in serum of mice harbouring bisexual or unisexual light worm infections. Journal of Helminthology, 53, 189–194.Google Scholar
Franks, M. B. (1946). Specific soluble antigen in the blood of filarial patients. Journal of Parasitology, 32, 400–406.CrossRefGoogle ScholarPubMed
Gold, R., Rosein, F. S. & Weller, T. H. (1969) A specific circulating antigen in hamsters infected with Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene, 18, 545–552.CrossRefGoogle ScholarPubMed
Harinath, B. C. & KallRaj, P. (1979). Immunodiagnosis of bancroftian filariasis with microfilarial antigens: Problems and Progress. Paper presented at Tropical Immunology Seminar, Nov. 27–Dec, 4, 1979., Sydney (Australia).Google Scholar
Herbert, W. J. (1967) Passive haemagglutination. In: Handbook of Experimental Immunology (editor, Weir, D. M.), pp. 720–744. Blackwell Scientific Publications, Oxford.Google Scholar
Kaliraj, P., Ghirnikar, S. N. & Harinath, B. C. (1979a) Indirect fluorescent antibody technique using sonicated Wuchereria bancrofti microfilariae for immunodiagnosis of bancroftian filariasis. Indian Journal of Experimental Biology, 17, 332–335.Google Scholar
Kaliraj, P., Ghirnikar, S. N. & Harinath, B. C. (1979b) Detection of circulating filarial antigen in bancroftian filariasis. Indian Journal of Experimental Biology, 17, 1148–1149.Google Scholar
Kaliraj, P., Ghirnikar, S. N. & Harinath, B. C. (1981) Enzyme linked immunosorbent assay for bancroftian filariasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 75, 119–120.Google Scholar
Nash, T. E., Nasir, U. D. & Jeanloz, R. W. (1977) Further purification and characterization of a circulating antigen in Schistosomiasis. Journal of Immunology, 119, 1627–1643.Google Scholar
Ouchterlony, O. (1949) Antigen-antibody reaction in gels. Acta Pathologica et Microbiologica Scandinavica, 26, 507–515.Google Scholar
Santoro, F., Carlier, Y., Borojevic, R., Bout, D., Tachon, P. & Capron, A. (1977) Parasite ‘M’ antigen in milk from mothers infected with Schistosoma mansoni (Preliminary report). Annals of Tropical Medicine and Parasitology, 71, 121–123.Google Scholar
Tanabe, N. (1959) Immunoserological studies in filariasis. III. Cutaneous reaction and precipitin test with Wuchereria antigens and evidence of antigenicity of urine from filariasis patients. Endemic Disease Bulletin of Nagasaki University, 1, 38–50.Google Scholar