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Toxocara infection in pigs. The use of indirect fluorescent antibody tests and an in vitro larval precipitate test for detecting specific antibodies

Published online by Cambridge University Press:  05 June 2009

P. Stevenson  and
D. E. Jacobs
P. Stevenson
Affiliation:
Department of Pathology, Royal Veterinary College, University of London, North Mymms, Hatfield, Hertfordshire
D. E. Jacobs
Affiliation:
Department of Pathology, Royal Veterinary College, University of London, North Mymms, Hatfield, Hertfordshire
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Abstract

An in vitro larval precipitate test using second-stage Toxocara canis larvae and an indirect fluorescent antibody (IFA) test employing cuticles of T. canis larvae as antigen were evaluated using antisera produced in pigs experimentally infected with T. canis, T. cati, Ascaris suum, Toxascaris leonina and Parascaris equorum. The former test was both specific and sensitive and is suggested as a reliable and simple method of detecting Toxocara antibodies in pigs. The latter test was considered unsuitable because of cross-reactions that occurred when sera from pigs infected with other ascarids were tested. An IFA test for Ascaris antibodies, employing cuticles of A. suum larvae as antigen, is described. The degree of specificity of this test suggests that it may be of value in the detection of antibodies to Ascaris in pigs under natural conditions.

Type
Research Article
Information
Journal of Helminthology , Volume 51 , Issue 2 , June 1977 , pp. 149 - 154
Copyright
Copyright © Cambridge University Press 1977

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References

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