Skip to main content Accessibility help

Toxocara infection in pigs. The use of indirect fluorescent antibody tests and an in vitro larval precipitate test for detecting specific antibodies

  • P. Stevenson (a1) and D. E. Jacobs (a1)


An in vitro larval precipitate test using second-stage Toxocara canis larvae and an indirect fluorescent antibody (IFA) test employing cuticles of T. canis larvae as antigen were evaluated using antisera produced in pigs experimentally infected with T. canis, T. cati, Ascaris suum, Toxascaris leonina and Parascaris equorum. The former test was both specific and sensitive and is suggested as a reliable and simple method of detecting Toxocara antibodies in pigs. The latter test was considered unsuitable because of cross-reactions that occurred when sera from pigs infected with other ascarids were tested. An IFA test for Ascaris antibodies, employing cuticles of A. suum larvae as antigen, is described. The degree of specificity of this test suggests that it may be of value in the detection of antibodies to Ascaris in pigs under natural conditions.



Hide All
Annen, J. M., Eckert, J. and Hess, U. (1975) Eine einfache Methode zur Gewinnung von Toxocara canis—Antigen für die indirekte Immunofluoreszenz-Technik. Acta Tropica, 32, 3747.
Bisseru, B. and Woodruff, A. W. (1968) The detection of circulating antibody in human toxocara infections using the indirect fluorescent antibody test. Journal of Clinical Pathology, 21, 449455.
Diconza, J. J. (1972) Toxocara canis: Some characteristics of larval precipitating antibodies in rat serum. International Journal for Parasitology, 2, 471479.
Done, J. T., Richardson, M. D., and Gibson, T. E. (1960) Experimental visceral larva migrans in the pig. Research in Veterinary Science, 1, 133151.
Hayden, D. W. and Van Kruiningen, H. J. (1975) Experimentally induced canine toxocariasis: Laboratory examinations and pathologic changes, with emphasis on the gastrointestinal tract. American Journal of Veterinary Research, 36, 16051614.
Hogarth-Scott, R. S. (1966) Visceral larva migrans—an immunofluorescent examination of rabbit and human sera for antibodies to the ES antigens of the second stage larvae of Toxocara canis, Toxocara cati and Toxascaris leonina (Nematoda). Immunology, 10, 217223.
Jaskoski, B. J. and Colucci, A. V. (1964) In vitro hatching of Ascaris suum eggs. Transactions of the American Microscopical Society, 83, 294300.
Jung, R. C. (1973) Problems of diagnosis and management of visceral larva migrans and other cryptic parasitoses. Yonsei Reports on Tropical Medicine, 4, 96101.
Kagan, I. G. (1968) Serologic diagnosis of visceral larva migrans. Clinical Pediatrics, 7, 508509.
Lamina, J. (1970) Immunobiologischer Nachwesis einer “Larva migrans viseceralis-Infektion”. II. Mitteilung: Der Mirkropräzipitationstest an der lebenden Larven. Zentralbatt für Bakteriologie und Parasitenkunde, I. Abt. Orig., 215, 386397.
Mitchell, J. R. (1964) Detection of Toxocara canis antibodies with the fluorescent antibody technique. Proceedings of the Society for experimental Biology and Medicine, 117, 267270.
Olson, L. J. (1960) Serology of visceral larva migrans: In vitro larval precipitate test. Texas Reports on Biology and Medicine, 18, 473479.
Roneus, O. (1966) Studies on the aetiology and pathogenesis of white spots in the liver of pigs. Acta veterinaria Scandinavica, 7, Suppl.16.
Viens, P., Strykowski, H., Richards, R. and Sonera, S. (1975) A modified immunofluorescent antibody technique for the serodiagnosis of human toxocaral larva migrans. Canadian Journal of Public Health, 66, 237240.
Woodruff, A. W.. (1970) Toxocariasis. British Medical Journal, 3, 663669.
Woodruff, A. W. (1975) Clinical problems of preventive medicine. Toxocara canis and other nematodes transmitted from dogs to man. British Veterinary Journal, 131, 627632.

Toxocara infection in pigs. The use of indirect fluorescent antibody tests and an in vitro larval precipitate test for detecting specific antibodies

  • P. Stevenson (a1) and D. E. Jacobs (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.