Hostname: page-component-848d4c4894-pftt2 Total loading time: 0 Render date: 2024-05-17T00:33:59.527Z Has data issue: false hasContentIssue false
  • English
  • Français

Stage-specific expression of a developmentally regulated gene in Dirofilaria immitis

Published online by Cambridge University Press:  05 June 2009

S. Sun ,
T. Matsuura  and
K. Sugane
S. Sun
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
T. Matsuura
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
K. Sugane*
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
*
*Author to whom correspondence should be addressed.
Get access Rights & Permissions [Opens in a new window]

Abstract

A previously reported cDNA clone encoding 34 kDa antigenic polypeptide of Dirofilaria immitis (λ cD34) was studied to elucidate the mechanism of stage-specific gene expression. The 34 kDa polypeptide was a larva-specific antigen and the mRNA was detectable in microfilariae but not in adult worms and eggs. The λ cD34 gene was not sex linked and was contained in the genome of D. immitis at each stage. The stage-specific expression of the developmentally regulated gene in D. immitis may be controlled primarily at the mRNA level.

Keywords

Dirofilaria immitiscDNA clonelarva-specific antigendevelopmentally regulated gene
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 1 , March 1992 , pp. 62 - 67
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abraham, D., Mok, M., Mika-Grieve, M. & Grieve, R. B. (1987) In vitro culture of Dirofilaria immitis third- and fourth-stage larvae under defined conditions. Journal of Parasitology, 73, 377–383.CrossRefGoogle ScholarPubMed
Abraham, D., Grieve, R. B. & Mika-Grieve, M. (1988) Dirofilaria immitis: Surface properties of third- and fourth-stage larvae. Experimental Parasitology. 65, 157–167.CrossRefGoogle ScholarPubMed
Aviv, H. & Leder, P. (1972) Purification of biologically active globin messenger RNA by chromatography of oligothymidylic acid-cellulose. Proceedings of the National Academy of Sciences (USA), 69, 1408–1412.CrossRefGoogle ScholarPubMed
Beard, C. A., Wrightsman, R. A. & Manning, J. E. (1988) Stage and strain specific expression of the tandemly repeated 90 kDa surface antigen gene family in Trypanosoma cruzi. Molecular and Biochemical Parasitology. 28, 227–234.CrossRefGoogle ScholarPubMed
Bobek, L., Rekosh, D. M., Keulen, H. V. & Loverde, P. T. (1986) Characterization of a female-specific cDNA derived from a developmentally regulated mRNA in the human blood fluke Schistosoma mansoni. Proceedings of tile National Academy of Sciences (USA). 83, 5544–5548.CrossRefGoogle ScholarPubMed
Davis, A. H., Blanton, R., Rottman, F., Maurer, R. & Mahmoud, A. (1986) Isolation of cDNA clones for differentially expressed genes of the human parasite Schistosoma mansoni. Proceedings of the National Academy of Sciences (USA). 83, 5534–5538.CrossRefGoogle ScholarPubMed
Feinberg, A. P. & Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry, 132, 6–13.CrossRefGoogle ScholarPubMed
Ibrahim, M. S., Tamashiro, W. K., Moraga, D. A. & Scott, A. L. (1989) Antigen shedding from the surface of the infective stage larvae of Dirofilaria immitis. Parasitology, 99, 89–97.CrossRefGoogle ScholarPubMed
Jeska, E. L. & Caruso, J. P. (1986) Collection of fertile Ascaris suum eggs. Journal of Parasitology, 72, 964–965.CrossRefGoogle ScholarPubMed
Kazura, J. W., Cicirello, H. & Forsyth, K. (1986) Differential recognition of a protective filarial antigen by antibodies from humans with bancroftian filariasis. Journal of Clinical Investigation, 77, 1985–1992.CrossRefGoogle ScholarPubMed
Kidane, G. Z., Samaras, N. & Spithill, T. W. (1989) Cloning of developmentally regulated genes from Leishmania major and expression following heat induction. Journal of Biological Chemistry, 264, 4244–4250.CrossRefGoogle ScholarPubMed
Maina, C. V., Grandea, A. G. III, Tuyen, L. T. K., Asikin, N., Williams, S. A. & McReynolds, L. A. (1987) Dirofilaria immitis: Genomic complexity and characterization of a structural gene. Molecular Paradigms for Eradicating Heleminthic Parasites, pp. 193–204.Google Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.Google Scholar
Ottesen, E., Weller, P. & Heck, L. (1977) Specific cellular immune unresponsiveness in human filariasis. Immunology, 33, 413–421.Google ScholarPubMed
Philipp, M. & Davis, T. B. (1986) Biochemical and immunologic characterization of a major surface antigen of Dirofilaria immitis infective larvae. Journal of immunology, 136, 2621–2627.CrossRefGoogle Scholar
Simpson, A. J. G., Chaudri, M., Knight, M., Kelly, C., Rumjanek, F., Martin, S. & Smithers, R. (1987) Characterisation of the structure and expression of the gene encoding a major female specific polypeptide of Schistosoma mansoni. Molecular and Biochemical Parasitology, 22, 169–176.CrossRefGoogle Scholar
Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology, 98, 503–517.CrossRefGoogle ScholarPubMed
Sun, S-H., Matsura, T. & Sugane, K. (1991) Molecular cloning of the cDNA encoding an immunodominant antigen of Dirofilaria immitis. Journal of Helminthology. 65, 149–158.CrossRefGoogle ScholarPubMed
Thomas, P. S. (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of National Academy of Sciences (USA), 77, 5201–5206.CrossRefGoogle ScholarPubMed
Towbin, H., Staehelin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gets to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Sciences (USA). 76, 4350–4354.CrossRefGoogle Scholar
Weinberger, C., Hollengerg, S. M., Ong, E. S., Harmon, J. M., Brown, S. T., Cidlowski, J., Thompson, E. B., Rosenfeld, M. G. & Evans, R. M. (1985) Identification of human glucocorticoid receptor complementary DNA clones by epitope selection. Science, 228, 740–742.CrossRefGoogle ScholarPubMed