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Specific IgG and immune complex responses to parthenogenetic females and eggs of nematode Strongyloides venezuelensis for the diagnosis of immunosuppression in infected rats

  • A.L.R. Gonçalves (a1), K.C.L. de Araújo (a1), E.F.G. Carvalho (a1), M.T. Ueta (a2) and J.M. Costa-Cruz (a1)...

Abstract

In the present study, antigens from parthenogenetic females and eggs of Strongyloides venezuelensis, or anti-parthenogenetic-female and anti-egg antigens were used to detect specific IgG and immune complex responses, respectively. Serum samples from experimentally infected immunocompetent and immunosuppressed rats were analysed on days 5, 8, 13 and 21 post-infection (dpi). An enzyme-linked immunosorbent assay (ELISA) was performed using alkaline parasite extract for specific IgG detection, and anti-parthenogenetic-female or anti-egg antigens for immune complex detection. The data were analysed using analysis of variance (ANOVA), followed by a Bonferroni test. When parthenogenetic female or egg extracts were used as antigens, specific IgGs were not detected in either immunocompetent or immunosuppressed rats. When anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used, immune complexes were detected for the duration of the infection in immunosuppressed animals and were only detected between 5 and 13 dpi in immunocompetent animals. The duration of infection was not significantly different between the immunocompetent and immunosuppressed groups when anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used. Parthenogenetic female extracts yielded significant differences between antibody and immune complex responses in immunocompetent rats from 5 to 13 dpi, but only on day 5 dpi in immunosuppressed rats. Exposure to S. venezuelensis egg extract yielded significant differences in both antibody and immune complex detection between immunocompetent and immunosuppressed rats for the duration of the infection. In conclusion, ELISA using alternative antigens may be a successful strategy for identifying immune complexes in serum samples and diagnosing active strongyloidiasis, particularly under conditions of immunosuppression.

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*E-mail: costacruz@ufu.br

References

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Gonçalves, A.L.R., Ribeiro, T.S., Silva, C.V., Ueta, M.T. & Costa-Cruz, J.M. (2012a) A novel approach based on antigen, antibody and immune complex detection in bronchoalveolar lavage fluid samples from rats experimentally infected with Strongyloides venezuelensis . Acta Tropica 124, 166169.
Gonçalves, A.L.R., Rocha, C.A., Gonzaga, H.T., Gonçalves-Pires, M.R.F., Ueta, M.T. & Costa-Cruz, J.M. (2012b) Specific IgG and IgA to larvae, parthenogenetic females and eggs of Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis. Diagnostic Microbiology and Infectious Disease 72, 7984.
Gonçalves, A.L.R., Silva, C.V., Ueta, M.T. & Costa-Cruz, J.M. (2012c) Antigen, antibody and immune complex detection in serum samples from rats experimentally infected with Strongyloides venezuelensis . Experimental Parasitology 130, 205208.
Gonzaga, H.T., Ribeiro, V. da S., Cunha-Júnior, J.P., Ueta, M.T. & Costa-Cruz, J.M. (2011) Usefulness of concanavalin-A non-binding fraction of Strongyloides venezuelensis larvae to detect IgG and IgA in human strongyloidiasis. Diagnostic Microbiology and Infectious Disease 70, 7884.
Gordon, H.M. & Whitlock, H.V.A. (1939) New technique for counting nematode eggs in sheep faeces. Journal of the Council for Scientific and Industrial Research 12, 5052.
Grove, D.I. (1996) Human strongyloidiasis. Advances in Parasitology 38, 251309.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265275.
Machado, E.R., Ueta, M.T., Gonçalves-Pires, M.R.F., Oliveira, J.B.A., Faccioli, L.H. & Costa-Cruz, J.M. (2003) Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay. Memórias do InstitutoOswaldo Cruz 98, 849851.
Paula, F.M., Sitta, R.B., Malta, F.M., Gottardi, M., Corral, M.A., Gryschek, R.C.B. & Chieffi, P.P. (2013) Parasitological and molecular diagnosis in experimental Strongyloides venezuelensis infection. Revista do Instituto de Medicina Tropical de São Paulo 55, 141143.
Rodrigues, R.M., Cardoso, C.R., Gonçalves, A.L.R., Silva, N.M., Massa, V., Alves, R., Ueta, M.T., Silva, J.S. & Costa-Cruz, J.M. (2013) Increased susceptibility to Strongyloides venezuelensis infection is related to the parasite load and absence of major histocompatibility complex (MHC) class II molecules. Experimental Parasitology 135, 580586.
Romand, S., Thulliez, P. & Dubey, J.P. (1998) Direct agglutination test for serologic diagnosis of Neospora caninum infection. Parasitology Research 84, 5053.
Rugai, E., Mattos, T. & Brisola, A.P. (1954) Nova técnica para isolar larvas de nematóides das fezes: Modificação do método de Baermann. Revista do Instituto Adolfo Lutz 14, 58.
Sato, Y. & Toma, H. (1990) Strongyloides venezuelensis infections in mice. International Journal of Parasitology 20, 5762.
Wilson, M.B. & Nakane, P.K. (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. p. 215 in Knapp, W., Holuban, K. & Wick, G. (Eds) Immunofluorescence and related techniques. Amsterdam, North-Holland Biomedical.
Yasuda, K., Matsumoto, M. & Nakanishi, K. (2014) Importance of both innate immunity and acquired immunity for rapid expulsion of S. venezuelensis . Frontiers in Immunology 5, 15.

Specific IgG and immune complex responses to parthenogenetic females and eggs of nematode Strongyloides venezuelensis for the diagnosis of immunosuppression in infected rats

  • A.L.R. Gonçalves (a1), K.C.L. de Araújo (a1), E.F.G. Carvalho (a1), M.T. Ueta (a2) and J.M. Costa-Cruz (a1)...

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