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Sodium dodecyl sulphate as a rapid clearing agent for studying the hard parts of monogeneans and nematodes

Published online by Cambridge University Press:  12 April 2024

W.L. Wong
Affiliation:
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
W.B. Tan
Affiliation:
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
L.H.S. Lim*
Affiliation:
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
*
*Author for correspondence Fax: +603 7967 4178 E-mail: susan@um.edu.my

Abstract

Hard structures of helminths have often been used for taxonomic identification but are usually not clearly defined when treated with conventional methods such as ammonium picrate-glycerin for monogeneans and glycerin for nematodes. The present study reports a rapid and simple technique to better resolve the hard parts of selected monogeneans and nematodes using 5–10% alkaline sodium dodecyl sulphate (SDS). In comparison with established methods, SDS-treated worms become more transparent. In monogeneans treated with SDS, clear details of the hooks, hook filaments, anchors, bars and the sclerotized copulatory organs could be observed. In SDS-treated nematodes, spicules and ornamentations of the buccal capsules could be clearly seen.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2006

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References

Kritsky, D.C., Leiby, P.D. & Kayton, R.J. (1978) A rapid stain technique for haptoral bars of Gyrodactylus species (Monogenea). Journal of Parasitology 69, 862865.Google Scholar
Lim, L.H.S. & Furtado, J.I. (1986) Sixteen new species of Dactylogyrus from the genus Puntius Hamilton (Cyprinidae). Folia Parasitologica 33, 2134.Google Scholar
Malmberg, G. (1956) Om förekomsten av Gyrodactylus på svenska fiskar. Skrifter utigivna av Södra Sveriges FiskeriföreningÅrsskrift 1976.Google Scholar
Richards, G.R. & Chubb, J.C. (1995) Trichrome staining of Gyrodactylus sclerites and soft tissues following fixation in ammonium picrate-glycerin, including an improved rendition of the haptoral bars of, G. turnbulli . Journal of Helminthology 69, 149154.CrossRefGoogle ScholarPubMed
Zdárska, Z. (1976) Selective staining of opisthaptor sclerites of some monogeneans. Folia Parasitologica 23, 182.Google ScholarPubMed