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Purification and properties of haemoglobin from Gastrothylax crumenifer (Trematode:Paramphistomatidae)

  • Jawed Siddiqui (a1) and Ather H. Siddiqi (a1)

Abstract

Gastrothylax crumenifer haemoglobin was isolated, purified, chromatographed, and its molecular weight determined on a calibrated Sephadex G–100 column as well as by sodium dodecyl sulphate by the former and 16 500 by the latter method. The oxygen affinity of the pigment (P50O2) was found to be 6·1 mm Hg at pH 7·4.

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Purification and properties of haemoglobin from Gastrothylax crumenifer (Trematode:Paramphistomatidae)

  • Jawed Siddiqui (a1) and Ather H. Siddiqi (a1)

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