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Purification and characterization of a glycoprotein from the surface of Ascaridia galli*

Published online by Cambridge University Press:  05 June 2009

Khalid Masood
Affiliation:
Central Drug Research Institute, Lucknow 226001, India
K. P. Sircar
Affiliation:
Division of Biochemistry and HPLC UnitLucknow 226001, India
V. M. L. Srivastava
Affiliation:
Central Drug Research Institute, Lucknow 226001, India

Abstract

Employing papain as the enzyme and agarose bound Ricinus communis agglutinin as the affinity gel, a glycoprotein has been isolated and purified from the surface of Ascaridia galli. The glycoprotein shows an apparent molecular weight of 68 kilo daltons and contains fucose, galactose, rhamnose and glucosamine as sugar moieties. Only 2% of its entire molecule has been found to possess α-helical configuration.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1987

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