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Protection of sheep against Schistosoma bovis using cryopreserved radiation-attenuated schistosomula

Published online by Cambridge University Press:  18 November 2009

E. R. James ,
A. R. Dobinson ,
B. J. Andrews ,
Q. D. Bickle ,
M. G. Taylor  and
P. J. Ham
E. R. James
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St. Albans, Herts, ALA 0XQ
A. R. Dobinson
Affiliation:
Department of Morbid Anatomy, University College Hospital, London WC1, UK
B. J. Andrews
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St. Albans, Herts, ALA 0XQ
Q. D. Bickle
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St. Albans, Herts, ALA 0XQ
M. G. Taylor
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St. Albans, Herts, ALA 0XQ
P. J. Ham
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St. Albans, Herts, ALA 0XQ
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Abstract

Three sheep were vaccinated with two doses of 3 Krad-irradiated cryopreserved Schistosoma bovis schistosomula containing 20,000 and 17,000 organisms respectively, injected intramusculary 23 days apart after storage in liquid nitrogen for between 9 and 46 days. A challenge of 5360 S. bovis cercariae was administered percutaneously approximately four weeks after the last vaccine dose to these animals and to three controls. Post-challenge the vaccinated animals gained significantly more weight (27%v.9%), produceed fewer eggs in their facces, showed a smaller reduction in PCV values (–18%v, –27%) and were over-all in better condition than control animals . At perfusion 49·1% fewer adult worms were found in the vaccinated sheep than in controls. The tissue egg burdens were similar in both groups. Histopathologically both groups were similar except that fewer and smaller egg lesions were observed in the livers of vaccinated animals.

Type
Research Papers
Information
Journal of Helminthology , Volume 59 , Issue 1 , March 1985 , pp. 51 - 55
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

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