Skip to main content Accessibility help
×
Home

Proliferation of pronephric lymphocytes of carp, Cyprinus carpio induced by extracts of Bothriocephalus acheilognathi

  • P. Nie (a1), D. Hoole (a1) and C. Arme (a1)

Abstract

The interaction between Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda)and pronephric lymphocytes of carp, Cyprinus carpio L. was studied by examining proliferation of lymphocytes isolated from both naïve fish and fish injected intraperitoneally with cestode extract. Lymphocytes from naïve hosts were stimulated to proliferate in the presence of the extract depending upon the extract protein concentrations; lower concentrations (0.01–0.05μg/ml) induced the greatest response, and immunosuppression occurred at higher concentrations. Significant differences were noted in fish that received intraperitoneal injections of parasite extracts. Five days post-injection, lymphocyte proliferation was significantly greater in these individuals compared with sham injected or untreated controls. This difference was reduced at 10 days post-injection, although the response was dependent on the concentration of the parasite extract. The possible significance of the observed stimulation/suppression of lymphocyte activity to establishment of the parasite in the wild is discussed.

Copyright

Corresponding author

Author for correspondence.

References

Hide All
Bruno, D.W. & Munro, A.L.S. (1986) Haematological assessment of rainbow trout, Salmo gairdneri Richardson, and Atlantic salmon, Salmo salar L., infected with Renibacterium salmoninarum. Journal of Fish Diseases 9, 195204
Caspi, R.R., Shahrabani, R., Kehati-Dan, T. & Avtalion, R.R. (1984) Heterogeneity of mitogen-responsive lymphocyte in carp (Cyprinus carpio). Development and Comparative Immunology 8, 6170.
Hoole, D. (1994) Tapeworm infections in fish: past and future problems, pp. 119140in Pike, A.W. & Lewis, J.W. (Eds) Parasitic diseases of fish. Dyfed, Samara Publishing Limited.
Judson, D.G., Dixon, J.B. & Skerritt, G.C. (1987) Occurrence and biochemical characteristics of cestode mitogens. Parasitology 94, 151160.
Koumans-van Diepen, J.C.E., Harmsen, E.G.M. & Roubout, J.H.W.M. (1994) Immunocytochemical analysis of mitogen response of carp (Cyprinus carpio L.) peripheral blood leucocytes. Veterinary Immunology and Immunopathology 42, 209219.
Liao, H. & Shih, L. (1956) Contribution to the biology and control of Bothriocephalus gowkongensis Yeh, a tapeworm parasitic in the young grass carp (Ctenopharyngodon idellus C. & V). Acta Hydrobiologica Sinica 2, 129185.
Pike, A.W. & Lewis, J.W. (1994) Parasitic diseases of fish. Dyfed, Samara Publishing Limited.
Pourreau, C.N., Koopman, M.B.H., Hendriks, G.F.R., Evenberg, D. & van Muiswinkel, W.B. (1987) Modulation of the mitogenic PHA response of carp, Cyprinus carpio L., by extracellular products of Aeromonas salmonicida. Journal of fish Biology 31 (Supplement A), 133143.
Richards, D.T., Hoole, D., Lewis, J.W., Ewens, E. & Arme, C.(1994) Changes in the cellular composition of the spleen and pronephros of carp Cyprinus carpio infected with the blood fluke Sanguinicola inermis (Trematoda: Sanguinicolidae). Diseases of Aquatic Organisms 19, 173179.
Richards, D.T., Hoole, D., Lewis, J.W., Ewens, E. & Arme, C. (1996) Stimulation of carp (Cyprinus carpio L.) lymphocytes by the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae). Diseases of Aquatic Organisms (in press).
Rijkers, G.T., Wiegerinck, J.A.M., van Oosterom, R. & van Muiswinkel, W.B. (1980) The immune system of cyprinid fish. Kinetics and temperature dependence of antibody-producing cells in carp (Cyprinus carpio). Immunology 41, 9197.
Rowley, A.F., Hunt, T.C., Page, M. & Wainwaring, G. (1988) Fish. pp. 1975in Rowley, A.F. & Ratcliffe, N.A. (Eds) Vertebrate blood cells. Cambridge, Cambridge University Press.
Secombes, C.J. & Manning, M.J. (1982) Histological changes in lymphoid organs of carp following injection of soluble or particulate antigens. Developmental and Veterinary Immunology (Supplement) 2, 5358.
Secombes, C.J., Manning, M.J. & Ellis, A.E. (1982) The effect of primary and secondary immunization on the lymphoid tissues of the carp, Cyprinus carpio L. Journal of Experimental Zoology 220, 277287.
Stolen, J.S., Fletcher, T.C., Anderson, D.A., Roberson, B.S. & van Muiswinkel, W.B. (1990) Techniques in fish immunology. Fish Immunology Technical Communications 1. Fair Haven, SOS Publications.
Tatner, M.F. (1990) Antigen-induced blastogenesis of salmon, Salmo salar L., head kidney leucocytes to modified Aeromonas salmonicida antigens: a preliminary evaluation method for potential vaccine antigens. Journal of Fish Biology 36, 731739.
Taverne, J. (1989) Immunity to protozoa and worms, pp. 17.117.21in Roitt, I., Brostoff, J. & Male, D. (Eds) Immunology (2nd ed), London, Gower.
Taylor, M.J. & Hoole, D. (1994) Modulation of fish lymphocyte proliferation by extracts and isolated proteinase inhibitors of Ligula intestinalis (Cestoda). Fish and Shellfish Immunology 4, 221230.
Williams, M.A. & Hoole, D. (1992) Antibody response of the fish Rutilus rutilus to the metacestode of Ligula intestinalis. Diseases of Aquatic Organisms 12, 8389.
Woo, P.T.K. (1987) Cryptobia and cryptobiasis in fishes. Advances in Parasitology 26, 199224.

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed