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Presence of a conserved domain of GATA transcription factors in Echinococcus granulosus

Published online by Cambridge University Press:  05 June 2009

B. Garat ,
P. Esperón ,
C. Martínez ,
C. Robello  and
R. Ehrlich
B. Garat
Affiliation:
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11200, Uruguay
P. Esperón
Affiliation:
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11200, Uruguay Cátedra de Biología, Facultad de Química, Universidad de la Republica, Montevideo 11200, Uruguay
C. Martínez
Affiliation:
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11200, Uruguay
C. Robello
Affiliation:
Departamento de Bioquímica, Facultad de Medicina, Universidad de la Republica, Montevideo 11200, Uruguay
R. Ehrlich
Affiliation:
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11200, Uruguay
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Abstract

In order to characterize GATA transcription factors in Echinococcus granulosus, a PCR-based cloning strategy was developed. Degenerate oligonucleotides were designed for the most conserved sequence in GATA proteins that include 20 amino acids of the zinc domain. A 60 bp fragment was isolated that had high homology among this sequence and those reported in other species. An analogous sequence was obtained by performing the same procedure with DNA from the free living platyhelminth Dugesia tigrina. High stringency Southern blotting experiments confirmed the presence of this sequence in the parasite genome.

Type
Research Note
Information
Journal of Helminthology , Volume 71 , Issue 4 , December 1997 , pp. 355 - 358
Copyright
Copyright © Cambridge University Press 1997

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