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The predatory capability of three nematophagous fungi in the control of Haemonchus contortus infective larvae in ovine faeces

Published online by Cambridge University Press:  12 April 2024

J. Flores-Crespo ,
D. Herrera-Rodríguez ,
P. Mendoza de Gives ,
E. Liébano-Hernández ,
V.M. Vázquez-Prats  and
M.E. López-Arellano
J. Flores-Crespo
Affiliation:
Proyecto Control Biológico, Centro Nacional de Investigaciones Disciplinarias en Parasitología Veterinaria (CENID-PAVET) INIFAP, Km. 11.5 Carr. Fed. Cuernavaca-Cuautla, Col. Progreso, Municipio de Jiutepec, Estado de Morelos 62550, México
D. Herrera-Rodríguez
Affiliation:
Proyecto Control Biológico, Centro Nacional de Investigaciones Disciplinarias en Parasitología Veterinaria (CENID-PAVET) INIFAP, Km. 11.5 Carr. Fed. Cuernavaca-Cuautla, Col. Progreso, Municipio de Jiutepec, Estado de Morelos 62550, México
P. Mendoza de Gives*
Affiliation:
Proyecto Control Biológico, Centro Nacional de Investigaciones Disciplinarias en Parasitología Veterinaria (CENID-PAVET) INIFAP, Km. 11.5 Carr. Fed. Cuernavaca-Cuautla, Col. Progreso, Municipio de Jiutepec, Estado de Morelos 62550, México
E. Liébano-Hernández
Affiliation:
Proyecto Control Biológico, Centro Nacional de Investigaciones Disciplinarias en Parasitología Veterinaria (CENID-PAVET) INIFAP, Km. 11.5 Carr. Fed. Cuernavaca-Cuautla, Col. Progreso, Municipio de Jiutepec, Estado de Morelos 62550, México
V.M. Vázquez-Prats
Affiliation:
Proyecto Control Biológico, Centro Nacional de Investigaciones Disciplinarias en Parasitología Veterinaria (CENID-PAVET) INIFAP, Km. 11.5 Carr. Fed. Cuernavaca-Cuautla, Col. Progreso, Municipio de Jiutepec, Estado de Morelos 62550, México
M.E. López-Arellano
Affiliation:
Proyecto Control Biológico, Centro Nacional de Investigaciones Disciplinarias en Parasitología Veterinaria (CENID-PAVET) INIFAP, Km. 11.5 Carr. Fed. Cuernavaca-Cuautla, Col. Progreso, Municipio de Jiutepec, Estado de Morelos 62550, México
*
*Fax: 01777 320 55 44 E-mail: pedromdgives@hotmail.com
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Abstract

The effect of oral administration of three different nematode-trapping fungi, in aqueous suspension containing either Dactylaria sp. or Arthrobotrys oligospora conidia or Duddingtonia flagrans chlamydospores, on the number of Haemonchus contortus infective larvae in sheep faeces, was evaluated. The three selected species of fungi produce three-dimensional adhesive nets in the presence of nematodes. Sixteen Creole sheep were divided into four groups of four animals each. Groups 1 and 2 were orally drenched with a suspension containing 2×107 conidia of either A. oligospora or Dactylaria sp. Group 3, received a similar treatment, with D. flagrans chlamydospores, instead of conidia, being administered, at the same dose. Group 4 acted as control, without any fungi. Faecal samples were collected directly from the rectum of each sheep and faecal cultures were prepared and incubated at 15 and 21 days. Larvae were recovered from faecal cultures and counted. The highest reduction of the nematode population occurred in the D. flagrans group, reaching reductions of 96.3% and 91.4% in individual samplings in plates incubated for 15 and 21 days, respectively. Arthrobotrys oligospora showed moderate reductions in the faecal larval population, ranging between 25–64% at 15 days incubation. In general, Dactylaria sp., was less efficient in its trapping ability. Despite the inconsistent results with Dactylaria sp., reduction percentages of 73.4% and 80.7% were recorded in individual samplings during the first and second days, in plates incubated for 15 days. Duddingtonia flagrans, was shown to be a potential biological control agent of H. contortus infective larvae.

Type
Review Article
Information
Journal of Helminthology , Volume 77 , Issue 4 , December 2003 , pp. 297 - 303
Copyright
Copyright © Cambridge University Press 2003

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References

Araújo, J.V., Santos, M.A., Ferraz, S. & Maia, A.S. (1993) Antagonistic effect of predaceous Arthrobotrys fungi on infective Haemonchus placei larvae. Journal of Helminthology 7, 136–138.CrossRefGoogle Scholar
Barron, G.L. (1977) The nematode destroying fungi. 140 pp. Guelph, Canada, Canadian Biological Publications Ltd. Google Scholar
Bisset, S.A., Vlasoff, C., West, J. & Morrison, L. (1997) Epidemiology of nematodosis in Romney lambs selectively bred for resistance or susceptibility to nematode infection. Veterinary Parasitology 70, 255–259.CrossRefGoogle ScholarPubMed
Charleston, T. (1994) Control of gastrointestinal parasites in beef production systems. Veterinary Continuing Education, Massey University, No. 159, 157–174.Google Scholar
Cheville, F.N. (1988) Introduction to veterinary pathology. Ames Iowa, University Press.Google Scholar
Faedo, M., Larsen, M. & Waller, J.P. (1997) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: comparison between Australian isolates of Arthrobotrys spp. and Duddingtonia flagrans . Veterinary Parasitology 72, 149–155.CrossRefGoogle ScholarPubMed
Faedo, M., Barnes, E.H., Dobson, R.J. & Waller, J.P. (1998) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: pasture plot study with Duddingtonia flagrans . Veterinary Parasitology 76, 129–135.CrossRefGoogle ScholarPubMed
Flores Crespo, J., Herrera Rodríguez, D., Vázquez Prats, V., Flores Crespo, R., Liébano Hernández, E. & Mendoza-De Gives, P. (1999) Acción depredadora in vitro de ocho aislados de hongos contra el nematodo Panagrellus redivivus . Revista Latinoamericana de Microbiología 41, 239–244.Google ScholarPubMed
Flores-Crespo, J., Mendoza-de Gives, P., Herrera Rodríguez, D., Liébano Hernández, E., Vázquez Prats, V. & Clark, S.J. (2001) Comparison of the use of two nematode-trapping fungi in the control of Haemonchus contortus infective larvae in ovine faeces. International Journal of Nematology 11, 253–259.Google Scholar
Githigia, S.M., Kimoro, C.O., Mwangi, D.M. & Gichanga, E.J. (1995) Prevalence and economic significance of Oesophagostomum and other helminths: survey in selected abattoirs around Nairobi, Kenya. Bulletin of Animal Health and Production in Africa 43, 29–33.Google Scholar
González Cruz, M.E., Mendoza de Gives, P. & Quiróz Romero, H. (1998) Comparison of the trapping ability of Arthrobotrys robusta and Monacrosporium gephyropagum on infective larvae of Strongyloides papillosus . Journal of Helminthology 72, 209–214.CrossRefGoogle ScholarPubMed
Grønvold, J., Nansen, P., Henriksen, S.A., Thylin, J. & Wolstrup, J. (1988) The capability of the predacious fungus Arthrobotrys oligospora (Hyphomycetales) to reduce numbers of infective larvae of Ostertagia ostertagi (Trichostrongylidae) in cow pats and herbage during the grazing season in Denmark. Journal of Helminthology 62, 271–280.CrossRefGoogle ScholarPubMed
Grønvold, J., Henriksen, S.A., Nansen, P., Wolstrup, J. & Thylin, J. (1989) Attempts to control infection with Ostertagia ostertagi (Trichostrongylidae) in grazing calves by adding mycelium of the nematode-trapping predacious fungus Arthrobotrys oligospora (Hyphomycetales) to cow pats. Journal of Helminthology 63, 115–126.CrossRefGoogle Scholar
Grønvold, J., Wolstrup, J., Nansen, P., Henriksen, S.A., Larsen, M. & Bresciani, J. (1993) Biological control of nematode parasites in cattle with nematode-trapping fungi: a survey of Danish studies. Veterinary Parasitology 48, 311–325.CrossRefGoogle ScholarPubMed
Gruner, L., Peloille, M., Sauve, C. & Cortet, J. (1985) Survie et conservation de 1'activité pedratice vis-à-vis de nematodes Trichostrongylides après ingestion par des ovins the trios hyphomycetes predateurs. Comptes Rendus des Seances de la Societé de Biologie, París v. 300, Serie III, n, 14, 525–528.Google Scholar
Hurley, D., Aguilar, A., Garibay, J. & Landeros, J. (1981) Técnicas de diseño experimental. Pruebas a posteriori. Diferencia mínima significativa. Prueba de Tukey. México D. F. Centro de Investigación y Estudios Avanzados, Universidad Nacional Autónoma de México. pp. 37–53.Google Scholar
Larsen, M. (2000) Prospects for controlling animal parasitic nematodes by predacious microfungi. Parasitology 120, S121–S131.CrossRefGoogle Scholar
Larsen, M., Wolstrup, J., Henriksen, S.A., Dackman, C., Grønvold, J. & Nansen, P. (1991) In vitro stress selection of nematophagous fungi for biocontrol of parasitic nematodes in ruminants. Journal of Helminthology 65, 193–200.CrossRefGoogle ScholarPubMed
Larsen, M., Wolstrup, J., Henriksen, S.A., Grønvold, J. & Nansen, P. (1992) In vivo passage through calves of nematophagous fungi selected for biocontrol of parasitic nematodes in ruminants. Journal of Helminthology 66, 137–141.CrossRefGoogle Scholar
Larsen, M., Faedo, M. & Waller, P. (1994) The potential of nematophagous fungi to the control the free-living stages of nematode parasites of sheep: survey for the presence of fungi in fresh faeces of grazing livestock in Australia. Veterinary Parasitology 53, 275–281.CrossRefGoogle Scholar
Larsen, M., Faedo, M., Waller, P. & Hennesy, D.R. (1998) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: studies with Duddingtonia flagrans . Veterinary Parasitology 76, 121–128.CrossRefGoogle ScholarPubMed
Liébano-Hernández, E. (1989) Cultivo e identificación larvaria de nematodos del tracto gastroentérico. pp. 40–71 in Ruelas, R.C. & Garfias, R.B. (Eds) Diagnóstico de helmintos y hemoparásitos de rumiantes. Asociación Mexicana de Parasitología Veterinaria, A.C., México, D.F. Google Scholar
Llerandi-Juarez, R.D. & Mendoza-de Gives, P. (1998) Resistance of nematophagous fungi chlamydospores to the digestive processes of sheep in Mexico. Journal of Helminthology 72, 155–158.CrossRefGoogle Scholar
Medway, W., Prier, E.J. & Wilkinson, S.J. (1980) Patología clínica veterinaria. Unión Tipográfica Editorial México. pp. 224–230.Google Scholar
Mendoza-de Gives, P., Zavaleta-Mejia, E., Quiroz-Romero, H., Herrera-Rodriguez, D. & Perdomo-Roldan, F. (1992) Interaction between the nematode-destroying fungus Arthrobotrys robusta (Hyphomycetales) and Haemonchus contortus infective larvae in vitro . Veterinary Parasitology 41, 101–107.CrossRefGoogle ScholarPubMed
Mendoza-de Gives, P., Flores Crespo, J., Herrera Rodríguez, D., Vázquez Prats, V.M., Liébano-Hernández, E. & Ontiveros Fernández, G.E. (1998) Biological control of Haemonchus contortus infective larvae in ovine faeces by administering an oral suspension of Duddingtonia flagrans chlamydospores to sheep. Journal of Helminthology 72, 347–347.CrossRefGoogle ScholarPubMed
Mendoza-De Gives, P., Davies, G.K., Clark, S.J. & Behnke, J.M. (1999) Predatory behaviour of trapping fungi against srf mutants of Caenorhabditis elegans and different plant and animal parasitic nematodes. Parasitology 119, 95–104.CrossRefGoogle ScholarPubMed
Nansen, P. (1986) Induction losses and control of helminths in ruminants of temperate regions. International Journal for Parasitology 17, 425–434.CrossRefGoogle Scholar
Nansen, P. (1987) Ruminant nematodiasis in temperate climates: some production losses associated with intensive husbandry methods. pp. 69–72 in Kenning, H.D.W. & Guerrero, J. (Eds) The economic impact of parasitism in cattle. Proceedings of the MSD AGVET Symposium. 19 August, 1987, in association with the XXIII World Veterinary Congress, Montreal, Quebec, Canada.Google Scholar
Pryadko, E.I. & Osipov, P.P. (1986) Trials of nematophagous fungi in field conditions. Biologicheskaya 1, 30–33.Google Scholar
Pryadko, E.I., Shol, V.A., Isabaev, M.I. & Shaltaeva, K.B. (1985) Nematophagous fungi as a biological control agent of nematode infections in farm animals, pp. 186–201 in Gvozdev, E.V. (Ed.) Gel'minty zhivotnykh v ekosystemakh Kazakhstana. Alma-Ata, USSR, “Nauka” Kazakhskoi SSR.Google Scholar
Sauer, S. (1996) Epidemiologie und klinsche auswirkungen von infektionen mit Magen-Darm-Strongyliden bei Schaflammern in Koppelhaltung. 151 pp. Inaugural-Disertation, Fachbereich Veterinarmedizin., Justus-Liebig Universitat, Giessen, Germany.Google Scholar
Síntesis geográfica de Morelos (1981) Coordinación de Servicios Nacionales de Estadística, Geografía e Informática (INEGI). Secreatría de Programación y Presupuesto, México, D.F. Google Scholar
Soulsby, E.J.L. (1987) Parasitología y enfermedades parasitarias de los animales domésticos. Ed. Interamericana 1a edición.Google Scholar
Ulloa, M. & Hanlin, R. (1978) Atlas de micología básica. México, D.F., Editorial Concepto.Google Scholar
Waller, P.J. (1993) Nematophagous fungi prospective biological control agents of animal parasitic nematodes. Parasitology Today 9, 529–531.CrossRefGoogle ScholarPubMed
Waller, P.J. & Faedo, M. (1993) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: screening studies. Veterinary Parasitology 49, 285–297.CrossRefGoogle ScholarPubMed