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Performance of the commercially available SERION ELISA classic Echinococcus IgG test for the detection of cystic echinococcosis in clinical practice

  • M.J. Sarink (a1), R. Koelewijn (a1), B.C.G.C. Slingerland (a1), A.G.M. Tielens (a1) (a2), P.J.J. van Genderen (a3) and J.J. van Hellemond (a1)...

Abstract

Diagnosis of cystic echinococcosis (CE) is at present mainly based on imaging techniques. Serology has a complementary role, partly due to the small number of standardized and commercially available assays. Therefore we examined the clinical performance of the SERION ELISA classic Echinococcus IgG test. Using 10 U/ml as a cut-off point, and serum samples from 50 CE patients and 105 healthy controls, the sensitivity and specificity were 98.0% and 96.2%, respectively. If patients with other infectious diseases were used as negative controls, the specificity decreased to 76.9%, which causes poor positive predictive values. However, if results between 10 and 15 U/ml are classified as indecisive, the specificity of positive results (≥15 U/ml) increased to 92.5% without greatly affecting the sensitivity (92.0%). Using this approach in combination with imaging studies, the SERION ELISA classic Echinococcosis IgG test can be a useful aid in the diagnosis of CE.

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Copyright

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

Author for correspondence: J.J. van Hellemond, Fax.: + 31 10 703 3875, E-mail: j.vanhellemond@erasmusmc.nl

References

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Ammann, RW and Eckert, J (1996) Cestodes. Echinococcus. Gastroenterology Clinics of North America 25, 655689.
Brunetti, E et al. (2010) Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Tropica 114, 116.
Budke, CM, Deplazes, P and Torgerson, PR (2006) Global socioeconomic impact of cystic echinococcosis. Emerging Infectious Diseases 12, 296303.
Del Carpio, M et al. (2012) Early diagnosis, treatment and follow-up of cystic echinococcosis in remote rural areas in Patagonia: impact of ultrasound training of non-specialists. PLoS Neglected Tropical Diseases 6, e1444.
Eckert, J et al. (2001) WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. World Organisation for Animal Health (Office International des Epizooties), Paris, France, and World Health Organization, Geneva, Switzerland.
Grosso, G et al. (2012) Worldwide epidemiology of liver hydatidosis including the Mediterranean area. World Journal of Gastroenterology 18, 14251437.
Kalantari, E et al. (2010) Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diagnosing hydatidosis. Parasitology Research 106, 847851.
Lissandrin, R et al. (2016) Factors influencing the serological response in hepatic Echinococcus granulosus infection. The American Journal of Tropical Medicine and Hygiene 94, 166171.
Manzano-Roman, R et al. (2015) Serological diagnosis and follow-up of human cystic echinococcosis: a new hope for the future? BioMed Research International 2015, 428205.
Marinova, I et al. (2011) Quantitative assessment of serum-specific IgE in the diagnosis of human cystic echinococcosis. Parasite Immunology 33, 371376.
Pagnozzi, D et al. (2016) Diagnostic accuracy of antigen 5-based ELISAs for human cystic echinococcosis. PLoS Neglected Tropical Diseases 10, e0004585.
Polat, P et al. (2003) Hydatid disease from head to toe. RadioGraphics 23, 475494.
Tamarozzi, F et al. (2013) Recombinant AgB8/1 ELISA test vs. commercially available IgG ELISA test in the diagnosis of cystic echinococcosis. Parasite Immunology 35, 433440.
Tamarozzi, F et al. (2016) Comparison of the diagnostic accuracy of three rapid tests for the serodiagnosis of hepatic cystic echinococcosis in humans. PLoS Neglected Tropical Diseases 10, e0004444.
Tamer, GS et al. (2015) Evaluation of immunochromatographic test for the detection of antibodies against Echinococcosis granulosus. Medical Science Monitor 21, 12191222.
Torgerson, PR and Deplazes, P (2009) Echinococcosis: diagnosis and diagnostic interpretation in population studies. Trends in Parasitology 25, 164170.
Torgerson, PR et al. (2015) World Health Organization estimates of the global and regional disease burden of 11 foodborne parasitic diseases, 2010: a data synthesis. PLoS Medicine 12, e1001920.
van Doorn, HR et al. (2007) Reliable serodiagnosis of imported cystic echinococcosis with a commercial indirect hemagglutination assay. Diagnostic Microbiology and Infectious Disease 57, 409412.
WHO Informal Working Group (2003) International classification of ultrasound images in cystic echinococcosis for application in clinical and field epidemiological settings. Acta Tropica 85, 253261.

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Performance of the commercially available SERION ELISA classic Echinococcus IgG test for the detection of cystic echinococcosis in clinical practice

  • M.J. Sarink (a1), R. Koelewijn (a1), B.C.G.C. Slingerland (a1), A.G.M. Tielens (a1) (a2), P.J.J. van Genderen (a3) and J.J. van Hellemond (a1)...

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