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Molecular genotyping of Echinococcus granulosus from dromedaries (Camelus dromedarius) in eastern Iran

Published online by Cambridge University Press:  10 September 2013

E. Moghaddas ,
H. Borji ,
A. Naghibi ,
P. Shayan  and
G.R. Razmi
E. Moghaddas
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
H. Borji*
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
A. Naghibi
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
P. Shayan
Affiliation:
Department of Parasitology, School of Veterinary Medicine, Tehran University, Tehran, Iran
G.R. Razmi
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
*
*E-mail: hborji@um.ac.ir
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Abstract

With the aim of genotyping Echinococcus granulosus cysts found in Iranian dromedaries (Camelus dromedarius), 50 cysts of E. granulosus were collected from five geographical regions in Iran. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1). Morphological criteria using rostellar hook dimensions were also undertaken. The present results have shown that 27 out of 50 E. granulosus cysts (54%) were determined as the G1 strain, and the other (46%) were determined as the G6 strain. The molecular analysis of the ITS1 region of ribosomal DNA corresponded with the morphological findings. Because of its recognized infectivity in humans, the G1 genotype is a direct threat to human health and its presence in Iranian dromedaries is of urgent public health importance.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 1 , January 2015 , pp. 100 - 104
Copyright
Copyright © Cambridge University Press 2013 

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