Hostname: page-component-76fb5796d-9pm4c Total loading time: 0 Render date: 2024-04-25T13:43:05.924Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization of Moniezia sichuanensis in captive musk deer (Moschus berezovskii)

Published online by Cambridge University Press:  19 July 2017

J. Xu ,
H. Lin ,
J. Chen ,
Y. Cai ,
W. Fu ,
H. Wang ,
X. Gu ,
W. Lai ,
X. Peng  and
G. Yang
J. Xu
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
H. Lin
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
J. Chen
Affiliation:
Sichuan Institute of Musk Deer Breeding, Dujiangyan 611830, China
Y. Cai
Affiliation:
Sichuan Institute of Musk Deer Breeding, Dujiangyan 611830, China
W. Fu
Affiliation:
Sichuan Institute of Musk Deer Breeding, Dujiangyan 611830, China
H. Wang
Affiliation:
Fengchun Pharmaceutical Co., Ltd, Chengdu 610081, China
X. Gu
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
W. Lai
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
X. Peng
Affiliation:
Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an 625014, China
G. Yang*
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
*
*E-mail: guangyou1963@aliyun.com
Get access Rights & Permissions [Opens in a new window]

Abstract

The musk deer (Moschus berezovskii) is an economically important species from which musk is extracted and used in perfumes and medicines. Cestodes (parasitic flatworms) of the genus Moniezia are important parasites that infect this endangered species and can cause high mortality in young deer. In 1982, Moniezia (S.) sichuanensis sp. nov. was described from a specimen obtained from wild musk deer. The new species was distinct from the other described species of Moniezia by the sawtooth-shaped interproglottidal glands, the thick vagina and the absence of a cirrus spine. In the present study, 12 cestodes collected from musk deer were examined morphologically and confirmed to be M. sichuanensis. Molecular characterization was performed by amplifying and comparing the internal transcribed spacer 1 (ITS1) and 5.8S rRNA gene (ITS1–5.8S) of ribosomal DNA with available sequences from other Moniezia species. The amplified sequences ranged from 761 to 764 bp and similarity ranged from 98.7–100%, compared to 67.8–92.4% with other Moniezia spp. Construction of a phylogenetic tree using the neighbour-joining method indicated that all 12 ITS1–5.8S sequences formed a single clade, confirming M. sichuanensis as a separate species. This study provides novel molecular insight into M. sichuanensis that could prove useful for future diagnosis and control of monieziasis in musk deer.

Type
Research Paper
Information
Journal of Helminthology , Volume 92 , Issue 4 , July 2018 , pp. 477 - 483
Copyright
Copyright © Cambridge University Press 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally to this work.

References

Bowles, J., Blair, D. & McManus, D. (1995) A molecular phylogeny of the genus Echinococcus. Parasitology 110, 317–328.Google Scholar
Cai, Y., Lin, H., Cheng, J., Wang, Y., Fu, W., Zhu, P., Wu, J. & Yang, G. (2016) Prevalence of endo-parasites in captive musk deer of China. Sichuan Journal of Zoology 35, 74–77.Google Scholar
Chilton, N.B., O'Callaghan, M.G., Beveridge, I. & Andrews, R.H. (2007) Genetic markers to distinguish Moniezia expansa from M. benedeni (Cestoda: Anoplocephalidae) and evidence of the existence of cryptic species in Australia. Parasitology Research 100, 1187–1192.CrossRefGoogle Scholar
Diop, G., Yanagida, T., Hailemariam, Z., Menkir, S., Nakao, M., Sako, Y., Ba, C.T. & Ito, A. (2015) Genetic characterization of Moniezia species in Senegal and Ethiopia. Parasitology International 64, 256–260.Google Scholar
Fazaeli, A., Carter, P. & Pennington, T. (2000) Intergenic spacer (IGS) polymorphism: a new genetic marker for differentiation of Toxoplasma gondii strains and Neospora caninum. Journal of Parasitology 86, 716–723.Google Scholar
Green, M.J.B. (1986) The distribution, status and conservation of the Himalayan musk deer Moschus chrysogaster. Biological Conservation 35, 347–375.Google Scholar
Li, X. & Jiang, X. (2014) Implication of musk deer (Moschus spp.) depletion from hunter reports and dung transect data in northwest Yunnan, China. Journal for Nature Conservation 22, 474–478.Google Scholar
Lin, Y. & He, Y. (1975) Studies on the development of Moniezia expansa (Rudolphi, 1810), its epidemiology and biology of its vectors. Journal of Xiamen University (Natural Science) (2), 91–123.Google Scholar
Liu, J., Wang, K. & Bo, X. (2008) Observation and comparison of reproductive organs morphology of Moniezia benedeni and Moniezia expansa. Chinese Journal of Veterinary Parasitology 16, 6–11.Google Scholar
Machida, M., Matsumura, A. & Watanabe, S. (1974) A rare cestode, Moniezia monardi (Anoplocephalidae), from the Japanese serow. Bulletin of the National Science Museum Tokyo 17, 157–161.Google Scholar
Nguyen, T., Le, Q., Huynh, V., Nguyen, S., Nguyen, T. & Vu-Khac, H. (2012) The development of PCR methodology for the identification of species of the tapeworm Moniezia from cattle, goats and sheep in central Vietnam. Journal of Helminthology 86, 426–429.Google Scholar
Ohtori, M., Mikiko, A. & Itagaki, T. (2015) Sequence differences in the internal transcribed spacer 1 and 5.8S ribosomal RNA among three Moniezia species isolated from ruminants in Japan. Journal of Veterinary Medical Science 77, 105–107.Google Scholar
Schmidt, G.D. (1986) CRC handbook of tapeworm identification. Boca Raton, Florida, CRC Press.Google Scholar
Shen, J. (2005) A list of parasites for livestock and poultry in Chinese supplement III. Chinese Journal of Animal Infectious Diseases 13, 16–19.Google Scholar
Sheng, H.L. & Liu, Z.X. (2007) The musk deer in China. Shanghai, The Shanghai Scientific & Technical Publishers.Google Scholar
Skrjabin, K. & Schulz, R. (1937) Helminthology. Moscow.Google Scholar
Smith, A.T., Xie, Y., Hoffmann, R.S., Lunde, D., MacKinnon, J., Wilson, D.E. & Wozencraft, W.C.E. (Eds) (2010) A guide to the mammals of China. Princeton, New Jersey, Princeton University Press.Google Scholar
Spassky, A. (1951) Anaplocaphalata cestodes of domestic and wild animals. Osnovy tsestodologii 1, 735.Google Scholar
Taylor, E.L. (1967) Moniezia, a genus of cestode worms, and the proposed reduction of its species to three. Proceedings of the US National Museum 74.Google Scholar
Wang, K., Hao, X. & Liu, S. (2010a) The summary of developing status and economic benefit analysis of Moschus berezovskii breeding in China. Chinese Journal of Animal Science 46, 14–17.Google Scholar
Wang, K., Liu, J. & Bo, X. (2010b) Comparison on the morphology of Moniezia benedeni and Moniezia expansa. Xinjiang Agricultural Sciences 47, 612–618.Google Scholar
Wang, Y. & Harris, R.B. (2008) Moschus anhuiensis, Moschus berezovskii, Moschus chrysogaster and Moschus fuscus. In IUCN (2013) IUCN Red List of threatened species. Available at www.iucnredlist.org (accessed 30 February 2013).Google Scholar
Wickström, L.M., Haukisalmi, V., Varis, S., Hantula, J. & Henttonen, H. (2005) Molecular phylogeny and systematics of anoplocephaline cestodes in rodents and lagomorphs. Systematic Parasitology 62, 83–99.Google Scholar
Wu, J. (1982) Study of Moniezia (S.) sichuanensis sp. nov. (Cestoda. Anoplocephalidae). Zoological Research S2, 48–51.Google Scholar
Yang, G. & Zhang, Z. (2013) Parasitic diseases of wildlife. pp. 526–538. Beijing, Science Press.Google Scholar
Yang, G., Chen, W., Cai, Y., Sha, G. & Chen, J. (2001a) The effectiveness of praziquantel in eliminating Moniezia sichuanensis from captive musk deer (Moschus berezovskii). Chinese Journal of Animal Infectious Diseases 9, 59–60.Google Scholar
Yang, G., Sha, G., Cai, Y., Chen, J. & Chen, W. (2001b) Pathogen morphology observation of Moniezia sichuanensis in captive musk deer (Moschus berezovskii). Livestock and Poultry Industry, 49.Google Scholar
Yang, G., Cai, Y., Sha, G., Chen, W. & Chen, J. (2002) The epidemiology of Moniezia sichuanensis in captive musk deer (Moschus berezovskii). Progress in Veterinary Medicine 23, 60–62.Google Scholar