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Molecular characterization of Anisakis pegreffii larvae in Pacific cod in Japan

Published online by Cambridge University Press:  12 April 2024

N. Abe ,
N. Ohya  and
R. Yanagiguchi
N. Abe*
Affiliation:
Department of Microbiology, Tennoji-ku, Osaka 543-0026, Japan
N. Ohya
Affiliation:
Nutrition College, Osaka City Institute of Public Health and Environmental Sciences, Tennoji-ku, Osaka 543-0026, Japan
R. Yanagiguchi
Affiliation:
Nutrition College, Osaka City Institute of Public Health and Environmental Sciences, Tennoji-ku, Osaka 543-0026, Japan
*
*Fax: +81 6 6772-0676 E-mail: n.abe@iphes.city.osaka.jp
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Abstract

It is now recognized that the morphospecies Anisakis simplex is not a single species but a complex composed of three sibling species, A. simplex sensu stricto, A. pegreffii and A. simplex C. In Japan, A. simplex-like larvae have been isolated from a variety of fish and humans, but the larvae collected have been identified as A. simplex by only light microscopy. Therefore, the epidemiology of the A. simplex complex, composed of three sibling species, is still unclear in Japan. In the present study, 26 A. simplex-like larval isolates were obtained from two Pacific cod landed in Hokkaido, Japan, and examined genetically by PCR–RFLP and direct sequencing of the ITS region of rDNA. Among the 26 isolates, 24 were identified as A. simplex sensu stricto, the other two as A. pegreffii. The present study is the first to confirm the distribution of A. pegreffii in Japan, and to detect A. pegreffii larvae in Pacific cod.

Type
Review Article
Information
Journal of Helminthology , Volume 79 , Issue 4 , December 2005 , pp. 303 - 306
Copyright
Copyright © Cambridge University Press 2005

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References

Abollo, E., Paggi, L., Pascual, S. & D'Amelio, S. (2003) Occurrence of recombinant genotypes of Anisakis simplex, s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry. Infection, Genetics and Evolution 3, 175–181.CrossRefGoogle Scholar
D'Amelio, S., Mathiopoulos, K.D., Brandonisio, O., Lucarelli, G., Doronzo, F. & Paggi, L. (1999) Diagnosis of a case of gastric anisakidosis by PCR-based restriction fragment length polymorphism analysis. Parassitologia 41, 591–593.Google ScholarPubMed
D'Amelio, S., Mathiopoulos, K.D., Santos, C.P., Pugachev, O.N., Webb, S.C., Picanço, M & Paggi, L. (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. International Journal for Parasitology 30, 223–226.CrossRefGoogle ScholarPubMed
Kato, K., Kagei, N., Hayashi, Y. & Ando, Y. (1992) Parasitological and epidemiological survey of anisakid larvae from sardines, Engraulis japonica, caught in the sea near Kamogawa City, Chiba Prefecture, Japan, where human anisakiasis prevailed. Japanese Journal of Parasitology 41, 425–430.Google Scholar
Koyama, T., Kobayashi, A., Kumada, M., Komiya, Y., Oshima, T., Kagei, N., Ishii, T. & Machida, M. (1969) Morphological and taxonomical studies on Anisakidae larvae found in marine fishes and squids. Japanese Journal of Parasitology 18, 466–487.Google Scholar
Mattiucci, S., Nascetti, G., Bullini, L., Orecchia, P. & Paggi, L. (1986) Genetic structure of Anisakis physeteris, and its differentiation from the Anisakis simplex complex (Ascaridida: Anisakidae). Parasitology 93, 383–387.CrossRefGoogle ScholarPubMed
Mattiucci, S., Nascetti, G., Cianchi, R., Paggi, L., Arduino, P., Margolis, L., Brattey, J., Webb, S., D'Amelio, S., Orecchia, P. Bullini, L. (1997) Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae). Journal of Parasitology 83, 401–416.CrossRefGoogle ScholarPubMed
Nascetti, G. Paggi, L. Orecchia, P. Smith, J.W., Mattiucci, S. & Bullini, L. (1986) Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from the Mediterranean and North-East Atlantic. International Journal for Parasitology 16, 633–640.CrossRefGoogle ScholarPubMed
National Institute of Infectious Diseases and Tuberculosis and Infectious Diseases Control Division, Ministry of Health, Labour and Welfare (2004) Foodborne helminthiases as emerging diseases in Japan. Infectious Agents Surveillance Report 25, 114–115.Google Scholar
Orecchia, P., Paggi, L., Mattiucci, S., Smith, J.W., Nascetti, G. & Bullini, L. (1986) Electrophoretic identification of larvae and adults of Anisakis (Ascaridida: Anisakidae). Journal of Helminthology 60, 331–339.CrossRefGoogle ScholarPubMed
Oshima, T. & Kliks, M. (1987) Effects of marine mammal parasites on human health. International Journal for Parasitology 17, 415–421.Google ScholarPubMed
Paggi, L., Mattiucci, S., Ishikura, H., Kikuchi, K., Sato, N., Nascetti, G., Cianchi, R. & Bullini, L. (1998 a) Molecular genetics in anisakid nematodes from the Pacific Boreal region. 83–107 in Ishikura, H., Aikawa, M., Itakura, H. & Kikuchi, K. (Eds) Host response to international parasitic zoonoses. Tokyo Springer Verlag.CrossRefGoogle Scholar
Paggi, L., Nascetti, G., Webb, S.C., Mattiucci, S., Cianchi, R. & Bullini, L. (1998b) A new species of Anisakis Dujardin, 1845 (Nematoda, Anisakidae) from beaked whales (Ziphiidae): allozyme and morphological evidence. Systematic Parasitology 40, 161–174.CrossRefGoogle Scholar
Sakanari, J.A. & McKerrow, J.H. (1989) Anisakiasis. Clinical Microbiology Review 2, 278–284.CrossRefGoogle ScholarPubMed
Uchida, A., Kawakami, Y. & Murata, Y. (1998) Infection of Anisakis larvae in fishes from Sagami and Suruga bays. Journal of the Japan Veterinary Medical Association 51, 525–527.CrossRefGoogle Scholar
Zhu, X., Gasser, R.B., Podolska, M. & Chilton, N.B. (1998) Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International Journal for Parasitology 28, 1911–1921.CrossRefGoogle ScholarPubMed