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Maintenance of Helisoma trivolvis naturally infected with Echinostoma trivolvis in spring water at 4°C for 300 days

Published online by Cambridge University Press:  05 June 2009

K.A. Schmidt ,
B. Fried  and
A. Reddy
K.A. Schmidt
Affiliation:
Department of Biology, Lafayette College, Easton, PA 18042, USA
B. Fried*
Affiliation:
Department of Biology, Lafayette College, Easton, PA 18042, USA
A. Reddy
Affiliation:
Department of Biology, Lafayette College, Easton, PA 18042, USA
*
*Author for correspondence.
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Abstract

Helisoma trivolvis (Pennsylvania strain) snails naturally infected with Echinostoma trivolvis larvae were maintained for 10 months (300 days) at 4°C in artificial spring water to determine the effects of storage on parasite survival. Three of 20 snails were alive at 10 months and when isolated in spring water at 22−24°C released active cercariae. The mean number of cercariae released in 2 h from each stored snail was significantly less than that from freshly collected snails. Infectivity of cercariae from stored hosts to experimentally infected, laboratory-raised Helisoma trivolvis (Colorado strain) snails was significantly less than that of cercariae from fresh hosts based on cyst recoveries in the Colorado strain of H. trivolvis within 24 h p.i. There was no significant difference in the redial number or the number of cercariae per redia in stored versus fresh snails. In conclusion, some reduction in cercarial emergence and decrease in cercarial infectivity to a second intermediate snail host occurred following storage of H. trivolvis naturally infected with E. trivolvis in ASW at 4°C for 10 months.

Type
Research Note
Information
Journal of Helminthology , Volume 72 , Issue 1 , March 1998 , pp. 91 - 92
Copyright
Copyright © Cambridge University Press 1998

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References

Fried, B. & Perkins, C. (1982) Survival of metacercariae of Echinostorna revolutum (Trematoda) in half-strength Locke's solution under refrigeration. Proceedings of the Helminthological Society of Washington 49, 153–154.Google Scholar
Griffiths, H.J. & Christensen, C.A. (1974) Further observations on the survival of metacercariae of Fascioloides magna in water at room temperature and under refrigeration. Journal of Parasitology 60, 335.CrossRefGoogle Scholar
Reddy, A. & Fried, B. (1996) In vitro studies on intrspecific and interspecific chemical attraction in daughter rediae of Echinostoma trivolvis and E. caproni. International Journal for Parasitology 26, 1081–1085.CrossRefGoogle ScholarPubMed
Schmidt, K.A. & Fried, B. (1996a) Emergence of cercariae of Echinostoma trivolvis from Helisoma trivolvis under different conditions. Journal of Parasitology 82, 674–676.CrossRefGoogle ScholarPubMed
Schmidt, K.A. & Fried, B. (1996b) Experimental infection of Helisoma trivolvis (Colorado strain) snails with cercariae of Echinostoma trivolvis. International Journal for Parasitology 26, 287–289.CrossRefGoogle ScholarPubMed
Schmidt, K.A. & Fried, B. (1997) Prevalence of larval trematodes in Helisoma trivolvis (Gastropoda) from a farm pond in Northampton County, Pennsylvania with special emphasis on Echinostoma trivolvis (Trematoda) cercariae. Journal of the Helminthological Society of Washington 64, 157–159.Google Scholar
Ulmer, M.J. (1970) Notes on rearing of snails in the laboratory. pp. 143–144 in MacInnis, A. J. & Voge, M. (Eds) Experirnents and techniques in parasitology. San Francisco, W.H. Freeman and Co.Google Scholar