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Localization of a 24-kDa collagenase in the Gymnorhynchus gigas plerocercoid

Published online by Cambridge University Press:  12 April 2024

C. Vázquez-López ,
C. de Armas-Serra ,
J. Pérez-Serrano ,
C. Giménez-Pardo  and
F. Rodríguez-Caabeiro
C. Vázquez-López*
Affiliation:
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares, Ctra. Madrid-Barcelona km. 33.600, Madrid 28871, Spain
C. de Armas-Serra
Affiliation:
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares, Ctra. Madrid-Barcelona km. 33.600, Madrid 28871, Spain
J. Pérez-Serrano
Affiliation:
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares, Ctra. Madrid-Barcelona km. 33.600, Madrid 28871, Spain
C. Giménez-Pardo
Affiliation:
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares, Ctra. Madrid-Barcelona km. 33.600, Madrid 28871, Spain
F. Rodríguez-Caabeiro
Affiliation:
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares, Ctra. Madrid-Barcelona km. 33.600, Madrid 28871, Spain
*
*Fax: +34 1 885 46 63 E-mail: Celia.Vazquez@uni.alcala.es
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Abstract

A 24-kDa collagenase was localized in the Gymnorhynchus gigas plerocercoid immunohistochemically by peroxidase complex staining using polyclonal antibodies from NMRI mouse sera immunized with purified enzyme. Immunoreactivity was determined at different parts of the body (scolex, vesicle and caudal region) and mainly localized in microtriches and parenchymal tissues of the scolex and vesicle. These results, along with the absence of the enzyme in the plerocercoid excretion–secretion products, suggest that the 24-kDa collagenase is produced by parenchymal cells in the anterior region and transported to the outer regions of the worm It is possible that the enzyme plays an important role in degrading parasite tissues during the moulting process.

Type
Research Article
Information
Journal of Helminthology , Volume 74 , Issue 2 , June 2000 , pp. 183 - 187
Copyright
Copyright © Cambridge University Press 2000

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