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Isolation, purification and characterization of surface antigens of the bovine filarial parasite Setaria digitata for the immunodiagnosis of bancroftian filariasis

Published online by Cambridge University Press:  05 June 2009

J. Gerald Theodore  and
P. Kaliraj
J. Gerald Theodore
Affiliation:
Center for Biotechnology, Anna University, Madras 600 025, India
P. Kaliraj
Affiliation:
Center for Biotechnology, Anna University, Madras 600 025, India
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Abstract

The surface antigens of the bovine filarial parasite Setaria digitata were isolated by EDTA extraction and purified by affinity chromatography using sepharose bound human filarial (Wuchereria bancrofti) antibodies obtained from chronic human filarial sera. The purified and crude antigens were used in enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibodies in bancroftian filariasis. The purified antigen showed sensitive and specific reactions in ELISA for the detection of antibodies in filarial sera and showed least cross reactivity with other parasitic infections. The crude and purified antigens showed about 18 and 6 peptide bands respectively in SDS-PAGE and about 11 and 6 antigenic bands respectively in enzyme-linked immunoelectrotransfer blot (EITB). The purified antigen was observed to be glycoprotein in nature. It was possible to identify the stage-specific infection in human filariasis by using the crude and purified antigens in EITB.

Keywords

Setaria digitatasurface antigensimmunodiagnosisELISAWuchereria bancrofti
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 2 , June 1990 , pp. 105 - 114
Copyright
Copyright © Cambridge University Press 1990

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