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Investigation of strategies with potential for producing a phosphorylcholine-free version of the filarial nematode immunomodulator, ES-62

Published online by Cambridge University Press:  12 April 2024

K.M. Houston ,
C.A. Egan ,
P. García  and
W. Harnett
K.M. Houston
Affiliation:
Department of Immunology, University of Strathclyde, Glasgow, G4 0NR, UK
C.A. Egan
Affiliation:
Department of Immunology, University of Strathclyde, Glasgow, G4 0NR, UK
P. García
Affiliation:
Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain
W. Harnett*
Affiliation:
Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain
*
*Fax: 0141 548 3427 Email: w.harnett@strath.ac.uk
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Abstract

Phosphorylcholine (PC) is found attached to N-type glycans of proteins secreted by filarial nematodes, where it appears to act as an immunomodulator. Based on information on the structure and biosynthesis of the PC-glycan of a major secreted protein, ES-62, strategies were designed with potential for preparing PC-free material to better understand the importance of PC in filarial nematode immunomodulation. The strategies involve either enzymatic removal of PC or inhibition of its attachment during ES-62 synthesis. No method tested was found to be 100% effective although ∼70% removal was obtained by culturing worms in Et18OCH3. Reasons for failure to obtain complete absence of PC moieties are discussed in relation to the structure and synthesis of PC-glycans and in addition PC-glycan biosynthesis is briefly commented on as a target for chemotherapy.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 3 , September 2006 , pp. 261 - 265
Copyright
Copyright © Cambridge University Press 2006

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