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Immunolocalization of a putative cuticular collagen protein in several developmental stages of Meloidogyne arenaria, Globodera pallida and G. rostochiensis

Published online by Cambridge University Press:  12 April 2024

I.M. de O. Abrantes  and
R.H.C. Curtis
I.M. de O. Abrantes
Affiliation:
Departamento de Zoologia e Instituto do Ambiente e Vida, Universidade de Coimbra, 3004–517 Coimbra, Portugal
R.H.C. Curtis*
Affiliation:
Plant Pathogen Interaction Division, IACR-Rothamsted, Harpenden, Herts, AL5 2JQ, UK
*
*Author for correspondence Fax: +44 (0) 1582 760981 Email: rosane.curtis@bbsrc.ac.uk
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Abstract

The monoclonal antibody IACR-CCNj.3d has previously been used to isolate a gene (gp-col-8) with strong similarity to cuticular collagen from a mixed stage Globodera pallida cDNA expression library. The antibody has also been shown to label specifically the amphidial canal of pre-parasitic second stage juveniles (J2) of several plant nematode species without any reactivity on the cuticular surface, indicating that this protein is either not present or is inaccessible on the cuticular surface. This paper investigates the cross-reactivity of Mab IACR-CCNj.3d with Meloidogyne arenaria and the localization of the putative collagen protein on the cuticular surface of parasitic stages in planta and on the cuticular surface of juveniles inside eggs. The antigen was shown to be present in all developmental stages of the two species of potato cyst nematodes and M. arenaria. The antibody bound strongly to the amphidial canal and hypodermis of pre-parasitic J2 and adult females. The antigen was present on the cuticular surface of the sausage-shaped J2 in planta and of first stage juveniles (J1) inside the eggs. The presence of collagen on the surface of the cuticle of moulting stages of plant parasitic nematodes has been observed for the first time. It is clear that this protein has a role in the construction of the cuticle of the first stage juveniles and parasitic second stage juveniles, during moulting inside the eggs and in the root tissue, respectively.

Type
Review Article
Information
Journal of Helminthology , Volume 76 , Issue 1 , March 2002 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 2002

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