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Host response of Nicotiana benthamiana to the parasitism of five populations of root-lesion nematode, Pratylenchus coffeae, from China

Published online by Cambridge University Press:  29 September 2023

M. Sun ,
W. Niu ,
L. Shi ,
Y. Lv ,
B. Fu ,
Y. Xia ,
H. Li ,
K. Wang  and
Y. Li Open the ORCID record for Y. Li [Opens in a new window]
M. Sun
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, P.R.China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450046, P.R.China
W. Niu
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, P.R.China
L. Shi
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, P.R.China
Y. Lv
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, P.R.China
B. Fu
Affiliation:
College of Tobacco Science, Henan Agricultural University, Zhengzhou 450046, P.R.China
Y. Xia
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, P.R.China State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, P.R.China
H. Li
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, P.R.China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450046, P.R.China
K. Wang*
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, P.R.China
Y. Li*
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, P.R.China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450046, P.R.China
*
Corresponding authors: K. Wang and Y. Li; Emails: scauwangke@126.com; liyuzhibao@henau.edu.cn
Corresponding authors: K. Wang and Y. Li; Emails: scauwangke@126.com; liyuzhibao@henau.edu.cn
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Abstract

In a recent survey of nematodes associated with tobacco in Shandong, China, the root-lesion nematode Pratylenchus coffeae was identified using a combination of morphology and molecular techniques. This nematode species is a serious parasite that damages a variety of plant species. The model plant benthi, Nicotiana benthamiana, is frequently used to study plant-disease interactions. However, it is not known whether this plant species is a host of P. coffeae. The objectives of this study were to evaluate the parasitism and pathogenicity of five populations of the root-lesion nematode P. coffeae on N. benthamiana. N. benthamiana seedlings with the same growth status were chosen and inoculated with 1,000 nematodes per pot. At 60 days after inoculation, the reproductive factors (Rf = final population densities (Pf)/initial population densities (Pi)) for P. coffeae in the rhizosphere of N. benthamiana were all more than 1, suggesting that N. benthamiana was a good host plant for P. coffeae. Nicotiana. benthamiana infected by P. coffeae showed weak growth, decreased tillering, high root reduction, and noticeable brown spots on the roots. Thus, we determined that the model plant N. benthamiana can be used to study plant-P. coffeae interactions.

Keywords

Nicotiana benthamianaRoot-lesion nematodeidentificationparasitismpathogenicity
Type
Research Paper
Information
Journal of Helminthology , Volume 97 , 2023 , e73
Copyright
© The Author(s), 2023. Published by Cambridge University Press

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