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Genetic characterization of human-derived hydatid fluid based on mitochondrial gene sequencing in individuals from northern and western China

  • M. Cao (a1), K. Chen (a1), W. Li (a2), J. Ma (a1) (a2), Z. Xiao (a3), H. Wang (a4) and J. Gao (a1) (a2)...

Abstract

This study investigated Echinococcus genotypes in patients with hydatidosis that reside in Inner Mongolia, Tibet or Qinghai Province by partially sequencing the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) genes. Cyst fluids were collected from 23 patients with hydatidosis and DNA was extracted. Portions of the cox1 and nad1 genes were amplified and subsequently sequenced. Sequencing analysis determined that one of the isolates contained Echinococcus multilocularis, and the other 22 isolates contained E. granulosus sensu lato. The isolates were then further classified based on genotype, and E. granulosus sensu stricto (s.s.) G1 (n = 20), E. granulosus s.s. G3 (n = 1) and E. canadensis G6/7 (n = 1) were identified. Additionally, the sequences were concatenated (pcox1 + pnad1) and 11 haplotypes were identified among the E. granulosus s.s. isolates (G1 and G3), with a shared common haplotype (H1) identified. Overall, these findings provide further understanding of the genetic patterns of Echinococcus in western and northern China.

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Corresponding author

Authors for correspondence: H. Wang, E-mail: chfwhb@sina.com J. Gao, E-mail: golden_bright@hotmail.com

References

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