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Fasciola hepatica infections in cattle and the freshwater snail Galba truncatula from Dakhla Oasis, Egypt

Published online by Cambridge University Press:  06 February 2017

W.M. Arafa ,
A.I. Hassan ,
S.A.M. Snousi ,
Kh.M. El-Dakhly ,
P.J. Holman ,
T.M. Craig  and
S.M. Aboelhadid
W.M. Arafa
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine Beni-Suef University, Beni-Suef 62511, Egypt
A.I. Hassan
Affiliation:
Regional Animal Health Research Laboratory, Animal Health Research Institute, Dakhla, El-Wadi El-Gadid, Egypt
S.A.M. Snousi
Affiliation:
Regional Animal Health Research Laboratory, Animal Health Research Institute, Dakhla, El-Wadi El-Gadid, Egypt
Kh.M. El-Dakhly
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine Beni-Suef University, Beni-Suef 62511, Egypt
P.J. Holman
Affiliation:
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4467, USA
T.M. Craig
Affiliation:
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4467, USA
S.M. Aboelhadid*
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine Beni-Suef University, Beni-Suef 62511, Egypt
*
*Fax: 0020822327982 E-mail: drshawky2001@yahoo.com
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Abstract

Infection by Fasciola species was investigated in seven districts of Dakhla Oasis, Egypt, through abattoir inspection of cattle livers for adult worms and sedimentation of faecal samples from local cattle to detect Fasciola eggs. In addition, lymnaeid snails collected from the study area were examined microscopically for developmental stages of Fasciola spp. Abattoir inspection revealed that 51 out of 458 cattle livers (11.1%) contained adult flukes, which were identified morphologically as Fasciola hepatica. Examination of the cattle faecal samples revealed that 142 out of 503 (28.2%) contained Fasciola eggs. The collected snails, identified as Galba truncatula and Radix natalensis, showed larval stages of Fasciola in 71 out of 731 (9.7%) G. truncatula, while R. natalensis showed no infection. Specific duplex polymerase chain reaction (PCR) targeting the mitochondrial cox1 gene of F. hepatica and Fasciola gigantica was carried out on DNA extracted from pooled infected snails and adult worms. The F. hepatica size amplicon (1031 bp) was obtained from both the infected G. truncatula and the adult worms isolated from cattle livers from different districts. The amplicon sequences were identical to the published sequences of F. hepatica mitochondrial cox1 gene. In conclusion, the zoonotic importance of Fasciola infection and appropriate hygienic measures must be taken into consideration in Dakhla Oasis, Egypt.

Type
Research Papers
Information
Journal of Helminthology , Volume 92 , Issue 1 , January 2018 , pp. 56 - 63
Copyright
Copyright © Cambridge University Press 2017 

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