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Distribution of Anisakis larvae, identified by genetic markers, and their use for stock characterization of demersal and pelagic fish from European waters: an update

  • S. Mattiucci (a1), P. Abaunza (a2), S. Damiano (a1) (a3), A. Garcia (a4), M.N. Santos (a4) and G. Nascetti (a3)...


In the present paper, recent results obtained on the use of different distributions observed in larval species of Anisakis, genetically identified by means of allozyme markers, for stock characterization of demersal (Merluccius merluccius), small (Trachurus trachurus) and large pelagic (Xiphias gladius) finfish species in European waters, are reviewed and discussed. Several species of Anisakis were identified in the three fish hosts: A. simplex (s.s.), A. physeteris, A. typica, A. ziphidarum,A. pegreffii, A. brevispiculata and A. paggiae. Canonical discriminant analysis performed on all the samples of the three fish species collected in areas comprising their geographical range, according to the different species of Anisakis identified, showed distinct fish populations in European waters. In all the three fish hosts, the pattern of distribution of Anisakis larvae allowed discrimination of Mediterranean stocks from Atlantic stocks. In the case of swordfish, the possible existence of a southern Atlantic stock separated from a northern one is also suggested. Congruence and discordance with the population genetic data inferred from allozyme markers on the same samples of the three fish species are also discussed.


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Abollo, E., Gestal, C. & Pascual, S. (2001) Anisakis infestation in marine fish and cephalopods from Galician waters: an updated perspective. Parasitology Research 87, 492499.
Alvarado-Bremer, J.R., Mejuto, J., Gomez-Marquez, J., Pla-Zanny, C., Vinas, J., Marques, L., Hazin, F., Griffiths, M., Ely, B., Sanchez, B. & Greig, T.W. (2006) Genetic population structure of Atlantic swordfish: current status, controversies and future directions. ICCAT Swordfish Stock Structure Workshop. Heraklion, Crete, 13–15 March.
Begg, G. & Waldman, J.R. (1999) An holistic approach to fish stock identification. Fisheries Research 43, 3544.
Ben Salem, M. (1988) Régime alimentaire de Trachurus trachurus (Linnaeus, 1758) et de T. Mediterraneus (Steindachner, 1868), (Poissons, Téléostéens, Carangidae) de la province Atlantico-Mediterranéenne. Cybium 12, 247253.
Berland, B. (1961) Nematodes from some Norwegian marine fishes. Sarsia 2, 150.
Beverly-Burton, M., Nyman, O.L. & Pippy, J.H.C. (1977) The morphology, and some observations on the population genetics of Anisakis simplex larvae (Nematoda: Ascaridata) from fishes of the North Atlantic. Journal of the Fisheries Research Board of Canada 34, 105112.
Cimmaruta, R., Nascetti, G. & Bullini, L. (1996) Primi dati sulla struttura genetica di alcune specie di grandi pelagici del Mediterraneo. Biologia Marina Mediterranea 3, 400406.
Cimmaruta, R., Nascetti, G. & Bullini, L. (1998) Fisheries and gene diversity conservation: the case of swordfish and bluefin tuna in the Mediterranean Sea. Abstract VII INTECOL. Florence, Italy, 19–25 July.
Cimmaruta, R., Bondanelli, P. & Nascetti, G. (2005) Genetic structure and environmental heterogeneity in the European hake (Merluccius merluccius). Molecular Ecology 14, 25772591.
Cimmaruta, R., Paoletti, M., Bondanelli, P., Santos, M.N., Garcia, A. & Nascetti, G. (2006) Genetic structure of Mediterranean and Atlantic swordfish investigated by allozymes and RFLPs. ICCAT Swordfish Stock Structure Workshop. Heraklion, Crete, 13–15 March.
Cimmaruta, R., Bondanelli, P., Ruggi, A. & Nascetti, G. (2007) Genetic structure and temporal stability in horse mackerel (Trachurus trachurus). Fisheries Research. in press.
Cousteau, J.Y. & Paccalet, Y. (1986) Whales. New York, Arry N. Abrahms Inc.
D'Amelio, S., Mathiopoulos, K., Santos, C.P., Pugachev, O.N., Webb, S.C., Picanco, M. & Paggi, L. (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. International Journal for Parasitology 30, 223226.
Davey, J.T. (1971) A revision of the genus Anisakis Dujardin, 1845 (Nematoda: Ascaridida). Journal of Helminthology 45, 5172.
Koutulas, G., Mejuto, J., Kasapidi, P., Tserpes, G., Antoniou, A., Garcia-Cortes, B., Peristeraki, P., Oikonomaki, K. & Magoulas, A. (2006) Genetic structure of the swordfish Xiphias gladius stock in the Atlantic using microsatellite DNA analysis. ICCAT 2006 Swordfish Stock Structure Workshop. Heraklion, Crete, 13–15 March.
Legendre, P. & Legendre, L. (1998) Numerical ecology. 853 pp. Amsterdam, Elsevier.
Lopez, A., Santos, M.B., Pierce, G.J., Gonzales, A.F., Valeiras, X. & Guerra, A. (2001) Trends in stranding and by-catch marine mammals in north-west Spain during the 1990s. Journal of the Marine Biological Association of the United Kingdom 82, 513–521.
Lu, C.-P., Mejuto, J. & Yeh, S.-Y. (2006) Identification on Atlantic swordfish stock structure inferred by mitochondrial control region DNA sequence cheracters. ICCAT 2006 Swordfish Stock Structure Workshop. Heraklion, Crete, 13–15 March.
MacKenzie, K. (2002) Parasites as biological tags in population studies of marine organisms: an update. Parasitology 124, 153–163.
Martinez, W.L. & Martinez, A.R. (2005) Exploratory data with MATLAB. 405 pp. New York, Chapman & Hall/CRC.
McGarigal, K., Cushman, S. & Stafford, S. (2000) Multivariate statistics for wildlife and ecology research. 283 pp. New York, Springer.
Mattiucci, S. & Nascetti, G. (2006) Molecular systematics, phylogeny and ecology of anisakid nematodes of the genus Anisakis Dujardin, 1845: an update. Parasité 13, 99–113.
Mattiucci, S., Nascetti, G., Bullini, L., Orecchia, P. & Paggi, L. (1986) Genetic structure of Anisakis physeteris and its differentiation from the Anisakis simplex complex (Ascaridida: Anisakidae). Parasitology 93, 383–387.
Mattiucci, S., Nascetti, G., Cianchi, R., Paggi, L., Arduino, P., Margolis, L., Brattey, J., Webb, S., D'Amelio, S., Orecchia, P. & Bullini, L. (1997) Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae). Journal of Parasitology 83, 401416.
Mattiucci, S., Paggi, L., Nascetti, G., Ishikura, H., Kikuchi, K., Sato, N., Cianchi, R. & Bullini, L. (1998) Allozyme and morphological identification of Anisakis, Contracaecum, and Pseudoterranova from Japanese waters (Nematoda, Ascaridoidea). Systematic Parasitology 40, 81–92.
Mattiucci, S., Paggi, L., Nascetti, G., Abollo, E., Webb, S.C., Pascual, S., Cianchi, R. & Bullini, L. (2001) Genetic divergence and reproductive isolation between Anisakis brevispiculata and Anisakis physeteris (Nematoda: Anisakidae). International Journal for Parasitology 31, 9–14.
Mattiucci, S., Paggi, L., Nascetti, G., Portes Santos, C., Costa, G., Di Beneditto, A.P., Ramos, R., Argyrou, M., Cianchi, R. & Bullini, L. (2002) Genetic markers in the study of Anisakis typica (Diesing, 1860): larval identification and genetic relationships with other species of Anisakis Dujardin, 1845 (Nematoda: Anisakidae). Systematic Parasitology 51, 159–170.
Mattiucci, S., Abaunza, P., Ramadori, L. & Nascetti, G. (2004) Genetic identification of Anisakis larvae in European hake from Atlantic and Mediterranean waters for stock recognition. Journal of Fish Biology 65, 495510.
Mattiucci, S., Nascetti, G., Dailey, M., Webb, S.C., Cianchi, R. & Bullini, L. (2005) Evidence for a new species of Anisakis Dujardin, 1845: morphological description and genetic relationships between congeners (Nematoda: Anisakidae). Systematic Parasitology 61, 157171.
Mattiucci, S., Farina, V., Campbell, N., MacKenzie, K., Ramos, P., Pinto, A.L., Abaunza, P. & Nascetti, G. (2007) Anisakis spp. larvae (Nematoda: Anisakidae) from Atlantic horse mackerel: their genetic identification and use as biological tags for host stock characterization. Fisheries Research, in press.
Nadler, S., D'Amelio, S., Dailey, M.D., Paggi, L., Siu, S. & Sakanari, J.A. (2005) Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from Northern Pacific marine mammals. Journal of Parasitology 95 (6), 14231429.
Nascetti, G., Paggi, L., Orecchia, P., Smith, J.W., Mattiucci, S. & Bullini, L. (1986) Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from the Mediterranean and North East Atlantic. International Journal for Parasitology 16, 633640.
Paggi, L., Nascetti, G., Webb, S.C., Mattiucci, S., Cianchi, R. & Bullini, L. (1998) A new species of Anisakis Dujardin, 1845 (Nematoda, Anisakidae) from beaked whales (Ziphiidae): allozyme and morphological evidence. Systematic Parasitology 40, 161174.
Pannacciulli, F.G., Bishop, J.D.D. & Hawkins, S.J. (1997) Genetic structure of population of two species of Chtamalus (Crustacea: Cirripedia) in the north-east Atlantic and Mediterranean. Marine Biology 128, 73–82.
Pontes, T., D'Amelio, S., Costa, G. & Paggi, L. (2005) Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species. Journal of Parasitology 91 (6), 14301434.
Reeb, C.A. & Block, B. (2006) Ten microsatellite loci show that Mediterranean swordfish are genetically unique from other populations worldwide. Collection Volume of the Scientific Papers of ICCAT 2006.
Rios, C., Sanz, S., Saavedra, C. & Pena, J.B. (2002) Allozyme variation of populations of scallops, Pecten jaboeus (L.) and P. maximus (L.) (Bivalvia: Pectenidae), across the Almeria-Oran front. Journal of Experimental Marine Biology and Ecology 267, 223244.
Rósza, L., Reiczigel, J. & Majoros, G. (2000) Quantifying parasites in samples of hosts. Journal of Parasitology 86, 228232.
Valentini, A., Mattiucci, S., Bondanelli, P., Webb, S., Mignucci-Giannone, A., Colom-Llavina, M. & Nascetti, G. (2006) Genetic relationships among Anisakis species. (Nematoda: Anisakidae) inferred from mitochondrial cox-2 sequences, and comparison with allozyme data. Journal of Parasitology 92 (1), 156–166.
Velasco, F., Olaso, I. & Sanchez, F. (2001) The role of cephalopods as forage for the demersal fish community in the southern Bay of Biscay. Fisheries Research 52, 65–77.
Waldman, J.R. (1999) The importance of comparative studies in stock analysis. Fisheries Research 43, 237246.


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