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Diagnostic Mr31/32 000 Schistosoma mansoni proteins (Sm31/32): Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium

Published online by Cambridge University Press:  05 June 2009

Mohamed A. Idris  and
Andreas Ruppel
Mohamed A. Idris
Affiliation:
Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany
Andreas Ruppel*
Affiliation:
Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany
*
†Dr. A. Ruppel, Institut für Tropenhygiene, Im Neuenheimer Feld 324, D–6900 Heidelberg, F.R.G.
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Abstract

Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32 000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n=123) and all but one from those with urinary schistosomiasis (n=35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are close to 100%. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium. Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium-patient sera and as a broad band with many S. mansoni-sera suggesting that at least two components are present in the molecular weight region of Mr31/32 000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.

Keywords

diagnostic proteinsimmunoblotspatient seraSchistosoma haematobiumSchistosoma mansoniserodiagnosisSm31/32
Type
Research Papers
Information
Journal of Helminthology , Volume 62 , Issue 2 , June 1988 , pp. 95 - 101
Copyright
Copyright © Cambridge University Press 1988

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