Hostname: page-component-76fb5796d-wq484 Total loading time: 0 Render date: 2024-04-28T17:05:07.564Z Has data issue: false hasContentIssue false

Description of Acromoldavicus xerophilus n. sp. (Nematoda, Rhabditida, Elaphonematidae) from the southern Iberian Peninsula, including a key to species of the genus

Published online by Cambridge University Press:  02 February 2024

M.-C. Robles
Affiliation:
Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus “Las Lagunillas” s/n. 23071-Jaén, Spain
J. Abolafia*
Affiliation:
Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus “Las Lagunillas” s/n. 23071-Jaén, Spain
*
Corresponding author: Joaquín Abolafia; Email: abolafia@ujaen.es

Abstract

A new species of the genus Acromoldavicus is described from coastal sand dunes and sandy soil in the southeast of the Iberian Peninsula. Acromoldavicus xerophilus n. sp. is characterized by its 557–700 μm body length, cuticle tessellated, lip region with three pairs of expanded lips bearing a large labial expansion, primary axils bearing guard processes with two different morphology, secondary axils lacking guard processes, stoma short and tubular with prostegostom bearing prominent rhabdia directed towards the stoma lumen, female reproductive system monodelphic-prodelphic, post-vulval sac 0.6–0.9 times body diameter, rectum very large, female tail short with biacute terminus and males unknown. The description, light micrographs, scanning electron microscope images, illustrations, and molecular analyses are provided. Molecular analyses (based on 18S and 28S rDNA) revealed its relationship with some species of the genera Cephalobus (18S tree), Nothacrobeles, Paracrobeles, and Spinocephalus (28S tree). Keys to species identification of this genus are also included.

Type
Research Paper
Copyright
© The Author(s), 2024. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abolafia, J (2015). A low-cost technique to manufacture a container to process meiofauna for scanning electron microscopy. Microscopy Research and Technique 78, 9, 771776. https://doi.org/10.1002/jemt.22538CrossRefGoogle ScholarPubMed
Abolafia, J (2022). Extracción y procesado de nematodos de muestras de suelos de cuevas y otros hábitats. Monografías Bioespeleológicas 16, 617.Google Scholar
Abolafia, J, Divsalar, N, Panahi, H, Shokoohi, E (2014). Description of Paracrobeles deserticola sp. n. and Nothacrobeles hebetocaudatus sp. n. (Nematoda: Rhabditida: Cephalobidae) from Iran and the phylogenetic relationships of these two species. Zootaxa 3827, 1, 119. https://doi.org/10.11646/zootaxa.3827.1.1CrossRefGoogle Scholar
Abolafia, J, Hosseinvand, M, Eskandari, A (2021). Description of Spinocephalus tessellatus n. gen., n. sp. (Rhabditida, Cephalobidae) from Iran, a nematode with a new morphological pattern at lip region, Journal of Nematology 53, 116. https://doi.org/10.21307/jofnem-2021-078Google Scholar
Abolafia, J, Peña-Santiago, R (2017). On the identity of Chiloplacus magnus Rashid and Heyns, 1990 and C. insularis Orselli and Vinciguerra, 2002 (Rhabditida: Cephalobidae), two confusable species. Nematology 19, 9, 10171034. https://doi.org/10.1163/15685411-0000312CrossRefGoogle Scholar
Abolafia, J, Ruiz-Cuenca, AN (2021). Phoretic invertebrates associated with Rhynchophorus ferrugineus (Coleoptera: Curculionidae) in Canarian date palm from southern Spain. Journal of Natural History 54, 22652284. https://doi.org/10.1080/00222933.2020.1842930CrossRefGoogle Scholar
Andrássy, I (1976). Evolution as a Basis for the Systematization of Nematodes. London: Pitman Publishing.Google Scholar
Archidona-Yuste, A, Navas-Cortés, JA, Cantalapiedra-Navarrete, C, Palomares-Rius, JE, Castillo, P (2016). Unravelling the biodiversity and molecular phylogeny of needle nematodes of the genus Longidorus (Nematoda: Longidoridae) in olive and a description of six new species. PLoS ONE 11, 1, e0147689. https://doi.org/10.1371/journal.pone.0147689CrossRefGoogle Scholar
Baermann, G (1917). Eine einfache Methode zur Auffindung von Ankylostomum (Nematoden) Larven in Erdproben. Geneeskundig Tijdschrift voor Nederlandsch-Indië 57, 131137.Google Scholar
Baldwin, JG, De Ley, IT, Mundo-Ocampo, M, De Ley, P, Nadler, SA, Gebre, M (2001). Acromoldavicus mojavicus n. sp. (Nematoda: Cephaloboidea) from the Mojave Desert, California. Nematology 3, 4, 343353. https://doi.org/10.1163/156854101317020268Google Scholar
Bastian, HC (1865). Monograph on the Anguillulidae, or free nematoids, marine, land, and freshwater; with descriptions of 100 new species. Transactions of the Linnean Society of London, Zoology 25, 73184. https://doi.org/10.1111/j.1096-3642.1865.tb00179.xCrossRefGoogle Scholar
Boström, S (1985). Description of Acrobeloides emarginatus (de Man, 1880) Thorne, 1937 and proposal of Acrolobus n. gen. (Nematoda: Cephalobidae). Revue of Nématologie 8, 335340.Google Scholar
Boström, S (1989). A scanning electron microscope study of juveniles of Acromoldavicus Nesterov (Nematoda: Cephalobidae) from Greece. Nematologica Mediterranea 17, 1, 2729.Google Scholar
Boström, S (1992). Some Cephalobidae (Nematoda: Rhabditida) from Crete, Greece. Fundamental and Applied Nematology 15, 289295.Google Scholar
Castillo, P, Vovlas, N, Subbotin, SA, Troccoli, A (2003). A new root-knot nematode, Meloidogyne baetica n. sp. (Nematoda: Heteroderidae), parasitizing wild olive in Southern Spain. Phytopathology 93, 9, 10931102. https://doi.org/10.1094/PHYTO.2003.93.9.1093CrossRefGoogle Scholar
Darriba, D, Taboada, GL, Doallo, R, Posada, D (2012). jModelTest 2: More models, new heuristics and parallel computing. Nature Methods 9, 8, 772. https://doi.org/10.1038/nmeth.2109CrossRefGoogle ScholarPubMed
De Ley, P, Blaxter, M (2002). Systematic position and phylogeny. In Lee, DL (ed.). The Biology of Nematodes. London: Taylor & Francis, 130.Google Scholar
De Ley, P, Felix, AM, Frisse, LM, Nadler, SA, Sternberg, PW, Thomas, WK (1999). Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1, 591612. https://doi.org/10.1163/156854199508559CrossRefGoogle Scholar
De Ley, P, van de Velde, MC, Mounport, D, Baujard, P, Coomans, A (1995). Ultrastructure of the stoma in Cephalobidae, Panagrolaimidae and Rhabditidae, with a proposal for a revised stoma terminology in Rhabditida (Nematoda). Nematologica 41, 1–4, 153182. https://doi.org/10.1163/003925995X00143CrossRefGoogle Scholar
de Maeseneer, J, d’Herde, J (1963). Méthodes utilisées por l’étude des anguillules libres du sol. Revue Agricultural, Bruxelle 16, 441447.Google Scholar
de Man, JG (1881). Die Einheimischen, frei in der reinen Erde und im süssen Wasser lebende Nematoden monographisch bearbeitet. Vorläufiger Bericht und descriptive-systematischer Theil. Tijdschrift van de Nederlandse Dierkundige Vereeniging 5, 1104.Google Scholar
Filipjev, IN (1934). The classification of the free-living nematodes and their relation to the parasitic nematodes. Smithsonian Miscellaneous Collections 89, 163.Google Scholar
Heyns, J (1962). Elaphonema mirabile n. gen., n. sp. (Rhabditida), a remarkable new nematode from South Africa. Proceedings of the Helminthological Society of Washington 29, 128130.Google Scholar
Holterman, M, van der Wurff, A, van den Elsen, S, van Megen, H, Bongers, T, Holovachov, O, Bakker, J, Helder, J (2006). Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 9, 17921800. https://doi.org/10.1093/molbev/msl044CrossRefGoogle ScholarPubMed
Iliev, I, Ilieva, Z, Mitor, M (2003). Morphometrics of nematodes of the Tisata Reserve: Ordo Rhabditida Chitwood, 1933. Silva Balcanica 3, 3554.Google Scholar
Kumar, S, Stecher, G, Tamura, K (2016). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 7, 18701874. https://doi.org/10.1093/molbev/msw054CrossRefGoogle ScholarPubMed
Karegar, A, De Ley, P, Geraert, E (1997). A detailed morphological study of Acromoldavicus skrjabini (Nesterov and Lisetskaya, 1965) Nesterov, 1970 (Nematoda: Cephaloboidea) from Iran and Spain. Fundamental and Applied Nematology 20, 277283.Google Scholar
Larget, B, Simon, DL (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16, 750759. https://doi.org/10.1093/oxfordjournals.molbev.a026160CrossRefGoogle Scholar
Mehdizadeh, S, Shokoohi, E (2013). The genera Nothacrobeles Allen and Noffsinger, 1971 and Zeldia Thorne, 1937 (Nematoda: Rhabditida: Cephalobidae) from southern Iran, with description of N. abolafiai sp. n. Zootaxa 3637, 325340. https://doi.org/10.11646/zootaxa.3637.3.5CrossRefGoogle Scholar
Nesterov, PI (1970). [Acromoldavicus n. gen. and redescription of the species Acrobeloides skjabini Nesterov and Lisetskaya, 1965 (Nematoda, Cephalobidae). Parazity Zhivotnykh i Rasteniy 5, 134138. [in Russian]Google Scholar
Nesterov, PI (1979). [Plant-parasitic and free-living nematodes in south-western USSR]. Kishinev: Shtiintsa. [in Russian]Google Scholar
Nesterov, PI, Lisetskaya, LF (1965). [The nematode fauna of some soils in the Moldavian SSR]. Parazity Zhivotnykh i Rasteniy 1, 316. [in Russian]Google Scholar
Nunn, GB (1992). Nematode Molecular Evolution. An Investigation of Evolutionary Patterns among Nematodes based upon DNA Sequences. Ph.D. dissertation, University of Nottingham, UK.Google Scholar
Rambaut, A (2018). Figtree, V1.4.4. Available at: https://github.com/rambaut/figtree/releases/tag/v1.4.4 (accessed 28 October 2023).Google Scholar
Ronquist, F, Teslenko, M, van der Mark, P, Ayres, DL, Darling, A, Höhna, S, Larget, B, Liu, L, Suchard, MA, Huelsenbeck, JP (2012). MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 3, 539542. https://doi.org/10.1093/sysbio/sys029CrossRefGoogle ScholarPubMed
Ruiz-Cuenca, AN, Abolafia, J (2020). SEM study of a topotype population of Paracrobeles psammophilus Navarro and Lluch, 1999 (Rhabditida: Cephalobidae) and its taxonomic implications. Nematology 22, 6, 697712. https://doi.org/10.1163/15685411-00003333CrossRefGoogle Scholar
Sanger, F, Nicklen, S, Coulson, AR (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A. 74, 12, 54635467. https://doi.org/10.1073/pnas.74.12.5463CrossRefGoogle ScholarPubMed
Siddiqi, MR (1964). Studies on Discolaimus spp. (Nematoda: Dorylaimidae) from India. Zeitschrift für Zoologische Systematik und Evolutionsforschun 2, 1–3, 174184. https://doi.org/10.1111/j.1439-0469.1964.tb00720.xCrossRefGoogle Scholar
Susulovsky, A, Boström, S, Holovachov, O (2001). Description of Acromoldavicus skrjabini (Nesterov and Lisetskaya, 1965) Nesterov, 1970 from Israel and the Ukraine, and redescription of Kirjanovia discoidea Ivanova, 1969 (Cephalobina: Elaphonematidae). Journal of Nematode Morphology and Systematics 3, 151163.Google Scholar
Thompson, JD, Higgins, DG, Gibson, TJ (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 22, 46734680. https://doi.org/10.1093/nar/22.22.4673CrossRefGoogle ScholarPubMed
Thorne, G (1925). The genus Acrobeles von Linstow, 1877. Transactions of the American Microscopical Society 44, 171210.CrossRefGoogle Scholar
Thorne, G (1937). A revision of the nematode family Cephalobidae Chitwood and Chitwood, 1934. Proceedings of the Helminthological Society of Washington 4, 116.Google Scholar
Timm, RW (1971). Antarctic soil and freshwater nematodes from the McMurdo Sound Region. Proceedings of the Helminthological Society of Washington 38, 4252.Google Scholar