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Demonstration of collagenase activity in adult Strongyloides ratti (Nematoda: Strongyloididae) and its absence in the infective larvae

  • Guta Wertheim (a1), Sarah Lustigman (a1), Hermina Silberman (a1) and Shmuel Shoshan (a2)

Abstract

Larvae and adults of Strongyloides ratti were examined for collagenolytic activity on 14C proline-labelled, native, guinea-pig skin collagen substrate. The activity was measured by determining either the amount of hydroxyproline released or the amount of radioactivity in the solubilized fraction of the collagen substrate. Bacterial collagenase was used for enzyme control and trypsin served as substrate control. No collagenolytic activity was found in living larvae, their extracts or metabolites. The collagenolytic activity of the metabolites of adult worms appeared weak, whereas that of the extracts of the adults was pronounced. It is suggested that collagenase is active in the adult females at the time of migration in the intestinal mucosa during oviposition.

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Fülleborn, F. (1929) On the larval migration of some parasite nematodes in the body of the host and its biological significance. Journal of Helminthology, 7, 1526.
Gross, J. (1958) Studies on the formation of collagen. Journal of Experimental Medicine, 107, 247263.
Israeli, C., Silberman, H. & Wertheim, G. (1982) Observations on substrate penetration by infective larvae of Strongyloides ratti. Israel Journal of Medical Sciences, 18, 313 (abstract).
Lee, D. I. (1972) Penetration of mammalian skin by the infective larva of Nippostrongylus brasiliensis. Parasitology, 65, 499505.
Lewert, R. M. (1958) Invasiveness of helminth larvae. In: Symposium of Resistance and Immunity of Parasitic Infections.Rice Institute Pamphlet, 45, 97113.
Lewert, R. M. & Lee, C. (1956) Quantitative studies of the collagenase-like enzymes of cercariae of Schistosoma mansoni and the larvae of Strongyloides ratti. Journal of Infectious Diseases, 99, 114.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin reagent. Journal of Biological Chemistry, 193, 265275.
Matthews, B. E. (1975) Mechanism of skin penetration by Ancylostoma tubaeforme larvae. Parasitology, 70, 2538.
Matthews, B. E. (1977) The passage of larval helminths through tissue barriers. In: Parasitic Invasion (Taylor, A. E. R. and Muller, R.. Editors) Vol. 15. pp. 93119. Blackwell Scientific Publishers: Oxford.
Milleman, R. E. & Thonard, J. C. (1959) Protease activity in schistosome cercariae. Experimental Parasitology, 8, 129136.
Sandground, J. H. (1925) Speciation and specificity in the nematode genus Strongyloides. Journal of Parasitology, 12, 5980.
Stegeman, H. & Stalder, K. (1967) Determination of hydroxyproline. Clinica Chemica Ada, 18, 267273.
Stirewalt, M. A. (1966) Skin penetration of helminths. In: Biology of Parasites (Editor Soulsby, J. L.) pp.4159. Academic Press: New York and London.
Wertheim, G. (1970) Experimental concurrent infections with Strongyloides ratti and S. venesuelensis in laboratory rats. Parasitology, 61, 389395.

Demonstration of collagenase activity in adult Strongyloides ratti (Nematoda: Strongyloididae) and its absence in the infective larvae

  • Guta Wertheim (a1), Sarah Lustigman (a1), Hermina Silberman (a1) and Shmuel Shoshan (a2)

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