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Characterization of the Opisthorchis viverrini genome

  • R. Sermswan (a1), S. Mongkolsuk (a2) and S. Sirisinha (a1)

Abstract

The methylations of trematode genomic DNA were analyzed using restriction enzymes and Southern blot hybridization. Restriction enzymes MspI, HpaII, HhaI were used to probe CpG methylation while MboI, Sau3A, DpnI were used for A methylation. The results revealed that Opisthorchis viverrini, Fasciola gigantica and Gigantocotyle siamensis had neither CpG nor A methylations. The presence of highly repeated DNA elements was also demonstrated in O. viverrini genomic DNA.

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Keywords

Characterization of the Opisthorchis viverrini genome

  • R. Sermswan (a1), S. Mongkolsuk (a2) and S. Sirisinha (a1)

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