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Characterization of the Opisthorchis viverrini genome

Published online by Cambridge University Press:  05 June 2009

R. Sermswan ,
S. Mongkolsuk  and
S. Sirisinha
R. Sermswan
Affiliation:
Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand
S. Mongkolsuk
Affiliation:
Departments of Microbiology and Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand
S. Sirisinha*
Affiliation:
Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand
*
**To whom correspondence should be addressed.
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Abstract

The methylations of trematode genomic DNA were analyzed using restriction enzymes and Southern blot hybridization. Restriction enzymes MspI, HpaII, HhaI were used to probe CpG methylation while MboI, Sau3A, DpnI were used for A methylation. The results revealed that Opisthorchis viverrini, Fasciola gigantica and Gigantocotyle siamensis had neither CpG nor A methylations. The presence of highly repeated DNA elements was also demonstrated in O. viverrini genomic DNA.

Keywords

Opisthorchis viverrinigenomeDNArestriction enzymes
Type
Research Note
Information
Journal of Helminthology , Volume 65 , Issue 1 , March 1991 , pp. 51 - 54
Copyright
Copyright © Cambridge University Press 1991

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References

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