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Characterization of Oesophagostomum asperum and O. columbianum by internal transcribed spacers of nuclear ribosomal DNA

  • G.H. Zhao (a1), B. Hu (a1), J.K. Song (a1), Y.Q. Jia (a1), H.M. Li (a1), C.R. Wang (a2), Q. Lin (a1), Q.X. Xu (a3), S.K. Yu (a1) and Y. Deng (a4)...

Abstract

In the present study, the internal transcribed spacers (ITS) of ribosomal DNA (rDNA) of Oesophagostomum asperum and O. columbianum were amplified and sequenced. The ITS-1, 5.8S and ITS-2 rDNA sequences of O. asperum were 374 bp, 153 bp and 259 bp in length, respectively, and the corresponding sequences of O. columbianum were 259, 153 and 218 bp in length, respectively. Sequence differences in the ITS-1 and ITS-2 rDNA between the two Oesophagostomum species were 9.5–10.2% and 12.7–13.9%, respectively. Sequence differences in the ITS-1 and ITS-2 rDNA among members of the genus Oesophagostomum were 2.5–11.6% and 6.8–22.3%, respectively. Based on genetic markers in the ITS rDNA, an effective polymerase chain reaction (PCR) approach was developed to differentiate O. columbianum from O. asperum with a sensitivity of 0.2 ng/μl DNA. Since accurate characterization of parasites at different taxonomic levels is essential for population genetic studies and control of parasitosis, the present findings have important implications for studying epidemiology, taxonomy and population biology, as well as for the control of oesophagostomiasis.

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Corresponding author

*Fax: +86-29-87081762. E-mails: yusanke15@sohu.com (S.K. Yu); dengyan_2003@126.com (Y. Deng)

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Acevedo-Ramírez, P.M., Quiroz-Romero, H., Cruz-Mendoza, I., Ulloa-Arvizu, R. & Ibarra-Velarde, F. (2012) Gastrointestinal nematodes in rotationally grazing ewes in the mountainous region of central Mexico. Journal of Helminthology 2, 17.
Atopkin, D.M. (2011) Genetic characterization of the Psilotrema (Digenea: Psilostomatidae) genus by partial 28S ribosomal DNA sequences. Parasitology International 60, 541543.
Biffa, D., Jobre, Y. & Chaka, H. (2006) Ovine helminthosis, a major health constraint to productivity of sheep in Ethiopia. Animal Health Research Reviews 7, 107118.
Blouin, M.S. (2002) Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. International Journal for Parasitology 32, 527531.
Bott, N.J., Campbell, B.E., Beveridge, I., Chilton, N.B., Rees, D., Hunt, P.W. & Gasser, R.B. (2009) A combined microscopic-molecular method for the diagnosis of strongylid infections in sheep. International Journal for Parasitology 39, 12771287.
Bowman, D.D. (2009) Georgis' parasitology for veterinarians. 9th edn. pp. 176177. Missouri, Saunders.
Bremner, K.C., Keith, R.K. & Winks, R. (1976) Age resistance of cattle to the nodular worm Oesophagostomum radiatum. Research in Veterinary Science 20, 350351.
Burland, T.G. (2000) DNASTAR's Lasergene sequence analysis software. Methods in Molecular Biology 132, 7191.
Cenci, F.B., Louvandini, H., McManus, C.M., Dell'Porto, A., Costa, D.M., Araújo, S.C., Minho, A.P. & Abdalla, A.L. (2007) Effects of condensed tannin from Acacia mearnsii on sheep infected naturally with gastrointestinal helminthes. Veterinary Parasitology 144, 132137.
Chilton, N.B., Gasser, R.B. & Beveridge, I. (1997) Phylogenetic relationships of Australian strongyloid nematodes inferred from ribosomal DNA sequence data. International Journal for Parasitology 27, 14811494.
Dai, R.S., Liu, G.H., Song, H.Q., Lin, R.Q., Yuan, Z.G., Li, M.W., Huang, S.Y., Liu, W. & Zhu, X.Q. (2012) Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China. Journal of Helminthology 86, 245251.
Dobson, R.J., Barnes, E.H., Birclijin, S.D. & Gill, J.H. (2002) The survival of Ostertagia circumcincta and Trichostrongylus colubriformis in faecal culture as a source of bias in apportioning egg counts to worm species. International Journal for Parasitology 22, 10051008.
Eguale, T., Chaka, H. & Gizaw, D. (2009) Efficacy of albendazole against nematode parasites isolated from a goat farm in Ethiopia: relationship between dose and efficacy in goats. Tropical Animal Health and Production 41, 12671273.
Gasser, R.B. & Newton, S.E. (2000) Genomic and genetic research on bursate nematodes: significance, implications and prospects. International Journal for Parasitology 30, 509534.
Gasser, R.B., Woods, W.G., Huffman, M.A., Blotkamp, J. & Polderman, A.M. (1999) Molecular separation of Oesophagostomum stephanostomum and Oesophagostomum bifurcum (Nematoda: Strongyloidea) from non-human primates. International Journal for Parasitology 29, 10871091.
Jefferies, R., Shaw, S.E., Willesen, J., Viney, M.E. & Morgan, E.R. (2010) Elucidating the spread of the emerging canid nematode Angiostrongylus vasorum between Palaearctic and Nearctic ecozones. Infection, Genetics and Evolution 10, 561568.
Lai, M., Zhou, R.Q., Huang, H.C. & Hu, S.J. (2011) Prevalence and risk factors associated with intestinal parasites in pigs in Chongqing, China. Research in Veterinary Science 91, e121e124.
Lin, R.Q., Zhu, X.Q., Wei, D.X., Deng, Y., Liu, W., Song, H.Q., Li, A.X. & Lun, Z.R. (2007) Characterization of Oesophagostomum spp. from pigs in China by PCR-based approaches using genetic markers in the internal transcribed spacers of ribosomal DNA. Parasitology Research 101, 351356.
Lin, R.Q., Ai, L., Zou, F.C., Verweij, J.J., Jiang, Q., Li, M.W., Song, H.Q. & Zhu, X.Q. (2008) A multiplex PCR tool for the specific identification of Oesophagostomum spp. from pigs. Parasitology Research 103, 993997.
McManus, D.P. & Bowles, J. (1996) Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. International Journal for Parasitology 26, 687704.
Newton, L.A., Chilton, N.B., Monti, J.R., Bjørn, H., Várady, M., Christensen, C.M. & Gasser, R.B. (1997) Rapid PCR-based delineation of the porcine nodular worms, Oesophagostomum dentatum and O. quadrispinulatum. Molecular Cell Probes 11, 149153.
Newton, L.A., Chilton, N.B., Beveridge, I. & Gasser, R.B. (1998) Systematic relationships of some members of the genera Oesophagostomum and Chabertia (Nematoda: Chabertiidae) based on ribosomal DNA sequence data. International Journal for Parasitology 28, 17811789.
Orosová, M., Ivica, K.H., Eva, B. & Marta, S. (2010) Chromosomal characteristics of multiple rDNA clusters and intragenomic variability of ribosomal ITS2 in Caryophyllaeides fennica (Cestoda). Parasitology International 59, 351357.
Page, R.D. (1996) TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357358.
Roeber, F., Jex, A.R., Campbell, A.D., Campbell, B.E., Anderson, G.A. & Gasser, R.B. (2011) Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections. Infection, Genetics and Evolution 11, 849854.
Romstad, A., Gasser, R.B., Monti, J.R., Polderman, A.M., Nansen, P., Pit, D.S. & Chilton, N.B. (1997) Differentiation of Oesophagostomum bifurcum from Necator americanus by PCR using genetic markers in spacer ribosomal DNA. Molecular Cell Probes 11, 169176.
Schindler, A.R., de Gruijter, J.M., Polderman, A.M. & Gasser, R.B. (2005) Definition of genetic markers in nuclear ribosomal DNA for a neglected parasite of primates, Ternidens deminutus (Nematoda: Strongylida) – diagnostic and epidemiological implications. Parasitology 131, 539546.
Stewart, T.B. & Gasbarre, L.C. (1989) The veterinary importance of nodular worms (Oesophagostomum spp). Parasitology Today 5, 209213.
Sweeny, J.P., Robertson, I.D., Ryan, U.M., Jacobson, C. & Woodgate, R.G. (2011) Comparison of molecular and McMaster microscopy techniques to confirm the presence of naturally acquired strongylid nematode infections in sheep. Molecular Biochemical Parasitology 180, 6267.
Sweeny, J.P., Robertson, I.D., Ryan, U.M., Jacobson, C. & Woodgate, R.G. (2012) Impacts of naturally acquired protozoa and strongylid nematode infections on growth and faecal attributes in lambs. Veterinary Parasitology 184, 298308.
Swofford, D.L. (2002) PAUP*: phylogenetic analysis using parsimony (and other methods). Sunderland, Massachusetts, Sinauer Associates.
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 15961599.
Tariq, K.A., Chishti, M.Z., Ahmad, F. & Shawl, A.S. (2008) Epidemiology of gastrointestinal nematodes of sheep managed under traditional husbandry system in Kashmir valley. Veterinary Parasitology 158, 138143.
Tariq, K.A., Chishti, M.Z. & Ahmad, F. (2010) Gastro-intestinal nematode infections in goats relative to season, host sex and age from the Kashmir valley, India. Journal of Helminthology 84, 9397.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 48764882.
Wang, C.R., Qiu, J.H., Zhu, X.Q., Han, X.H., Ni, H.B., Zhao, J.P., Zhou, Q.M., Zhang, H.W. & Lun, Z.R. (2006) Survey of helminths in adult sheep in Heilongjiang Province, People's Republic of China. Veterinary Parasitology 140, 378382.
Wang, M. (2002) Veterinary parasitology. 3rd edn. pp. 167169. Beijing, China Agriculture Press.
Yamada, S., Yoshida, A., Yoshida, K., Kuraishi, T., Hattori, S., Kai, C., Naqai, Y., Sakoda, T., Tatara, M., Abe, S. & Fukumoto, S. (2012) Phylogenetic relationships of three species within the family Heligmonellidae (Nematoda; Heligmosomoidea) from Japanese rodents and a lagomorph based on the sequences of ribosomal DNA internal transcribed spacers, ITS-1 and ITS-2. Japanese Journal of Veterinary Research 60, 1521.
Zhu, X.Q., Gasser, R.B., Podolska, M. & Chilton, N.B. (1998) Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International Journal for Parasitology 28, 19111921.

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Characterization of Oesophagostomum asperum and O. columbianum by internal transcribed spacers of nuclear ribosomal DNA

  • G.H. Zhao (a1), B. Hu (a1), J.K. Song (a1), Y.Q. Jia (a1), H.M. Li (a1), C.R. Wang (a2), Q. Lin (a1), Q.X. Xu (a3), S.K. Yu (a1) and Y. Deng (a4)...

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