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Characterization of Oesophagostomum asperum and O. columbianum by internal transcribed spacers of nuclear ribosomal DNA

Published online by Cambridge University Press:  30 November 2012

G.H. Zhao ,
B. Hu ,
J.K. Song ,
Y.Q. Jia ,
H.M. Li ,
C.R. Wang ,
Q. Lin ,
Q.X. Xu ,
S.K. Yu  and
Y. Deng
G.H. Zhao
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province712100, PR China
B. Hu
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province712100, PR China
J.K. Song
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province712100, PR China
Y.Q. Jia
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province712100, PR China
H.M. Li
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province712100, PR China
C.R. Wang
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
Q. Lin
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province712100, PR China
Q.X. Xu
Affiliation:
Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, Guangdong Province510470, PR China
S.K. Yu*
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province712100, PR China
Y. Deng*
Affiliation:
Guangzhou Airport Entry-Exit Inspection and Quarantine Bureau, Guangzhou, Guangdong Province510470, PR China
*
*Fax: +86-29-87081762. E-mails: yusanke15@sohu.com (S.K. Yu); dengyan_2003@126.com (Y. Deng)
*Fax: +86-29-87081762. E-mails: yusanke15@sohu.com (S.K. Yu); dengyan_2003@126.com (Y. Deng)
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Abstract

In the present study, the internal transcribed spacers (ITS) of ribosomal DNA (rDNA) of Oesophagostomum asperum and O. columbianum were amplified and sequenced. The ITS-1, 5.8S and ITS-2 rDNA sequences of O. asperum were 374 bp, 153 bp and 259 bp in length, respectively, and the corresponding sequences of O. columbianum were 259, 153 and 218 bp in length, respectively. Sequence differences in the ITS-1 and ITS-2 rDNA between the two Oesophagostomum species were 9.5–10.2% and 12.7–13.9%, respectively. Sequence differences in the ITS-1 and ITS-2 rDNA among members of the genus Oesophagostomum were 2.5–11.6% and 6.8–22.3%, respectively. Based on genetic markers in the ITS rDNA, an effective polymerase chain reaction (PCR) approach was developed to differentiate O. columbianum from O. asperum with a sensitivity of 0.2 ng/μl DNA. Since accurate characterization of parasites at different taxonomic levels is essential for population genetic studies and control of parasitosis, the present findings have important implications for studying epidemiology, taxonomy and population biology, as well as for the control of oesophagostomiasis.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 1 , March 2014 , pp. 74 - 81
Copyright
Copyright © Cambridge University Press 2012 

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