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Cercarial tail loss in Echinostoma caproni: the influence of in vivo encystment and copper sulphate

Published online by Cambridge University Press:  12 April 2024

B. Fried  and
J.L. Schneck
B. Fried*
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
J.L. Schneck
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
*
*Fax: 610 330 5705 Email: friedb@lafayette.edu
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Abstract

Echinostoma caproni tail loss was studied in vitro in the presence of the toxicant copper sulphate (CuSO4) in concentrations ranging from 1 to 10 000 mg l?1 in standardized artificial spring water (pH 7.4, osmolarity 34 mOsm kg?1 H2O, Ca2+ 20 mg l?1) at 23°C. Tail loss was also studied in the absence of toxicants during in vivo encystment of the cercariae in juvenile Biomphalaria glabrata. As the concentration of CuSO4 increased, the percentage of cercarial tail loss increased. By 2 h in 10 000 mg l?1, 1000 mg l?1 and 100 mg l?1 CuSO4, 50%, 23% and 13%, respectively, of the cercariae had lost their tails. In the in vivo studies, by 1 h PI, 59±5% of cercariae had lost their tails and only 4±1% of the cercariae were actively swimming in the multi-well dishes. At 3 h PI, 72±3% of the cercariae began to form cysts within the snails.

Type
Review Article
Information
Journal of Helminthology , Volume 79 , Issue 2 , June 2005 , pp. 177 - 178
Copyright
Copyright © Cambridge University Press 2005

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References

Fried, B. LaTerra, R. (2002) In vitro and in vivo encystment of the cercariae of Echinostoma caproni . Journal of Parasitology 88: 1124–1129.CrossRefGoogle ScholarPubMed
Hara, I. Hara, S. Fusco, A.C. Salafsky, B. & Shibuya, T. (1993a) Role of calcium ion in Schistosoma mansoni cercarial tail loss induced by unsaturated fatty acids. Journal of Parasitology 79: 504–509.CrossRefGoogle ScholarPubMed
Hara, I. Hara, S. Salafsky, B. & Shibuya, T. (1993b) Schistosoma mansoni: fatty acid-induced cercarial tail loss does not involve eicosanoid production and chemoreceptors. Experimental Parasitology 77: 484–488.CrossRefGoogle Scholar
Idris, N. & Fried, B. (1996) Development, hatching and infectivity of Echinostoma caproni (Trematoda) eggs and histochemical observations on the miracidia. Parasitology Research 82: 136–142.CrossRefGoogle ScholarPubMed
Morley, N.J. Crane, M. & Lewis, J.W. (2002) Toxicity of cadmium and zinc to cercarial tail loss in Diplostomum spathaceum (Trematoda: Diplostomatidae). Parasitology 125: 293–301.CrossRefGoogle Scholar
Morley, N.J. Irwin, S.W.B. & Lewis, J.W. (2003) Pollution toxicity to the transmission of larval digeans through their molluscan hosts. Parasitology 126 S5–S26.CrossRefGoogle Scholar
Reddy, A. Ponder, E.L. & Fried, B. (2004) Effects of copper sulfate on cercariae and metacercariae of Echinostoma caproni and Echinostoma trivolvis and on the survival of Biomphalaria glabrata snails. Journal of Parasitology 90: 1332–1337.CrossRefGoogle ScholarPubMed
Schneck, J.L. & Fried, B. (2004) Effects of snail size on encystment of Echinostoma caproni in juvenile Biomphalaria glabrata (NMRI strain) and observations on the survival of infected snails. Journal of Helminthology 78: 277–279.CrossRefGoogle ScholarPubMed
Ulmer, M.J. (1970) Notes on rearing of snails in the laboratory. pp. 143–144 MacInnis, A.J., Voge, N. (Eds) Experiments and techniques in parasitology. San Francisco, California, W.H. Freeman and Co.Google Scholar
Wiest, P.M. Kossman, R.J. & Tartakoff, A.M. (1989) Determinants of surface membrane maturation during the cercarial-schistosomula transformation of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 41: 70–77.CrossRefGoogle ScholarPubMed