Hostname: page-component-848d4c4894-xfwgj Total loading time: 0 Render date: 2024-06-23T06:20:40.192Z Has data issue: false hasContentIssue false
  • English
  • Français

Biological variation between two Brazilian geographical isolates of Echinostoma paraensei

Published online by Cambridge University Press:  12 April 2024

A. Maldonado Júnior ,
B.K. Zeitone ,
L.A. Amado ,
I.F. Amado ,
J.R. Machado-Silva  and
R.M. Lanfredi
A. Maldonado Júnior*
Affiliation:
Laboratório de Biologia e Controle da Esquistossomose, Departamento de Medicina Tropical, Instituto Oswaldo Cruz, Av. Brasil 4365 Manguinhos, 21 045-900, Rio de Janeiro, Brazil
B.K. Zeitone
Affiliation:
Laboratório de Biologia e Controle da Esquistossomose, Departamento de Medicina Tropical, Instituto Oswaldo Cruz, Av. Brasil 4365 Manguinhos, 21 045-900, Rio de Janeiro, Brazil
L.A. Amado
Affiliation:
Laboratório de Biologia e Controle da Esquistossomose, Departamento de Medicina Tropical, Instituto Oswaldo Cruz, Av. Brasil 4365 Manguinhos, 21 045-900, Rio de Janeiro, Brazil
I.F. Amado
Affiliation:
Laboratório de Helmintologia, Instituto de Biofísica Carlos Filho, CCS, bl G. UFRJ, Ilha do Fundão, 21 949-900, Rio de Janeiro, Brazil
J.R. Machado-Silva
Affiliation:
Laboratório de Helmintologia Romero Lascasas Porto, Departamento de Patologia e Laboratórios, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rua Prof Manuel de Abreu, 444, 50 andar, Rio de Janeiro, Brazil
R.M. Lanfredi
Affiliation:
Laboratório de Helmintologia, Instituto de Biofísica Carlos Filho, CCS, bl G. UFRJ, Ilha do Fundão, 21 949-900, Rio de Janeiro, Brazil
*
*Fax: (011) 55 21 22803740 E-mail: maldonad@ioc.fiocruz.br
Get access Rights & Permissions [Opens in a new window]

Abstract

The biological behaviour and morphometric data from two allopatric isolates of Echinostoma paraensei (Rio Bonito – RB and Sumidouro – SU) collected from naturally infected Nectomys squamipes from two secluded Atlantic Forest fragments were studied. Mice that had been experimentally infected with ten encysted metacercariae of each isolate were monitored weekly in two trials to analyse worm burden and the kinetics of worm distribution along the intestine. The total number of uterine eggs, wet weights and measurements of the worms and body, acetabulum, testes and ovaries were also analysed. The RB isolate showed a higher worm burden, 7.7±0.8, and a longer life span, 16 weeks, compared to a worm burden of 5.8±1.1 and life span of 9 weeks for the SU isolate. Worms of the RB isolate were clustered in the duodenum and in the bile duct while the SU isolate worms were dispersed along the small intestine of infected mice. Both isolates developed similarly as regards morphometric data and wet weight, although the total number of uterine eggs was greater in RB. The degree of intraspecific variation observed in the worm distribution along the intestine, worm burden and life span raises questions regarding the use of these criteria for species differentiation. These findings suggest that variation in biological parameters found between the E. paraensei isolates could result from geographical isolation and, in particular, the environmental conditions of transmission. Further studies on E. paraensei polulations from different forest fragments will contribute towards an understanding of the speciation of this parasite.

Type
Review Article
Information
Journal of Helminthology , Volume 79 , Issue 4 , December 2005 , pp. 345 - 351
Copyright
Copyright © Cambridge University Press 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brunet, L.R., Joseph, S., Dunne, D.W. & Fried, B. (2000) Immune response during the acute stages of infection with the intestinal trematode Echinostoma caproni . Parasitology 120, 565–571.CrossRefGoogle ScholarPubMed
Christensen, N.Ø, Odaibo, A.B. Simonsen, P.E. (1988) Echinostoma population regulation in experimental rodent definitive hosts. Parasitology Research 75, 83–87.CrossRefGoogle ScholarPubMed
Doy, T.G. & Hughes, D.L. (1984) Early migration of innate Fasciola hepatica and associated liver pathology in cattle. Research in Veterinary Science 37, 219–222.CrossRefGoogle Scholar
Franco, J., Huffman, J.E. & Fried, B. (1988) The effects of crowding on adults of Echinostoma revolutum (Digenea: Echinostomatidae) in experimentally infected golden hamster Mesocricetus auratus. Journal of Parasitology 74, 240–243.CrossRefGoogle Scholar
Fried, B. & Graczyk, T.K. (2000) Echinostomes as experimental models for biological research. 273 pp. Dordrecht, The Netherlands, Kluwer.CrossRefGoogle Scholar
Fried, B. & Huffman, J. (1996) The biology of the intestinal trematode Echinostoma caproni . Advances in Parasitology 38, 311–367.CrossRefGoogle ScholarPubMed
Fried, B., Huffuman, J.E. & Weiss, F. (1990) Single and multiple worm infections of Echinostoma caproni (Trematoda) in the golden hamster. Journal of Helminthology 64, 75–78.CrossRefGoogle ScholarPubMed
Fujino, T. & Fried, B. (1993) Expulsion of Echinostoma trivolvis (Cort, 1914) Kanev, 1985 and retention of E. caproni Richard, 1964, (Trematoda: Echinostomatidae) in C3H mice: pathological, ultrastructural, and cytochemical effects on the host intestine. Parasitology Research 79, 286–292.CrossRefGoogle ScholarPubMed
Howard, R.J., Christie, P.R., Wakelin, D., Wilson, M.M. & Behnke, J.M. (1978) The effect of concurrent infection with Trichinella spiralis and Hymenolepis microstoma in mice. Parasitology 77, 273–279.CrossRefGoogle ScholarPubMed
Hufffman, J.E., Iglesias, D. & Fried, B. (1988) Echinostoma revolutum: pathology of extraintestinal infection in the golden hamster. International Journal for Parasitology 18, 873–874.CrossRefGoogle Scholar
Irwin, S.W.B. (1997) Excystation and cultivation of trematodes. pp. 557–586 in Fried, B. & Graczyk, T.K. (Eds) Advances in trematode biology. Boca Raton, Florida, CRC Press.Google Scholar
Kanev, I. (1994) Life-cycle, delimitation and redescription of Echinostoma revolutum (Frolich, 1802) (Trematoda: Echinostomatidae). Systematic Parasitology 28, 125–144.CrossRefGoogle Scholar
Kanev, I., Dimitrov, V., Radev, V. & Fried, B. (1995) Redescription of Echinostoma trivolvis (Cort, 1924) with a discussion of its identity. Systematic Parasitology 32, 61–70.CrossRefGoogle Scholar
Kaufman, A.R. & Fried, B. (1996) Infectivity, growth, distribution and fecundity of six versus twenty-five metacercarial cyst inoculum of Echinostoma caproni in ICR mice. Journal of Helminthology 68, 203–206.CrossRefGoogle Scholar
Kostadinova, A. & Gibson, D.I. (2001) The systematics of the echinostomes pp.31–38 in Fried, B. & Graczyk, T.K. (Eds) Echinostomes as experimental models for biological research. Dordrecht, Kluwer Academic Publishers.Google Scholar
Lie, K.J. & Basch, P.F. (1967) The life history of Echinostoma paraensei sp.n. (Trematoda: Echinostomatidae). Journal of Parasitology 53, 1192–1199.CrossRefGoogle ScholarPubMed
Lutz, A. (1924) Estudos sobre a evolucão dos endotrematodeos brasileiros. Memorias do Instituto Oswaldo Cruz 17, 55–57.CrossRefGoogle Scholar
Maldonado, A. Jr., Loker, E.S., Morgan, J.A.T., Rey, L. & Lanfredi, R.M. (2001a) Description of the adult worms of a new Brazilian isolate of Echinostoma paraensei (Platyhelminthes: Digenea) from its natural vertebrate host Nectomys squamipes by light and scanning electron microscopy and molecular analysis. Parasitology Research 87, 840–848.Google ScholarPubMed
Maldonado, A. Jr., Vieira, G.O., Garcia, J.S., Rey, L. & Lanfredi, R.M. (2001b) Biological aspects of a new isolate of Echinostoma paraensei (Trematoda: Echinostomatidae): susceptibility of sympatric snails and the natural vertebrate host. Parasitology Research 87, 853–859.Google ScholarPubMed
Maldonado, A. Jr., Vieira, G.O. & Lanfredi, R.M. (2003) Echinostoma luisreyi n. sp. (Platyhelminthes: Digenea) by light and scanning electron microscopy. Journal of Parasitology 89, 800–808.CrossRefGoogle Scholar
McCarthy, A.M. (1990) Speciation of echinostomes: evidence for the existence of two sympatric sibling species in the complex Echinoparyphium recurvatum (Von Linstow 1873) (Digenea: Echinostomatidae). Parasitology 101, 35–42.CrossRefGoogle Scholar
Meece, J.K. & Nollen, P.M. (1996) A comparison of the adult and miracidial stages of Echinostoma paraensei and E. caproni . International Journal for Parasitology 26, 37–43.CrossRefGoogle ScholarPubMed
Morgan, J.A.T. & Blair, D. (1998) Relative merits of nuclear ribossomal internal transcribed spacer and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species (Trematoda). Parasitology 116, 289–297.CrossRefGoogle Scholar
Nollen, P.M. (1990) Echinostoma caproni mating behavior and the timing of development and movement of reproductive cells. Journal of Parasitology 76, 784–789.CrossRefGoogle ScholarPubMed
Pires, A.S., Lira, P.K., Fernandez, A.S., Schittini, G.M. & Oliveira, L.C. (2002) Frequency of movements of small mammals among Atlantic Coastal Forest fragments in Brazil. Biological Conservation 108, 229–237.CrossRefGoogle Scholar
Reddy, A. & Fried, B. (1996) Egg laying in vitro of Echinostoma caproni (Trematoda) in nutritive and non-nutritive media. Parasitology Research 82, 475–478.CrossRefGoogle ScholarPubMed
Sorensen, R.E., Curtis, J. & Minchela, D.J. (1998) Interspecific variation in the rDNA ITS loci 37-collar-spined echinostomes from North America: implications for sequence-based diagnoses and phylogenetics. Journal of Parasitology 84, 992–997.CrossRefGoogle Scholar
Sukhdeo, M.V.K. (1990) Habitat selection by helminths: a hypothesis. Parasitology Today 6, 234–237.CrossRefGoogle ScholarPubMed
Sukhdeo, M.V.K. & Sukhdeo, S.C. (2002) Fixed behaviors and migration in parasitic flatworms. International Journal for Parasitology 32, 329–342.CrossRefGoogle ScholarPubMed
Toledo, R., Espert, A., Carpena, I., Munoz-Antole, C. & Esteban, J.G. (2003) An experimental study of the reproductive success of Echinostoma friedi n. sp. (Trematoda: Echinostomatidae) in the golden hamster. Parasitology 126, 433–441.CrossRefGoogle Scholar
Toledo, R., Munoz-Antole, C. & Esteban, J.G. (2000) Life-cycle of Echinostoma friedi n. sp. (Trematoda: Echinostomatidae) in Spain and a discussion on the relationship within the ‘revolutum’ group based on cercarial chaetotaxy. Systematic Parasitology 45, 199–217.Google ScholarPubMed
Travassos, L., Freitas, J.F. & Kohn, A. (1969) Trematódeos do Brasil. Memórias do Instituto Oswaldo Cruz 67, 1–420.Google Scholar
Trouvé, S. & Coustau, C. (1998) Differences in adult excretory–secretory products between geographical isolates of Echinostoma caproni . Journal of Parasitology 84, 1062–1065.CrossRefGoogle ScholarPubMed
Yamaguti, S. (1971) Synopsis of digenetic trematodes of vertebrates. Vol. 1. Tokyo, Keigaku.Google Scholar