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Behavioural changes and muscle strength in Rattus norvegicus experimentally infected with Toxocara cati and T. canis

Published online by Cambridge University Press:  11 April 2014

S.V. Santos*
Affiliation:
Instituto de Medicina Tropical de São Paulo - LIM 06, Avenida Doutor Eneias de Carvalho Aguiar, 450 - Cerqueira Cesar, São Paulo, SP05403-000, Brazil Faculdade de Ciências Médicas da Santa Casa de São Paulo, Rua Doutor Cesário Mota Jr., 61, Santa Cecília, São Paulo, SP01221-020, Brazil
J.V.L. Moura
Affiliation:
Faculdade de Ciências Médicas da Santa Casa de São Paulo, Rua Doutor Cesário Mota Jr., 61, Santa Cecília, São Paulo, SP01221-020, Brazil
S.A.Z. Lescano
Affiliation:
Instituto de Medicina Tropical de São Paulo - LIM 06, Avenida Doutor Eneias de Carvalho Aguiar, 450 - Cerqueira Cesar, São Paulo, SP05403-000, Brazil
J.M. Castro
Affiliation:
Prefeitura do Município de São Paulo/SUVIS/Vila Maria/Vila Guilherme, Avenida Guilherme, 82, Vila Guilherme, São Paulo, SP02053-000, Brazil
M.C.S.A. Ribeiro
Affiliation:
Faculdade de Ciências Médicas da Santa Casa de São Paulo, Rua Doutor Cesário Mota Jr., 61, Santa Cecília, São Paulo, SP01221-020, Brazil
P.P. Chieffi
Affiliation:
Instituto de Medicina Tropical de São Paulo - LIM 06, Avenida Doutor Eneias de Carvalho Aguiar, 450 - Cerqueira Cesar, São Paulo, SP05403-000, Brazil Faculdade de Ciências Médicas da Santa Casa de São Paulo, Rua Doutor Cesário Mota Jr., 61, Santa Cecília, São Paulo, SP01221-020, Brazil
*

Abstract

Toxocara canis and Toxocara cati are nematode parasites in dogs and cats, respectively, transmitted by ingestion of embryonated eggs, transmammary and transplacental (T. canis) routes and paratenic host predation. Many parasites use mechanisms that change the behaviour of their hosts to ensure continued transmission. Several researchers have demonstrated behavioural changes in mouse models as paratenic hosts for T. canis. However, there have been no studies on behavioural changes in laboratory rats (Rattus norvegicus) experimentally infected with T. cati. This study investigated behavioural changes and muscle strength in male and female rats experimentally infected with T. cati or T. canis in acute and chronic phases of infection. Regardless of sex, rats infected with T. cati showed a greater decrease in muscle strength 42 days post infection compared to rats infected with T. canis. However, behavioural changes were only observed in female rats infected with T. canis.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2014 

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References

Burright, R.G., Donovick, P.J., Dolinsky, Z., Hurd, Y. & Cypress, R. (1982) Behavioral changes in mice infected with Toxocara canis. Journal of Toxicology and Environmental Health 10, 621626.CrossRefGoogle ScholarPubMed
Carola, V., D'Olimpio, F., Brunamonti, E., Mangia, F. & Renzi, P. (2002) Evaluation of the elevated plus-maze and open-field tests for the assessment of anxiety-related behavior in inbred mice. Behavioural Brain Research 134, 4957.CrossRefGoogle ScholarPubMed
Chieffi, P.P., Aquino, R.T.R., Paschoalotti, M.A., Ribeiro, M.C. & Nasello, A.G. (2009a) Muscular strength decrease in Rattus norvegicus experimentally infected by Toxocara canis. Revista do Instituto de Medicina Tropical de Sao Paulo 51, 7375.CrossRefGoogle ScholarPubMed
Chieffi, P.P., Santos, S.V., Queiroz, M.L. & Lescano, S.A.Z. (2009b) Human toxocariasis: contribution by Brazilian researchers. Revista do Instituto de Medicina Tropical de São Paulo 51, 301308.CrossRefGoogle ScholarPubMed
Chieffi, P.P., Aquino, R.T.R., Pasqualotti, M.A., Ribeiro, M.C.S.A. & Nasello, A.G. (2010) Behavioral changes in Rattus norvegicus experimentally infected by Toxocara canis larvae. Revista do Instituto de Medicina Tropical de São Paulo 52, 243246.CrossRefGoogle ScholarPubMed
Coati, N., Schnieder, T. & Epe, C. (2004) Vertical transmission of Toxocara cati Schrank 1788 (Anisakidae) in the cat. Parasitology Research 92, 142146.CrossRefGoogle ScholarPubMed
Cox, D.M. & Holland, C.V. (2001a) The influence of mouse strain, infective dose and larval burden in the brain on activity in Toxocara-infected mice. Journal of Helminthology 75, 2332.CrossRefGoogle ScholarPubMed
Cox, D.M. & Holland, C.V. (2001b) Relationship between three intensity levels of Toxocara canis larvae in the brain effects on exploration, anxiety, learning and memory in the murine host. Journal of Helminthology 75, 3341.CrossRefGoogle ScholarPubMed
De Carli, G.A. (2007) Exames macroscópico e microscópico da amostra fecal fresco e preservada. pp. 2982in De Carli, G.A. (Ed.) Parasitologia clínica – seleção de métodos e técnicas de laboratório para o diagnóstico das parasitoses humanas. São Paulo, Atheneu.Google Scholar
Dolinsky, Z.S., Burright, R.G., Donovick, P.J., Glickman, L.T., Babish, J., Summer, B. & Cypess, R.H. (1981) Behavioural effects of lead and Toxocara canis in mice. Science 213, 11421144.CrossRefGoogle ScholarPubMed
Donovick, P.J. & Burright, R.G. (1987) The consequences of parasitic infection for the behavior of the mammalian host. Environmental Health Perspectives 73, 247250.CrossRefGoogle ScholarPubMed
Eberhard, M.L. & Alfano, E. (1998) Adult Toxocara cati infections in U.S. Children: Report of four cases. American Journal of Tropical Medicine and Hygiene 59, 404406.CrossRefGoogle ScholarPubMed
Fisher, M. (2003) Toxocara cati: an underestimated zoonotic agent. Trends in Parasitology 19, 167170.CrossRefGoogle ScholarPubMed
Guaraldo, L., Chagas, D.A., Konno, A.C., Kom, G.P., Pfiffer, T. & Nasello, A.G. (2000) Hydroalcoholic extract and fractions of Davilla rugosa Poiret: effects on spontaneous motor activity and elevated plus-maze behavior. Journal of Ethnopharmacology 72, 6167.CrossRefGoogle ScholarPubMed
Hall, C.S. (1934) Emotional behavior in the rat. I. Defecation and urination as measures of individual differences in emotionality. Journal of Comparative Psychology 18, 385403.CrossRefGoogle Scholar
Handley, S.L. & Mithani, S. (1984) Effects of alpha-adrenoceptor agonists and antagonists in a maze-exploration model of ‘fear’-motivated behaviour. Naunyn-Schmiedeberg's Archives of Pharmacology 327, 15.CrossRefGoogle Scholar
Hay, J., Arnott, M., Aitken, P.P. & Kendall, A.T. (1986) Experimental toxocariasis and hyperactivity in mice. Zeitschrift für Parasitenkunde 72, 115120.CrossRefGoogle ScholarPubMed
Holland, C.V. & Hamilton, C.M. (2013) The significance of cerebral toxocariasis: a model system for exploring the link between brain involvement, behaviour and the immune response. Journal of Experimental Biology 216, 7883.CrossRefGoogle Scholar
Hrdá, S., Votýpka, J., Kodym, P. & Flegr, J. (2000) Transient nature of Toxoplasma gondii-induced behavioral change in mice. Journal of Parasitology 86, 657663.CrossRefGoogle ScholarPubMed
Lemos, M.T., Amaral, F.A., Dong, K.E., Bittencourt, M.F., Caetano, A.L., Pesquero, J.B., Viel, T.A. & Buck, H.S. (2010) Role of kinin B1 and B2 receptors in memory consolidation during the aging process of mice. Neuropeptides 44, 163168.CrossRefGoogle ScholarPubMed
Lescano, S.A.Z., Queiroz, M.L. & Chieffi, P.P. (2004) Larval recovery of Toxocara canis in organs and tissues of experimentally infected Rattus norvegicus. Memórias do instituto Oswaldo Cruz 99, 627628.CrossRefGoogle ScholarPubMed
Marcondes, F.K., Miguel, K.J. & Melo, L.L. (2001) Estrous cycle influences the response of female rats in the elevated plus-maze test. Physiology & Behavior 74, 435440.CrossRefGoogle ScholarPubMed
Montgomery, K.C. (1955) The relation between fear induced by novel stimulation and exploratory behavior. Journal of Comparative and Physiological Psychology 48, 254260.CrossRefGoogle ScholarPubMed
Mora, S., Dussaubat, N. & Díaz-Vélis, G. (1996) Effect of the estrous cycle and ovarian hormones on behavioral índices of anxiety in female rats. Psychoneuroendocrinology 21, 609620.CrossRefGoogle ScholarPubMed
Olson, L.J. & Rose, J.E. (1966) Effect of Toxocara canis on the ability of white rats to solve maze problems. Experimental Parasitology 19, 7784.CrossRefGoogle ScholarPubMed
Overgaauw, P.A.M. (1997) Aspects of Toxocara epidemiology: Human toxocarosis. Critical Reviews in Microbiology 23, 215231.CrossRefGoogle ScholarPubMed
Pellow, S. & File, S.E. (1986) Anxiolytic and anxiogenic drug effects on exploratory activity in elevated plus-maze: a novel test of anxiety in the rat. Pharmacology Biochemistry and Behavior 24, 525529.CrossRefGoogle ScholarPubMed
Pellow, S., Chopin, P., File, S.E. & Briley, M. (1985) Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. Journal of Neuroscience Methods 14, 149167.CrossRefGoogle Scholar
Poulin, R. (1995) ‘Adaptative’ changes in the behaviour of parasitized animals: a critical review. International Journal for Parasitology 25, 13711383.CrossRefGoogle ScholarPubMed
Poulin, R. (2010) Parasite manipulation of host behavior: an update and frequently asked questions. Advances in the Study of Behavior 41, 151186.CrossRefGoogle Scholar
Queiroz, M.L. & Chieffi, P.P. (2005) Sindrome de larva migrans visceral e Toxocara canis. Arquivos Medicos dos Hospitais da Faculdade de Ciências Medicas da Santa Casa de São Paulo 50, 117120.Google Scholar
Ramos, A., Berton, O., Pierre, M. & Chauloff, F. (1997) A multiple-test study of anxiety-related behaviours in six inbred rat strains. Behavioural Brain Research 85, 5769.CrossRefGoogle ScholarPubMed
Rodgers, R.J. & Johnson, N.J.T. (1995) Factor analysis of spatiotemporal and ethological measures in the murine elevated plus-maze test of anxiety. Pharmacology, Biochemistry and Behavior 52, 297303.CrossRefGoogle ScholarPubMed
Rodgers, R.J., Cao, B.J., Dalvi, A. & Holmes, A. (1997) Animal models of anxiety: an ethological perspective. Brazilian Journal of Medical and Biological Research 30, 289304.CrossRefGoogle ScholarPubMed
Santos, S.V., Lescano, S.Z., Castro, J.M. & Chieffi, P.P. (2009) Larval recovery of Toxocara cati in experimentally infected Rattus norvegicus and analysis of the rat as potential reservoir for this ascarid. Memorias do Instituto Oswaldo Cruz 104, 933934.CrossRefGoogle ScholarPubMed
Sprent, J.F.A. (1956) The life history and development of Toxocara cati (Schrank 1788) in the domestic cat. Parasitology 46, 5478.CrossRefGoogle ScholarPubMed
Walf, A.A. & Frye, C.A. (2007) The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nature Protocols 2, 322328.CrossRefGoogle ScholarPubMed
Webster, J.P. (2007) The effect of Toxoplasma gondii on animal behavior: playing cat and mouse. Schizophrenia Bulletin 33, 752756.CrossRefGoogle ScholarPubMed
Wiseman, R.A. & Lovel, T.W.I. (1969) Human infection with adult Toxocara cati. Archive of the British Medical Journal 3, 454455.Google ScholarPubMed
Xi, W.G. & Jin, L.Z. (1998) A novel method for the recovery of Toxocara canis in mice. Journal of Helminthology 72, 183184.CrossRefGoogle ScholarPubMed